FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179869
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36000.3051186191009341No Hit
CCTCTATGGTGCATCCAACAGGGCCACTGGCATCCCCGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA30740.2605373986434087No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26930.2282456781218932No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25810.21875309886097521No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25120.212904991994874No Hit
GTATCACTGTCAGCAGTTCCATATCTCACCTCAGACGTTCGGCCAAGGGA24210.20519227134537815No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21830.18502054041592753No Hit
GTGTATCACTGTCAGCAGTTCCATATCTCACCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAA21570.182816905944643No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21310.18061327147335848No Hit
CAGTGATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21180.17951145423771622No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATCACTGTCAGCAGTT21180.17951145423771622No Hit
CCATAGAGGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGTTAAGTAGTTGCTGCTAACACTCTGA21170.17942669906574374No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20570.17434138874739483No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAACCTGGTACC19960.16917132325707346No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19230.1629841957030823No Hit
GTGATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18830.15959398882418305No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18690.15840741641656828No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18630.1578988853847334No Hit
GTTCCATATCTCACCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT18550.15722084400895353No Hit
GTCTCTGGTGGCTCCATCAGCGCTAATTATTACTACTACAACTGGGTCCG18310.15518671988161398No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCGGGGATGCCAGTGGCCCTGTT17840.15120322679890733No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG17310.14671120268436583No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16620.1408630958182646No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16500.13984603375459478No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTC15840.134252192404411No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA15830.1341674372324385No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15780.1337436613725761No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC15180.1286583510542272No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14920.1264547165829427No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14870.12603094072308027No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14720.12475961314349306No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATCACTGTCAGCAGTTCCATATCTCA14420.1222169579843186No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13890.11772493386977707No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13850.11738591318188714No Hit
ATATGGAACTGCTGACAGTGATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC13770.11670787180610728No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13710.1161993407742724No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13510.11450423733482278No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13450.11399570630298786No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13350.11314815458326305No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC13110.11111403045592348No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12610.1068762718572994No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCGGGGATGCCA12520.10611347530954708No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12400.1050964132458773No Hit
GTCAGAGTGTTAGCAGCAACTACTTAACCTGGTACCAGCAGAAACCTGGC12240.10374033049431758No Hit
GTGTTAGCAGCAACTACTTAACCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCCTCTATGGTGCAT12220.10357082015037263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.042.6826481
CTAACGC350.001500812138.5754473
GTATCAA25150.035.8580781
TATCAAC28500.031.2730462
ATCAACG29400.030.3092823
TCAACGC29800.029.8973774
CAACGCA29950.029.8615885
AATGGTA6200.029.37212870
AACGCAG31050.028.9123826
GATATAC606.1380776E-428.1470221
TACGTAT1050.007530050427.7496770
CGCCCTT550.00782418827.53495667
ACGCAGA32700.027.4534997
CGCAGAG32700.027.350298
CTAATTC500.00839986127.1374129
TCGAAGG500.008520992527.0578725
GGTCTAG500.008577635527.0211431
AGAGTAC33350.026.91500311
GCAGAGT34000.026.6023339
CTGGAAT11250.025.25219770
Produced by FastQC (version 0.11.5)