FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532435
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20550.38596260576408387No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15380.28886155117526086No Hit
CTCCTGTACAGCTTCTGGATTCACCTTTGGTGATTATTCTATGACCTGGTTCCGCCAGGCTCCAGGGAAGGGG14970.28116108069529616No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12460.23401917604965863No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12120.22763342004188306No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10830.20340511048296978No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10650.20002441612591207No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10060.18894325128888972No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9210.17297886126945072No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGG9120.17128851409092188No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC9020.16941035055922318No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC8710.1635880436109572No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC8330.15645102219050214No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT8260.15513630771831305No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC7850.14743583723834836No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7820.14687238817883871No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC7240.13597903969498623No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC6970.13090799815939974No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6710.1260247729769831No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6630.12452224215162415No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT6620.12433442579845427No Hit
CCCTGAGACTCTCCTGTACAGCTTCTGGATTCACCTTTGGTGATTATTCT6610.12414660944528441No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA6590.12377097673894465No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC6580.12358316038577478No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC6540.12283189497309531No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6540.12283189497309531No Hit
GTACTAGAGATCGAGTGCAACCTGACTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGGAGTGCATCCG6400.12020246602871712No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG6300.11832430249701842No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT6260.11757303708433893No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG6110.11475579178679088No Hit
ACAGAATACGCCGCGTCTGTGAAAGGCAGATTCACCCTCTCAAGAGATGA6080.11419234272728127No Hit
GTACAGGAGAGTCTCAGGGACCGCCCTGGGTTTATCAAGCCTCCCCCAGA6050.11362889366777165No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT5970.11212636284241269No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT5860.11006038295754413No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA5820.10930911754486464No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG5820.10930911754486464No Hit
GTGTATTACTGTACTAGAGATCGAGTGCAACCTGACTTCTGGGGCCAGGG5660.1063040558941467No Hit
CTCTAGTACAGTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGGCGATGCTTTTGGAAT5650.10611623954097683No Hit
GGGCTGGAGTGGATAAGTTTCATTAGAAACAAAGCTTCTGGTGGGACAAC5630.1057406068346371No Hit
GGGTTCCCTGGCCCCAGAAGTCAGGTTGCACTCGATCTCTAGTACAGTAA5590.10498934142195761No Hit
ACCATGGAGTTTGGGCTTAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGG5570.10461370871561787No Hit
CTCAAGAGATGATTCCAAAAGCATCGCCTATCTGCAAATGAACAGCCTGAAAACCGAGGACACAGCCGTGTATTA5560.10442589236244801No Hit
GCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAG5490.1031111778902589No Hit
GGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGG5450.10235991247757943No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAGGTTGCA5440.10217209612440954No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT5380.10104519800539033No Hit
TCTCTTGAGAGGGTGAATCTGCCTTTCACAGACGCGGCGTATTCTGTTGT5350.10048174894588072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)