FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317671
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60200.4568666988952478No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGG49490.3755869257196979No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45630.3462928151260823No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44920.34090452017233436No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43470.3299002558301731No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35230.26736567777540826No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34710.26341932090787457No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACCAACTATTTAAATTGGTATCAGCAGAAA33430.25370521169548393No Hit
ATATTACTGTCAACAGTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGA31530.23928583083334154No Hit
GCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCACTGAACCTTG31420.23845102457290174No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31400.2382992416164581No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30960.23496001657469884No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30620.23237970631515759No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30620.23237970631515759No Hit
GCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCTGCTCTGG30500.23146900857649597No Hit
AGTCAGGACATTACCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA30270.22972350457739454No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACCAA30150.22881280683873287No Hit
GTAGTACACAGCCGTGTCTTCAGCTCTCAGGCTGTTCATTTGCAGAAACA29850.22653606249207883No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29280.22221024823343613No Hit
GTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT28640.2173531936272408No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28460.21598714701924837No Hit
CCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC28380.21538001519347394No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG28060.21295148789037627No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27330.20741140998018476No Hit
GATTTTAGTGTCACCATAAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGAAAATCTG27290.20710784406729751No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26670.20240257241754578No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCA25860.19625536268157986No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCA25670.1948134245953656No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25400.19276435468337696No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC25330.19223311433582435No Hit
ACATATTACTGTCAACAGTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA25130.1907152847713883No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24950.18934923816339586No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24080.18274667955809912No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23840.18092528408077585No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23510.1784208652994564No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCT23490.17826908234301278No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23450.17796551643012556No Hit
GGACATTACCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGTATC23400.17758605903901659No Hit
CAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT22740.17257722147637763No Hit
CACTAAAATCTGTCCCAAATCCACTTCCACTGAACCTTGATGGGACCCCT22200.16847908165240033No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTC21500.1631666781768742No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCT21370.16218008895999078No Hit
GTGTACTACTGTGCGAAAGGTCAGAAGCAATGGCTGGTAGGAAGGTTTGA21370.16218008895999078No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20840.15815784061423527No Hit
GTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT20670.15686768548446464No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20600.15633644513691203No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19820.15041690983561148No Hit
GATCTACGATGTATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTG19660.14920264618406262No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG19570.14851962288006643No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18480.14024745175389No Hit
TTTTCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTC18300.13888140514589756No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18210.13819838184190136No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18150.13774303297257054No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17970.13637698636457812No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17740.13463148236547667No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATACATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG17690.13425202497436764No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17550.1331895442792624No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17520.132961869844597No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17430.13227884654060082No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17320.131444040280161No Hit
GTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGA16900.1282565981948453No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAG16440.12476559019664242No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16250.12332365211042817No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16190.12286830324109736No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15830.1201362100251125No Hit
CCATAAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAG15800.11990853559044709No Hit
ACCATAAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACA15730.11937729524289448No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15660.11884605489534186No Hit
TCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT15360.11656931054868779No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15340.11641752759224419No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATTGCTAC15280.1159621787229134No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15240.11565861281002618No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA15080.11444434915847736No Hit
GGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCTGCTCTGGCTCT14990.11376132585448112No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14660.11125690707316166No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14620.11095334116027446No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14400.10928372863939483No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGGTA14310.10860070533539859No Hit
ATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCACTGAACCTTGATGGGAC14300.1085248138571768No Hit
CATCAAGGTTCAGTGGAAGTGGATTTGGGACAGATTTTAGTGTCACCATAAGCAGCCTGCAGCCTGAAGATATTG14210.10784179055318058No Hit
ACTGTCAACAGTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTG14190.10769000759673697No Hit
GTTCAGTGGAAGTGGATTTGGGACAGATTTTAGTGTCACCATAAGCAGCC14080.10685520133629715No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14030.10647574394518813No Hit
GCCTGAAGATATTGCAACATATTACTGTCAACAGTATGAAAATCTGCCCC13930.10571682916297011No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG13870.1052614802936393No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13810.1048061314243085No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13760.10442667403319948No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13710.10404721664209048No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCA13570.10298473594698525No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGTATCCAATTTGGAAACAGGGGTCCCATCAAGGTT13540.10275706151231985No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13400.10169458081721462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG9350.048.04377470
GTGGTAT2750.045.847721
GTATCAA27000.041.396041
TATCAAC30400.036.298552
ATCAACG31350.035.190583
TCAACGC31300.035.025234
CAACGCA31800.034.6886445
AACGCAG32450.034.098726
TATAAGA601.4666657E-534.0578962
ATAGGAC502.0107997E-434.050143
ACGCAGA33950.032.6924367
CGCAGAG34400.032.462728
AGAGTAC34250.032.30299411
CAGAGTA34750.031.8406210
CGTGTTC8250.031.40251452
GCAGAGT35400.031.3533369
AACTGTC9300.030.3722663
CGGGTAT450.00490933530.29208817
CGTCCAA450.00493309530.26219610
TTGGAAC9200.030.02653359
Produced by FastQC (version 0.11.5)