FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547934
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75710.48910354059023187No Hit
CTACAGACTCCTCCGGACATGTGCTTTTTTGGCCAGGGGACCAAACTAGA71010.4587404889355748No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62830.4058958586089588No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC53760.34730162913922685No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48400.3126748298054051No Hit
AGTCTGTAGAGCTCGCATGCAGTAATAAATCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT46210.2985269397790862No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG46050.2974933039780766No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45390.293229556298912No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44370.28664012806747574No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG43200.279081666272593No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39650.2561478719376924No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39250.2535637824351684No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37960.2452300937895285No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG36930.2385760633205292No Hit
GTAATGGATACAACTATTTGGATTGGTATGTGCAGAAGCCAGGGCAGTCTCCACAGGTCCTGATCTATTTGGGTT36560.23618578053069444No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36360.23489373577943248No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34600.22352374196832683No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT34530.22307152630538513No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34380.22210249274193858No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGATTTATTACTGCATGCGAGCTCTACAGACT33920.21913078981403597No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGTTTGGTCCCCTGGCCAAAAAAGCACATGTCCGGAGGAGTCTGTAGAG33520.21654670031151202No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT32520.210086476555202No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32340.20892363627906615No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTATGTGCAGAAGCCAGGGCAGTCTC31730.20498289978771703No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31340.20246341252275613No Hit
GTTCAGTGGCAGTGGCTCAGGCACAGATTTTACACTGAAAATCAGCAGAG30210.19516335967812581No Hit
CCTCCGGACATGTGCTTTTTTGGCCAGGGGACCAAACTAGAGATCAAACG30150.1947757462527472No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29470.1903827940984564No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGGGGGCAGATTGTGATGA29320.1894137605350099No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGTTTGGTCC28920.18682967103248588No Hit
GTAATAAATCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGAGCCACTGCCA28580.18463319495534047No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG27990.18082166293911756No Hit
GCTAATGCTCTGGGTCTCTGAATCCAGGGGGCAGATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC27970.18069245846399137No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27440.17726853987314706No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26630.17203575863053594No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGGGGGCAGATTGTGATGACTCAGTCTCCACTCTCC26560.1715835429675942No Hit
GTCCGGAGGAGTCTGTAGAGCTCGCATGCAGTAATAAATCCCAACATCCT26500.17119592954221563No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGCTCAGGCACAGATTTTACACTGAA26140.16887024898994402No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25270.1632498543219543No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25250.16312064984682811No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25210.1628622408965757No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24850.16053656034430408No Hit
GTAATACATGGCCGTGTCGTCAGAGGTCAGACTGTGCAGCTCCATGTAGGCTGTGCTGACGGACGTGTCGCGATT24580.15879229993010038No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGGGGGCAGATTGTGATGACTCAGTCTCCACT23710.15317190526211066No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA23100.14923116877076156No Hit
AGCCAGGGCAGTCTCCACAGGTCCTGATCTATTTGGGTTCTAATCGGGCC23060.14897275982050914No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22780.14716389716874234No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22500.1453550345169755No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT22290.14399838752815045No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22050.142447933826636No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21890.1414142980256264No Hit
GTGTAAAATCTGTGCCTGAGCCACTGCCACTGAACCTGTCAGGGACCCCG21120.1364399257332677No Hit
GAGTGGAGGCTGAGGATGTTGGGATTTATTACTGCATGCGAGCTCTACAG21060.1360523123078891No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGACCTGTGGAGACTGCCCTGGCTTCTGCACATA20950.135341687694695No Hit
GTGCAGAAGCCAGGGCAGTCTCCACAGGTCCTGATCTATTTGGGTTCTAA20570.1328868026672972No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20490.1323699847667924No Hit
CCCTAATAGTGTTGACTCAAACCTTGCACAGAAGTTTCAGGGCAGGGTCACCCTGAATCGCGACACGTCCGTCAG20270.13094873554040418No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19960.12894606617594806No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATCTGC19770.12771862366224915No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19770.12771862366224915No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19420.12545754534754064No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19360.12506993192216206No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19080.12326106927039525No Hit
ATGTATTACTGTGCGAGAGTGGCATTTTACCGTGGTTCGGAGAGTCATCACTTTGACTACTGGGGCCAGGGAACC19010.12280885360745354No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18530.11970794620442475No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18480.11938493501660924No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18260.11796368579022104No Hit
GGTATGTGCAGAAGCCAGGGCAGTCTCCACAGGTCCTGATCTATTTGGGT18250.11789908355265794No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18220.11770527683996863No Hit
CCACAGGTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA18050.11660703880139592No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18040.11654243656383281No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA17960.11602561866332801No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17920.11576720971307561No Hit
CAGTAATAAATCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGAGCCACTGCCA17890.11557340300038632No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17710.11441056272425051No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGGGGG17580.1135707336359302No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGGGGGCAGATTGTGATGACTCAGTCTC17420.11253709783492061No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17320.11189107545928961No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGATTTATTACTGCATGCGAGCTCTA17290.11169726874660031No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17250.1114388597963479No Hit
GTCTGGGGCTGAGGTGAAGACGCCTGGGGCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGA17240.1113742575587848No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17120.1105990307080276No Hit
GTATTACTGTGCGAGAGTGGCATTTTACCGTGGTTCGGAGAGTCATCACTTTGACTACTGGGGCCAGGGAACC17050.1101468150450859No Hit
GATTTATTACTGCATGCGAGCTCTACAGACTCCTCCGGACATGTGCTTTTTTGGCCAGGGGACCAAACTAGAGAT16970.10962999714458109No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTATGTGCAGAAGCCAGGGC16920.10930698595676559No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16900.1091777814816394No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16890.10911317924407629No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16880.10904857700651321No Hit
GTGGAGGCTGAGGATGTTGGGATTTATTACTGCATGCGAGCTCTACAGACTCCTCCGGACATGTGCTTTTTTGGC16770.10833795239331909No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16700.1078857367303774No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC16690.10782113449281429No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16580.10711050987962019No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG16450.10627068079129989No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16360.10568926065323199No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16120.10413880695171757No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16120.10413880695171757No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16060.10375119352633898No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGG16020.10349278457608659No Hit
TTATTACTGCATGCGAGCTCTACAGACTCCTCCGGACATGTGCTTTTTTG16010.10342818233852347No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16000.10336358010096036No Hit
GATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGC15830.10226534206238767No Hit
CTCCTGCATAGTAATGGATACAACTATTTGGATTGGTATGTGCAGAAGCCAGGGCAGTCTCCACAGGTCCTGAT15800.10207153534969839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG354.26553E-968.7859836
GTATCGC401.2323653E-860.1465735
AGTATCG650.058.11639834
GTACGAA200.00711910952.18645546
CGTATGC609.458745E-1151.90241241
ATCGCGT408.3820487E-751.64671737
ACCGATC1700.050.24876827
CGATCAG1750.048.86054629
CCGATCA1750.048.83363728
GGTATCA12350.047.205681
TCGTATG605.524271E-946.08259640
GACCGAT1900.044.9346926
TGGTATC3250.043.0096242
ACGAGAC2000.042.63001322
CCCACGA2000.042.6162419
ATGCCGT901.8189894E-1242.4453344
GTGGTAT3450.039.5281071
GTATCAA33150.039.18381
CACGAGA2100.038.9734921
TATCTCG1009.094947E-1237.8322936
Produced by FastQC (version 0.11.5)