FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795924
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38690.4861016881008739No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37600.47240691322287054No Hit
GTATCGCACTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT34160.42918670626843775No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29680.37289992511847864No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29420.36963328156959707No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25960.32616179434217335No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25660.32239259024731004No Hit
GTGCGATACTGTTGGCAATGATAAGTTGCAAAATCAGCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC25120.3156080228765561No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24830.31196445891818814No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24330.30568245209341594No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22760.2859569506636312No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20800.26133148391052413No Hit
CACCATCACCAGCCTGCAGCCTGCTGATTTTGCAACTTATCATTGCCAACAGTATCGCACTTACCCGTGGACGTT20560.25831612063463344No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20250.25442127640327467No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19360.24323930425518017No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19330.2428623838456938No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGCTGATTTTGCAACTTATCATTGCCAACAGTATCGCACTTAC19030.2390931797508305No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19020.23896753961433503No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC19000.23871625934134416No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCG18910.23758549811288515No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18740.2354496157924626No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17540.22037279941300927No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17290.21723179600062317No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17190.21597539463566873No Hit
GTCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCAGA16980.2133369517692644No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16640.20906518712841932No Hit
GAGTGTTACTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTCTAAGACGT16290.20466778235107874No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16180.2032857408496289No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15370.19310888979349786No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15370.19310888979349786No Hit
GTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGCCTG15240.1914755680190571No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14710.18481664078479854No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14320.17991667546147622No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14200.1784089938235309No Hit
GTCAGAGTGTTACTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTCTAAGA13770.17300646795422678No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13430.16873470331338167No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13110.16471421894552746No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13060.16408601826305025No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13030.1637090978535639No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12800.16081937471416868No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12570.15792965157477346No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12310.15466300802589192No Hit
GTTACTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTCTAAGACGTCTA12270.15416044747991015No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12170.1529040461149557No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12090.15189892502299215No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12080.1517732848864967No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACGT11940.15001432297556047No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11900.1495117624295787No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11720.1472502399726607No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11590.14561691819821992No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11590.14561691819821992No Hit
CCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG11210.14084259301139307No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11200.1407169528748976No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11130.1398374719194295No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10810.13581698755157529No Hit
GTAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCAGAGTGTTACTAGCTGGTTGGCCTGGTATCAGCAGAAA10760.13518878686909805No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10520.13217342359320738No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10360.1301631814092803No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTCTAAGACGTCTACTGTAGAAAGTGGGGTCCCAT10280.12915806031731672No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10190.12802729908885774No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCAGAGTGTTACTAG10170.12777601881586687No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10150.12752473854287596No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10110.1270221779968942No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG10010.12576577663193975No Hit
GGTAAGTGCGATACTGTTGGCAATGATAAGTTGCAAAATCAGCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATT9990.12551449635894885No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9640.1211170915816083No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9630.12099145144511285No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACGTCCAGATGACCCAGTCTCCTTCCACC9600.12061453103562653No Hit
GTCCACGGGTAAGTGCGATACTGTTGGCAATGATAAGTTGCAAAATCAGC9590.12048889089913108No Hit
TCACCAGCCTGCAGCCTGCTGATTTTGCAACTTATCATTGCCAACAGTAT9540.11986069021665385No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9520.11960940994366297No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGCCTGCAGCCTGCTGATTTTG9460.11885556912469031No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9420.11835300857870852No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9410.11822736844221307No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG9410.11822736844221307No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9380.11785044803272675No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9300.11684532694076319No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9280.1165940466677723No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9090.11420688407435886No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9000.11307612284589985No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC8980.11282484257290896No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8880.11156844120795452No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8810.11068896025248642No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8770.11018639970650464No Hit
ACCTTGATGGGACCCCACTTTCTACAGTAGACGTCTTAGAGATTAGGAGCTTAGGGGCTTTCCCTGGTTTCTG8680.10905563847804566No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8570.10767359697659576No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8390.10541207451967775No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8250.10365311260874153No Hit
TCTCTAAGACGTCTACTGTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT8240.1035274724722461No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC8130.10214543097079622No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8110.10189415069780533No Hit
GCTAGTAACACTCTGACTGGCCCGGCAAGTGATGGTGATTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA8100.10176851056130987No Hit
CTACTGTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGG8050.10114030987883266No Hit
GTGTTACTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTCTAAGACGTCTA7990.10038646905986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT200.00813724450.449228
TTGGTCG305.909977E-446.61559346
TCGTATG1500.041.4344840
GTATCAA23650.039.9586751
CGTCGGA1203.4035293E-739.44396670
ATGCCGT1600.039.18603544
CGTATGC1600.038.91007641
ATCTCGT1600.038.56622737
CCGTCTT1550.038.407847
GTGGTAT2200.038.2167051
CGAGACC1750.036.5569723
ACGAGAC1750.036.53858622
CCCACGA1900.035.41185819
TAAGACA1850.034.84390631
AATGGTA3900.034.67601470
GGGTTAG400.002941815633.63071
TATCAAC28200.033.3921852
GACATCT1850.033.1528934
TATGCCG1900.032.92378643
TATACAC2050.032.8104443
Produced by FastQC (version 0.11.5)