FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375513
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATAGTGCATCCAGTTTGCAAAGTGGGGTCCCATCTAGATTCGCCGGGAGTGGATCTGGAACGGAATTCA51840.37687757222214546No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41500.3017056181948117No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37610.2734252602483582No Hit
CTATAGATCAGGGACCTAGGGGCTTTCCCTGGTCTATGTTGAACCCAGGC37430.27211665756703135No Hit
GTACTATTGTCAGAAGTCTGATAGTTCGCCTCCATTCACTTTCGGCCCTGGGACCAGGGTGGACATCAAACGAA36890.26819084952305067No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34190.24856180930314725No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31080.22595206297577702No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27650.20101591188160345No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26700.19410939773015595No Hit
CAATAGTACATTGCAAAATCCTCGGGCTGCAGGTTGTTGATAGTGAGAGT25910.18836608596211013No Hit
GTCTGGAGGTCTGGGGCCCGGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG25370.18444027791812945No Hit
GTCCCCATCCTCTGTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGTCGGGCGAGTGAGGGCATCAACAA25230.18342247583265298No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25110.1825500740451017No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24990.18167767225755047No Hit
ACATAGACCAGGGAAAGCCCCTAGGTCCCTGATCTATAGTGCATCCAGTTTGCAAAGTGGGGTCCCATCTAGATT24320.17680676227705588No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24020.17462575780817774No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23930.1739714564675143No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23740.1725901536372248No Hit
GGTCTATGTTGAACCCAGGCTAAGTAGTTGTTGATGCCCTCACTCGCCCG23480.17069994976419706No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23400.17011834857249622No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22100.16066732920735755No Hit
CTCCAGGGAGGGGGCTGGAGTGGGTCGCCGCTATCAGTCGTAGGGGTTTC21890.15914062607914284No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21570.15681422131233946No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21210.15419701594968568No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21050.15303381356628398No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21050.15303381356628398No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20680.1503439080546676No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20480.1488899050754155No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20250.14721780164927556No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCAGGGCCGAAAGTGAATGGAGGCGAACTATCAGACTT20090.1460545992658739No Hit
ATGTTGAACCCAGGCTAAGTAGTTGTTGATGCCCTCACTCGCCCGACAAG19840.14423709554180877No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19200.13958428600820202No Hit
CCTCCAGACCGTACGGGTAGCCCCAGGACCCCTTATAGCACTTGGAATAA19200.13958428600820202No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19180.13943888571027682No Hit
GTCGGAGACAGAGTCACCATCACTTGTCGGGCGAGTGAGGGCATCAACAACTACTTAGCCTGGGTTCAACATAGA19160.1392934854123516No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18770.13645817960281No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT18320.13318667289949276No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18110.13165996977127806No Hit
GTAATATTCGCCCGTGTCTTCGGTTCTCAAATCGTTCAGTTGCAAAAACA18110.13165996977127806No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17990.13078756798372682No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17930.13035136708995115No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17750.12904276440862428No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17610.1280249623231478No Hit
GTCTGATAGTTCGCCTCCATTCACTTTCGGCCCTGGGACCAGGGTGGACATCAAACGAACTGTGGCTGCACCAT17580.12780686187625998No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT17430.1267163596418209No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17140.12460805532190536No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16860.1225724511509524No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16520.12010064608622383No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16500.11995524578829864No Hit
CTATCAGACTTCTGACAATAGTACATTGCAAAATCCTCGGGCTGCAGGTTGTTGATAGTGAGAGTGAATTCCGTT16430.11944634474556039No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCC16420.11937364459659779No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16410.11930094444763517No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16330.11871934325593433No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16220.11791964161734568No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16060.11675643923394399No Hit
CCCTGGTCCCAGGGCCGAAAGTGAATGGAGGCGAACTATCAGACTTCTGA15990.11624753819120576No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG15860.11530243625469189No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15570.11319413193477633No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15440.11224902999826247No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15360.11166742880656164No Hit
GGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15140.1100680255293843No Hit
GAATTCACTCTCACTATCAACAACCTGCAGCCCGAGGATTTTGCAATGTACTATTGTCAGAAGTCTGATAGTTCG14640.10643301808125405No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14630.10636031793229143No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14630.10636031793229143No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14550.10577871674059061No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14450.10505171525096454No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14340.10425201361237589No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14180.10308881122897422No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14080.10236180973934815No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC14060.10221640944142293No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14050.10214370929246033No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13790.1002535054194326No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATGCG200.007841160550.92644535
GTGGTAT2550.045.9249421
TGGTATC2650.044.1790732
GGTATCA6200.037.776971
GTATCAA15000.036.5824971
GACAGGT1101.4697434E-930.4178161
TTTGAAT9800.030.11878870
TCAACGC19350.028.0035254
ATCAACG19400.027.931353
GTGTATA606.486394E-427.8831
AACGCAG19600.027.823066
CAACGCA19500.027.7901335
AGAGTAC20000.027.29935511
TCACTCG25600.026.9029870
CAGAGTA20350.026.8278810
CGCAGAG20600.026.3320398
GCCGACG1359.690687E-426.02858270
GCAGAGT21550.025.9509539
GAGTTTA5450.025.1714421
CTCTAGG700.001594410223.8997151
Produced by FastQC (version 0.11.5)