FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956546
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAAGTGGCCTCCGCTCACTTTCGGCGGAGGGACCAGGTTGGAGATCAAACGAACTGTGGCTGCACCAT32240.3370459967424463No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30970.32376906076654965No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28680.2998287588887518No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27360.2860291088980561No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26380.2757839142079942No Hit
CTTATAATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT25460.2661659763356911No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24700.25822072331074514No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22940.23982118998981755No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19640.20532206501307831No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19140.2000949248650875No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18900.19758589759405193No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17500.1829499051796777No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16430.17176382526297743No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16360.17103202564225872No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16180.16915025518898202No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT15870.16590942829722774No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15710.1642367434498707No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15220.1591141461048397No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14720.15388700595684893No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14530.1519006927006124No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14470.15127343588285352No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13440.14050552717799247No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13240.13841467111879618No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13240.13841467111879618No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATTATAAGTGG12860.13444204460632317No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12450.1301557896849707No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12150.12701950559617622No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12070.12618316317249773No Hit
CTGGTATCCCAGTCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT12020.12566044915769864No Hit
GTCCCAGACCCACTGCCACTGAACCTGACTGGGATACCAGTGGCCCTGGT12010.12555590635473882No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11910.12451047832514066No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11800.12336050749258269No Hit
CTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGGTGCATCCACCAGGGCCA11500.1202242234037882No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11480.12001513779786858No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11420.11938788098010969No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11110.1161470540883554No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACCTGGTCCCTCCGCCGAAAGTGAGCGGAGGCCACTTATAATACTG11090.11593796848243577No Hit
GTCTGCATACTTTGTGCTAGTTCCATCATAAGTAATAAGGGAGACCCACT11010.11510162605875723No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11000.11499708325579741No Hit
GTTTATTACTGTCAGCAGTATTATAAGTGGCCTCCGCTCACTTTCGGCGG10940.11436982643803853No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10760.11248805598476183No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT10580.11060628553148516No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10490.10966540030484682No Hit
GTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACGACAGCA10230.10694728742789161No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10210.10673820182197198No Hit
CTCCTGCAGGGCCAGTGAGACTGTTCGTGGCCTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT10050.10506551697461491No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9880.10328828932429804No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9870.10318374652133822No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTGAGACTGTTCGTGG9850.10297466091541861No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9800.10245194690061953No Hit
GTAATATAAGGCGGTGTCTTCACTTCTCAGACTATTCATTTGCAGATACA9600.10036109084142321No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9590.1002565480384634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.038.8836561
GTATCAA25650.038.3094941
GTATAGA502.1978753E-433.537151
ATCAACG30950.031.6325953
TCAACGC31350.031.4428864
TATCAAC31650.031.0405392
CAACGCA32000.030.7058495
CGCAGAG32300.030.2398828
AACGCAG32400.030.027246
ACGCAGA33350.029.1749427
AGAGTAC33100.029.01564811
GCAGAGT34000.028.7339029
GTAATAG606.4075337E-427.9403213
GACAGGT2050.027.8112951
CCTAGGC855.528742E-627.6116123
ATTCGGA500.00881844926.86765117
CAGAGTA36000.026.86343210
GTATATA757.472058E-526.829721
CGGGACT650.001012721225.8342817
GTAGTTT2158.9845344E-725.81221470
Produced by FastQC (version 0.11.5)