FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375513
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATAGTGCATCCAGTTTGCAAAGTGGGGTCCCATCTAGATTCGCCGGGAGTGGATCTGGAACGGAATTCA53040.3856015900976581No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43430.31573674694459447No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42800.3111566375599503No Hit
CTATAGATCAGGGACCTAGGGGCTTTCCCTGGTCTATGTTGAACCCAGGC40130.29174569778693477No Hit
GTACTATTGTCAGAAGTCTGATAGTTCGCCTCCATTCACTTTCGGCCCTGGGACCAGGGTGGACATCAAACGAA37690.2740068614400591No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35470.25786742837036075No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30210.2196271500160304No Hit
CAATAGTACATTGCAAAATCCTCGGGCTGCAGGTTGTTGATAGTGAGAGT28660.20835862692682658No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27550.2002889103919774No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27410.1992711083065009No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26950.1959269014542211No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26950.1959269014542211No Hit
ACATAGACCAGGGAAAGCCCCTAGGTCCCTGATCTATAGTGCATCCAGTTTGCAAAGTGGGGTCCCATCTAGATT26710.19418209787911855No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26510.19272809489986645No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25190.18313167523680257No Hit
GTCTGGAGGTCTGGGGCCCGGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG24990.18167767225755047No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC24800.18029636942726096No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24270.17644326153224288No Hit
GTCCCCATCCTCTGTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGTCGGGCGAGTGAGGGCATCAACAA24100.1752073589998786No Hit
GGTCTATGTTGAACCCAGGCTAAGTAGTTGTTGATGCCCTCACTCGCCCG23230.16888244604013192No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23080.16779194380569284No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23070.16771924365673027No Hit
CTCCAGGGAGGGGGCTGGAGTGGGTCGCCGCTATCAGTCGTAGGGGTTTC22960.1669195420181416No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21610.1571050219081899No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21110.15347001446005962No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20890.1518706111828823No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCAGGGCCGAAAGTGAATGGAGGCGAACTATCAGACTT20730.15070740879948064No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20610.14983500701192937No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20500.1490353053733407No Hit
ATGTTGAACCCAGGCTAAGTAGTTGTTGATGCCCTCACTCGCCCGACAAG20410.14838100403267726No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT20400.14830830388371466No Hit
CCTCCAGACCGTACGGGTAGCCCCAGGACCCCTTATAGCACTTGGAATAA20370.14809020343682686No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20290.14750860224512602No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20090.1460545992658739No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20080.14598189911691128No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20010.14547299807417305No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19850.14430979569077135No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19190.13951158585923942No Hit
GTAATATTCGCCCGTGTCTTCGGTTCTCAAATCGTTCAGTTGCAAAAACA18960.13783948243309949No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18860.13711248094347345No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18190.13224157096297892No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCC17860.12984246604721292No Hit
CTATCAGACTTCTGACAATAGTACATTGCAAAATCCTCGGGCTGCAGGTTGTTGATAGTGAGAGTGAATTCCGTT17770.12918816470654948No Hit
GTCGGAGACAGAGTCACCATCACTTGTCGGGCGAGTGAGGGCATCAACAACTACTTAGCCTGGGTTCAACATAGA17630.128170362621073No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT17300.12577125770530703No Hit
GTCTGATAGTTCGCCTCCATTCACTTTCGGCCCTGGGACCAGGGTGGACATCAAACGAACTGTGGCTGCACCAT17230.1252623566625688No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17130.12453535517294276No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16950.12322675249161585No Hit
CCCTGGTCCCAGGGCCGAAAGTGAATGGAGGCGAACTATCAGACTTCTGA16890.12279055159784022No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16600.12068224727792468No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16500.11995524578829864No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16410.11930094444763517No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16330.11871934325593433No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16080.11690183953186921No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15880.1154478365526171No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15760.11457543476506583No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15730.11435733431817803No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15620.11355763267958936No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15360.11166742880656164No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14960.10875942284805742No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14940.10861402255013221No Hit
GAATTCACTCTCACTATCAACAACCTGCAGCCCGAGGATTTTGCAATGTACTATTGTCAGAAGTCTGATAGTTCG14730.1070873194219175No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14570.10592411703851581No Hit
GACTACGCAGACTCCGTGAAGGGCCGGTTCACCATATTCAAAGACAATTC14530.1056333164426654No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14480.10526981569785236No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14280.10381581271860026No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14240.10352501212274985No Hit
CACTATCAACAACCTGCAGCCCGAGGATTTTGCAATGTACTATTGTCAGAAGTCTGATAGTTCGCCTCCATTCA14200.1032342115268994No Hit
CAGTAATATTCGCCCGTGTCTTCGGTTCTCAAATCGTTCAGTTGCAAAAA14050.10214370929246033No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14030.10199830899453512No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13990.10170750839868471No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13950.1014167078028343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6400.044.004371
CTTATAC1400.040.711531
CGTATGC1200.040.13796241
GTATCAA16450.038.5205461
TCGTATG1250.038.4335140
TATACAC1700.037.471513
TTATACA1750.036.4008942
ACGTAGA1600.035.726527
CACGAGA1600.035.63295421
TGGTATC1700.035.4993252
GTGGTAT1800.035.389761
GTATGCC1450.033.27721842
TTTGAAT9450.032.8318370
GAGACGT1850.032.64949424
GACGTAG1750.032.64161726
AGACGTA1750.032.6190325
ATCTCCG1750.032.56685610
ATCTCGT1400.031.63262637
ATCAACG20450.031.4778043
CAACGCA20450.031.149915
Produced by FastQC (version 0.11.5)