FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532435
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20120.37788650257777945No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14420.27083118127095324No Hit
CTCCTGTACAGCTTCTGGATTCACCTTTGGTGATTATTCTATGACCTGGTTCCGCCAGGCTCCAGGGAAGGGG14160.2659479560885366No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13660.2565571384300431No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13290.2496079333627579No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11270.21166903002244405No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGG10930.20528327401466845No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9590.1801158826899058No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC9460.1776742700986975No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9380.1761717392733385No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9350.17560829021382893No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC8970.16847126879337382No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT8180.15363377689295407No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8130.1526946951271047No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT8070.15156779700808548No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7770.14593330641298938No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC7250.1361668560481561No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT7070.13278616169109844No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC7060.13259834533792858No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC6990.13128363086573946No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG6860.12884201827453118No Hit
CCCTGAGACTCTCCTGTACAGCTTCTGGATTCACCTTTGGTGATTATTCT6770.12715167109600234No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6710.1260247729769831No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT6700.12583695662381325No Hit
GTACTAGAGATCGAGTGCAACCTGACTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGGAGTGCATCCG6610.12414660944528441No Hit
ACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGG6350.11926338426286776No Hit
ACAGAATACGCCGCGTCTGTGAAAGGCAGATTCACCCTCTCAAGAGATGA6330.11888775155652802No Hit
GTGTATTACTGTACTAGAGATCGAGTGCAACCTGACTTCTGGGGCCAGGG6260.11757303708433893No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT6230.11700958802482932No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC6200.11644613896531972No Hit
CTCTAGTACAGTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGGCGATGCTTTTGGAAT6190.11625832261214986No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6100.11456797543362099No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA6080.11419234272728127No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT6030.11325326096143191No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG5870.11024819931071399No Hit
ACCATGGAGTTTGGGCTTAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGG5810.10912130119169476No Hit
GCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAG5800.1089334848385249No Hit
TCTCTTGAGAGGGTGAATCTGCCTTTCACAGACGCGGCGTATTCTGTTGT5800.1089334848385249No Hit
GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTG5770.10837003577901527No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC5770.10837003577901527No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC5670.10649187224731657No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA5660.1063040558941467No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG5660.1063040558941467No Hit
CTCAAGAGATGATTCCAAAAGCATCGCCTATCTGCAAATGAACAGCCTGAAAACCGAGGACACAGCCGTGTATTA5600.10517715777512747No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC5390.1012330143585602No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG5390.1012330143585602No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5360.10066956529905058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG453.0120646E-853.69420240
GATTAGC200.007739274351.090939
CGTATGC552.5447662E-950.28893341
GGTATCA3450.044.4352461
TGGTATC759.840733E-1040.880432
TATACAC702.4429028E-838.9337353
ATGCCGT655.1995266E-737.37209744
AATGGTA3950.036.6503570
ACGAGAC754.4858098E-836.33815822
GTGGTAT853.3523975E-936.070961
GCCGTCT709.10135E-734.79254546
GTATGCC806.880691E-834.6132442
ATCTCGT501.8952723E-434.39005737
CACGAGA807.913695E-834.0670221
CTTATAC905.858965E-934.067021
ATTCACT400.002760865334.067022
AAACGCA500.002938165833.63575469
GTATCAA8400.032.850341
GTGAGCG3850.032.69765570
TGTTAGA2355.456968E-1232.14109870
Produced by FastQC (version 0.11.5)