FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359616
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47300.3478923460741857No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG42770.3145741150442478No Hit
GTAATATACGGCCGTGTCCTCGCCTCTCAGGCTGTTCATTTGCAGATACA42510.31266181039352287No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA33480.24624599887026924No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA33160.24389239314630012No Hit
GTATATTACTGTGCGAACCAGGTTATTCAAGTTGTGCCGGACGTGTTCTT31510.23175661363208436No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29850.21954728393899456No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29700.21844403125588402No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT29050.21366326962907173No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT28830.21204516569384296No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28670.2108683628318584No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26620.19579057616268122No Hit
GAGTATTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC25770.18953881095838826No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24550.18056568913575596No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24550.18056568913575596No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAT24280.17857983430615704No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT22010.16188394370175108No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGAGGCCAGTTGCTACGCTGCTGA21840.16063359066089247No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC21540.15842708529467145No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT21520.15827998493692338No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20630.15173401901713424No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20130.1480565100734325No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG19990.147026807569196No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19630.14437900112973073No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19510.14349639898324232No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19490.14334929862549425No Hit
GTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCATCTACTTAGCCTGGTA19410.142760897194502No Hit
GTATTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC19010.13981889003954057No Hit
GGACACGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC18930.1392304886085483No Hit
GTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGAGGCCAGTTGCTACGCTG18660.13724463377894935No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGATCACCTTCGGCCA18340.13489102805498024No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17980.13224322161551497No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17650.12981606571267182No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17630.12966896535492375No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC17500.12871281302956128No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17470.12849216249293918No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17290.12716825927320655No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGTCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG17100.12577080587459988No Hit
GGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGG16960.1247411033703634No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC16920.12444690265486726No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16770.12334364997175673No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16720.12297589907738656No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16670.12260814818301638No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16510.12143134532103184No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16240.11944549049143288No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16190.1190777395970627No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA16190.1190777395970627No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT16150.11878353888156656No Hit
GTGTTGGAAGAACACGTCCGGCACAACTTGAATAACCTGGTTCGCACAGTAATATACGGCCGTGTCCTCGCCTCT15970.11745963566183393No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15930.1171654349463378No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC15670.11525313029561288No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15600.11473827904349464No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT15480.11385567689700621No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT15090.11098721992091884No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC15030.11054591884767465No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC14830.10907491527019395No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC14770.10863361419694972No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT14240.10473545471662586No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCC14160.10414705328563359No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT14150.10407350310675956No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13850.10186699774053849No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGATGTTGGAAT13720.10091084541517606No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC13660.10046954434193182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT200.007046394552.32177450
GGTATCA10050.042.238551
TCTGGAG16650.042.22323670
GTATCAA25500.038.3954051
GTGAGCG7600.036.63454470
TATCAAC29000.033.5007062
ATCAACG29750.032.759083
GTGCTAG1155.0931703E-1132.7452431
TCAACGC30000.032.5916754
CAACGCA30500.032.169485
AACGCAG30400.032.162846
GACAGGT2250.031.951421
CCGCAGG10250.031.91672970
ATACCTT751.7926104E-631.9079346
AATGGTA4750.030.77301870
GTATACC450.00480158630.4299261
GGTATAT450.004833676430.3885125
TCAGGTC12550.029.95925712
CGCAGAG32450.029.8149248
CTCTTAT950.005767846529.30763470
Produced by FastQC (version 0.11.5)