FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359616
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGCCTCTCAGGCTGTTCATTTGCAGATACA49340.3628965825644888No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47710.3509079034080211No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG40390.29706917247222747No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA38200.28096168329881377No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37070.2726505130860478No Hit
GTATATTACTGTGCGAACCAGGTTATTCAAGTTGTGCCGGACGTGTTCTT35240.25919083035209944No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA32330.2377877282997552No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT31410.231021111843344No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT31070.2285204057616268No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30900.22727005272076822No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30150.2217537893052156No Hit
GAGTATTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC29890.2198414846544907No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAT28820.21197161551496893No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28580.2102064112219921No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28340.20844120692901524No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT25360.1865232536245528No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24970.18365479664846546No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT24760.18211024289211072No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT23980.17637332893993599No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC23450.17247516945961214No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22600.16622340425531915No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22070.1623252447749953No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21820.16048649030314444No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG21130.155411527960836No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21090.15511732724533986No Hit
GTATTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC20860.15342567313123706No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20710.15232242044812655No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGATCACCTTCGGCCA20640.15180756919600827No Hit
GTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCATCTACTTAGCCTGGTA20620.15166046883826023No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20590.1514398183016381No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20480.15063076633402372No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20380.14989526454528337No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGAGGCCAGTTGCTACGCTGCTGA19970.14687970721144794No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC19660.14459965166635286No Hit
GGACACGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC19640.1444525513086048No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19170.14099569290152514No Hit
GGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGG19060.14018664093391073No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT18750.13790658538881567No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18710.13761238467331952No Hit
GTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGAGGCCAGTTGCTACGCTG18240.13415552626623986No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC18120.13327292411975145No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17720.13033091696479007No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGTCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG17530.1289334635661834No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT17330.1274624599887027No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17160.1262121069478441No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG17120.12591790623234794No Hit
GTGTTGGAAGAACACGTCCGGCACAACTTGAATAACCTGGTTCGCACAGTAATATACGGCCGTGTCCTCGCCTCT16550.12172554603652795No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16490.12128424496328374No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA16490.12128424496328374No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16400.12062229335341743No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT16380.12047519299566936No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT15900.11694478440971569No Hit
CAGTAATATACGGCCGTGTCCTCGCCTCTCAGGCTGTTCATTTGCAGATA15550.11437052814912446No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT15360.1129730747505178No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC15330.11275242421389568No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCC15320.11267887403502165No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC15270.11231112314065148No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15010.11039881848992655No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15000.11032526831105252No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT14860.10929556580681604No Hit
CCCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGTTGGAAGAACA14700.10811876294483148No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14570.10716261061946902No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC14250.10480900489549991No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT14220.1045883543588778No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGG14220.1045883543588778No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14160.10414705328563359No Hit
GCGTAGCAACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCAT14030.10319090096027113No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC13920.10238184899265676No Hit
GCCTGGGGGGTCCCTGAGGCTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGTCATGAACTGGGTCCG13870.10201409809828658No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA13860.10194054791941255No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC13840.10179344756166446No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGACATAGTTGC13840.10179344756166446No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGATGTTGGAAT13740.10105794577292412No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT13710.100837295236302No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGATAGAGC13660.10046954434193182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA300.00249338468.01446570
TATCTCG950.050.73486736
TTGCACG306.104873E-446.31225246
GAGCCTA1550.044.30264331
ATCTCGT1100.043.85376737
GGTATCA10050.043.2746351
CGTATGC1400.041.9970541
TCGTATG1350.040.94799840
GTATCAA27200.039.28091
ACGAATT350.001376884239.25830532
GACCGGA1750.039.17159326
CACGAGA1850.038.87543521
GTAAGTT700.001446161838.86540670
TCTGGAG17050.038.69444770
AGACCGG1800.038.0792925
CTTATAC1800.038.0498771
AGCCTAT1650.037.4738432
GCCGTCT1300.037.4060546
ATGCCGT1400.037.14591644
ACGAGAC1850.037.0283122
Produced by FastQC (version 0.11.5)