FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795924
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39570.497158020112473No Hit
GTATCGCACTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT32940.41385860961599347No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31330.3936305476402269No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29830.3747845271659103No Hit
GTGCGATACTGTTGGCAATGATAAGTTGCAAAATCAGCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC28490.3579487488755208No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28480.35782310873902534No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24090.3026670888175253No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23960.3010337670430845No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23220.29173639694242165No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23030.2893492343490082No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22440.28193646629577696No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20410.2564315185872018No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20050.2519084736733658No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC19430.24411878521064825No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19380.24349058452817102No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18480.23218297224358106No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18470.2320573321070856No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18400.23117785115161749No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGCTGATTTTGCAACTTATCATTGCCAACAGTATCGCACTTAC17900.22489584432684528No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17830.22401636337137718No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17780.22338816268889997No Hit
CACCATCACCAGCCTGCAGCCTGCTGATTTTGCAACTTATCATTGCCAACAGTATCGCACTTACCCGTGGACGTT17570.2207497198224956No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCG16650.20919082726491475No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16590.20843698644594208No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15880.19951653675476552No Hit
GTCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCAGA15510.19486785170443408No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC15500.19474221156793864No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14970.1880832843336801No Hit
GAGTGTTACTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTCTAAGACGT14860.1867012428322302No Hit
GTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGCCTG14180.17815771355054No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14040.17639875163960378No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13820.173634668636704No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13810.17350902850020855No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13540.17011674481483158No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13480.1693629039958589No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13260.16659882099295914No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13250.1664731808564637No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12300.15453736788939648No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12100.1520245651594876No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12020.15101944406752402No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA11900.1495117624295787No Hit
GTCAGAGTGTTACTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTCTAAGA11850.1488835617471015No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11440.14373231615078827No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11360.14272719505882472No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11330.14235027464933836No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11250.14134515355737481No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11210.14084259301139307No Hit
GTTACTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTCTAAGACGTCTA11210.14084259301139307No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACGT11110.13958619164643862No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10850.13631954809755706No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10610.1333041848216664No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10560.13267598413918918No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10520.13217342359320738No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10410.1307913820917575No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10310.12953498072680306No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10260.12890678004432585No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10220.12840421949834407No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10030.12601705690493062No Hit
GGTAAGTGCGATACTGTTGGCAATGATAAGTTGCAAAATCAGCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATT9990.12551449635894885No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9830.12350425417502174No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9800.12312733376553539No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9710.12199657253707641No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCAGAGTGTTACTAG9650.12124273171810374No Hit
GTAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCAGAGTGTTACTAGCTGGTTGGCCTGGTATCAGCAGAAA9640.1211170915816083No Hit
CCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG9590.12048889089913108No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9500.11935812967067208No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9450.11872992898819484No Hit
GTCCACGGGTAAGTGCGATACTGTTGGCAATGATAAGTTGCAAAATCAGC9420.11835300857870852No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACGTCCAGATGACCCAGTCTCCTTCCACC9170.11521200516632242No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9160.11508636502982697No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9110.11445816434734976No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGCCTGCAGCCTGCTGATTTTG9100.1143325242108543No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9080.11408124393786341No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTCTAAGACGTCTACTGTAGAAAGTGGGGTCCCAT8990.11295048270940442No Hit
TCACCAGCCTGCAGCCTGCTGATTTTGCAACTTATCATTGCCAACAGTAT8920.1120710017539363No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8840.11106588066197275No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8770.11018639970650464No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8760.11006075957000919No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8750.10993511943351375No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT8690.10918127861454109No Hit
GTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGA8500.10679411602112765No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8450.10616591533865041No Hit
TCTCTAAGACGTCTACTGTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT8330.10465823370070508No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8220.10327619219925521No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8190.10289927178976886No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8180.10277363165327343No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8110.10189415069780533No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8070.10139159015182354No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8050.10114030987883266No Hit
ACCTTGATGGGACCCCACTTTCTACAGTAGACGTCTTAGAGATTAGGAGCTTAGGGGCTTTCCCTGGTTTCTG8040.10101466974233721No Hit
GTGTTACTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTCTAAGACGTCTA8010.10063774933285088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAATA100.008209839138.0532870
GTTATTA300.0023507669.0266470
TTAATAC252.954459E-453.6497083
AGGTCCG200.00762694351.28249435
GCGATTT200.007690884651.17431634
TACGTAT554.0984113E-450.20119570
TCATCGG307.2296493E-444.747458
GTATCAA26400.038.371971
TCGGTTT750.001886481936.8142170
GTGGTAT3150.036.205911
CGATTAA400.002932875733.65157724
CTCGCTA400.002942860133.62825423
CGGCGTA400.002968396833.5690312
CGTAAAT400.002970227533.56480815
TATCAAC29950.033.365232
ATCAACG30600.032.5448573
GTCGGTT550.003574006532.3193769
TCAACGC31150.032.185524
AACGCAG31400.032.042096
CAACGCA31400.031.9312695
Produced by FastQC (version 0.11.5)