FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317671
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61450.46635313367297304No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC57390.43554119351492143No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGG49710.3772565382405775No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46300.35137754416694306No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39850.3024275407138808No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38980.295824982108584No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36580.27761102733535153No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36460.2767003295966899No Hit
GCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCACTGAACCTTG36130.2741959108153705No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACCAACTATTTAAATTGGTATCAGCAGAAA35820.2718432749904946No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35220.26728978629718647No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34950.2652407163851978No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34790.264026452733649No Hit
ATATTACTGTCAACAGTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGA34520.26197738282166033No Hit
GCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCTGCTCTGG34070.2585622663016793No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33790.2564373049114688No Hit
AGTCAGGACATTACCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA33210.2520355991746043No Hit
GTAGTACACAGCCGTGTCTTCAGCTCTCAGGCTGTTCATTTGCAGAAACA32830.2491517230021758No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACCAA31940.24239738144043546No Hit
GATTTTAGTGTCACCATAAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGAAAATCTG30700.23298683814093202No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30600.23222792335871398No Hit
GTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT29660.2250941244058646No Hit
CCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC29420.22327272892854133No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCA29390.2230450544938759No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG29300.2223620311898797No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29130.22107187606010908No Hit
ACATATTACTGTCAACAGTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA28650.21742908510546258No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28550.2166701703232446No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28450.21591125554102655No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28230.2142416430201469No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCA28030.21272381345571084No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC27040.20521055711175246No Hit
CACTAAAATCTGTCCCAAATCCACTTCCACTGAACCTTGATGGGACCCCT26030.1975455178113505No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCT24990.18965280407628307No Hit
GGACATTACCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGTATC24540.186237687556302No Hit
GTGTACTACTGTGCGAAAGGTCAGAAGCAATGGCTGGTAGGAAGGTTTGA24520.18608590459985838No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24510.1860100131216366No Hit
CAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT24200.1836573772967607No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTC23910.18145652442832846No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23170.1758405550399151No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22940.1740950510408137No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22700.17227365556349042No Hit
GATCTACGATGTATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTG22530.1709835004337198No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21820.16559520547997186No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21420.1625595463510998No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCT20970.1591444298311187No Hit
GTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT20650.15671590252802103No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20630.15656411957157743No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG19590.14867140583651No Hit
TTTTCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTC19250.14609109557696875No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19100.14495272340364174No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18970.14396613418675833No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18770.14244830462232225No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18730.14214473870943506No Hit
CCATAAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAG18650.14153760688366065No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18550.14077869210144262No Hit
GTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGA18540.14070280062322083No Hit
TCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT18490.14032334323211182No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATACATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG18380.139488536971672No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18230.13835016479834494No Hit
ACCATAAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACA18110.13743946705968335No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17860.1355421801041383No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17780.13493504827836386No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17620.13372078462681503No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17600.13356900167037145No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17430.13227884654060082No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17250.13091279993260838No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA17020.12916729593350693No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAG17000.12901551297706332No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17000.12901551297706332No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGTATCCAATTTGGAAACAGGGGTCCCATCAAGGTT16860.1279530322819581No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16860.1279530322819581No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16710.12681466010863107No Hit
CATCAAGGTTCAGTGGAAGTGGATTTGGGACAGATTTTAGTGTCACCATAAGCAGCCTGCAGCCTGAAGATATTG16550.12560039645708224No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16420.1246138072401988No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16330.12393078393620259No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16300.12370310950153718No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16240.12324776063220638No Hit
GCCTGAAGATATTGCAACATATTACTGTCAACAGTATGAAAATCTGCCCC16020.12157814811132672No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15990.12135047367666132No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGGTA15790.11983264411222529No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATTGCTAC15660.11884605489534186No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15530.11785946567845842No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15490.11755589976557124No Hit
GGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCTGCTCTGGCTCT15220.11550682985358258No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14900.11307830255048491No Hit
ATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCACTGAACCTTGATGGGAC14900.11307830255048491No Hit
ACTGTCAACAGTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTG14870.1128506281158195No Hit
GTTCAGTGGAAGTGGATTTGGGACAGATTTTAGTGTCACCATAAGCAGCC14820.11247117072471048No Hit
CCCCTAAGCTCCTGATCTACGATGTATCCAATTTGGAAACAGGGGTCCCA14620.11095334116027446No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCA14500.11004264342161284No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14470.10981496898694744No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14370.1090560542047294No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14320.1086765968136204No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13950.10586861211941373No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13860.1051855888154175No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13780.10457845698964309No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13530.10268117003409805No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13490.10237760412121083No Hit
GCTCACCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC13240.1004803171656658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG2200.054.3849228
TCGACGT2150.054.08230629
CGTATGC1650.052.3463641
GATCGAC200.00758657451.35334431
ACTCGAC2350.050.89999827
CGAGACT2400.049.807423
ACGAGAC2400.049.7960522
TCTCCGA2300.048.96029311
TCGTATG1500.048.310540
CGACGTC2350.048.04071830
AGACTCG2500.047.8223725
GTTATTA702.847075E-547.43962570
GTGGTAT1900.046.6975481
CTTATAC2450.045.9644661
CACGAGA2600.045.96035421
GTCGTAT306.33966E-445.95886239
TGTCGTA306.3780724E-445.90255438
TCTGGAG9550.045.20424770
GACTCGA2650.045.1274426
GACGTCA2550.044.30484431
Produced by FastQC (version 0.11.5)