FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547934
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72380.46759099548171945No Hit
CTACAGACTCCTCCGGACATGTGCTTTTTTGGCCAGGGGACCAAACTAGA59570.3848355291633881No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52270.3376758957423249No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49150.31751999762263766No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45420.29342336301160127No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43830.28315160723906835No Hit
AGTCTGTAGAGCTCGCATGCAGTAATAAATCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT42920.2772728036208262No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG41800.270037353013759No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41120.26564440085946817No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG37290.24090174387280078No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34280.2214564703663076No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGTTTGGTCCCCTGGCCAAAAAAGCACATGTCCGGAGGAGTCTGTAGAG33480.21628829136125957No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT32570.2104094877430175No Hit
GTAATGGATACAACTATTTGGATTGGTATGTGCAGAAGCCAGGGCAGTCTCCACAGGTCCTGATCTATTTGGGTT32350.20898823851662923No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32320.20879443180393994No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31990.20666255796435765No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGATTTATTACTGCATGCGAGCTCTACAGACT31520.20362625279889196No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31280.20207579909737755No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31260.20194659462225134No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30360.19613239324157233No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30180.1949695529654365No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29990.19374211045173761No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29860.1929022813634173No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTATGTGCAGAAGCCAGGGCAGTCTC29400.1899305784355147No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29150.18831552249643718No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT29100.18799251130862168No Hit
GTAATAAATCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGAGCCACTGCCA28310.18288893454113678No Hit
GTTCAGTGGCAGTGGCTCAGGCACAGATTTTACACTGAAAATCAGCAGAG27720.17907740252491386No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG27620.17843138014928286No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27590.17823757343659355No Hit
CCTCCGGACATGTGCTTTTTTGGCCAGGGGACCAAACTAGAGATCAAACG27450.17733314211071013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26950.17410303023255513No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26580.17171274744272041No Hit
GTCCGGAGGAGTCTGTAGAGCTCGCATGCAGTAATAAATCCCAACATCCT26150.16893485122750712No Hit
GTAATACATGGCCGTGTCGTCAGAGGTCAGACTGTGCAGCTCCATGTAGGCTGTGCTGACGGACGTGTCGCGATT26110.1686764422772547No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGGGGGCAGATTGTGATGA25040.161764002858003No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGTTTGGTCC25040.161764002858003No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGGGGGCAGATTGTGATGACTCAGTCTCCACTCTCC24320.15711264175345976No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24070.15549758581438225No Hit
GCTAATGCTCTGGGTCTCTGAATCCAGGGGGCAGATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC24000.15504537015144057No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA22740.14690548821848995No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGCTCAGGCACAGATTTTACACTGAA22330.14425679647840284No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22110.1428355472520146No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21990.1420603204012574No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21940.1417373092134419No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21850.14115588907537402No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21760.1405744689373061No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21600.1395408331362965No Hit
GTGTAAAATCTGTGCCTGAGCCACTGCCACTGAACCTGTCAGGGACCCCG21480.1387656062855393No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGGGGGCAGATTGTGATGACTCAGTCTCCACT20920.1351478809820057No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20580.1329514049048603No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20200.13049651987746247No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA19780.12778322589981225No Hit
GTGCAGAAGCCAGGGCAGTCTCCACAGGTCCTGATCTATTTGGGTTCTAA19340.12494072744703584No Hit
GAGTGGAGGCTGAGGATGTTGGGATTTATTACTGCATGCGAGCTCTACAG19260.12442390954653107No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGACCTGTGGAGACTGCCCTGGCTTCTGCACATA19140.12364868269577386No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19130.12358408045821075No Hit
AGCCAGGGCAGTCTCCACAGGTCCTGATCTATTTGGGTTCTAATCGGGCC19130.12358408045821075No Hit
CCCTAATAGTGTTGACTCAAACCTTGCACAGAAGTTTCAGGGCAGGGTCACCCTGAATCGCGACACGTCCGTCAG19100.12339027374552146No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT18910.12216283123182255No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18850.12177521780644394No Hit
ATGTATTACTGTGCGAGAGTGGCATTTTACCGTGGTTCGGAGAGTCATCACTTTGACTACTGGGGCCAGGGAACC18130.11712385670190073No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17950.11596101642576492No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATCTGC17880.11550880076282322No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17840.11525039181257082No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGGGGG17640.11395834706130882No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17410.11247249559735752No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17390.1123432911222313No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17340.11202027993441582No Hit
GTGGAGGCTGAGGATGTTGGGATTTATTACTGCATGCGAGCTCTACAGACTCCTCCGGACATGTGCTTTTTTGGC17310.11182647322172651No Hit
GTCTGGGGCTGAGGTGAAGACGCCTGGGGCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGA17150.1107928374207169No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17050.1101468150450859No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16950.10950079266945491No Hit
CAGTAATAAATCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGAGCCACTGCCA16920.10930698595676559No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16790.1084671568684453No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16770.10833795239331909No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16750.10820874791819289No Hit
GTATTACTGTGCGAGAGTGGCATTTTACCGTGGTTCGGAGAGTCATCACTTTGACTACTGGGGCCAGGGAACC16730.10807954344306668No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16670.10769193001768809No Hit
GGTATGTGCAGAAGCCAGGGCAGTCTCCACAGGTCCTGATCTATTTGGGT16640.10749812330499879No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC16480.1064644875039892No Hit
GTGCAGCTCCATGTAGGCTGTGCTGACGGACGTGTCGCGATTCAGGGTGA16450.10627068079129989No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16000.10336358010096036No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15960.10310517115070797No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15840.10232994429995078No Hit
GATTTATTACTGCATGCGAGCTCTACAGACTCCTCCGGACATGTGCTTTTTTGGCCAGGGGACCAAACTAGAGAT15820.10220073982482457No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG15600.10077949059843637No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15480.10000426374767916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13500.045.4165231
ATAAACG306.750871E-445.3769073
TGGTATC3450.042.4230272
AATGGTA4550.040.17029670
GTGGTAT3800.038.544291
AGCTTAG451.0840351E-437.82264315
GTATCAA33100.037.4583441
CGCAACA400.002451948434.9055748
TAAGACG400.002776617634.031585
AATACGT905.9390004E-934.02828210
ATACGTA905.9426384E-934.0260811
TATCAAC37050.033.2562032
TACGTAC951.0069925E-832.23523712
TCAACGC38700.032.0980074
ATCAACG38700.031.9221273
CAACGCA39300.031.6069375
AACGCAG39700.031.4599276
GTATAGG553.5107E-430.9658151
ACGTACC1001.6592821E-830.62446613
ACGGTTA450.004935833630.25909216
Produced by FastQC (version 0.11.5)