FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004687043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522227
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21720.4159110884730204No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20460.39178364963895007No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG17660.3381671188965718No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16990.3253374490403598No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAAGCGACTCTGGACATCAAGAGATCTCTAAATTGGTATCGACAGAAA14130.2705719926392163No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13800.2642529015160074No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13510.2586997608319754No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13300.254678521026297No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13030.24950835556185338No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12570.2406999255113198No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12490.23916802463296613No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12480.23897653702317193No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12410.23763612375461246No Hit
ATATTATTGTCAACAGTATGAATTAATCCCCCGGACGTTCGGCCTAGGGA12110.2318914954607862No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11780.22557240433757733No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11550.22116818931231055No Hit
CAATAATATGTTGCAAAATCCTCAGGCTGCAGGCCGTTGATGGTGAAACTAAAATCTGTTCCAGATCCAGTTCCA11060.21178529643239435No Hit
GTATGAATTAATCCCCCGGACGTTCGGCCTAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT11050.21159380882260015No Hit
ACTCTGGACATCAAGAGATCTCTAAATTGGTATCGACAGAAACCAGGGAA10870.2081470318463044No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10730.20546620530918547No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10700.20489174247980282No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10530.2016364531133013No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC10040.1922535602333851No Hit
GTTCAGTGGAACTGGATCTGGAACAGATTTTAGTTTCACCATCAACGGCC10000.19148760979420826No Hit
GCCTAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTC9900.18957273369626618No Hit
AATTCATACTGTTGACAATAATATGTTGCAAAATCCTCAGGCTGCAGGCCGTTGATGGTGAAACTAAAATCTGTT9820.18804083281791253No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9250.17712603905964264No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9230.17674306384005423No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8680.16621124530137277No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTAGGCCGAACGTC8530.16333893115445966No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8440.16161554266631178No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC8150.15606240198227972No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8110.1552964515431029No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGGCAGATGTGACAT8070.15453050110392608No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAAGCGACTCTGG7910.15146669934721874No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT7820.1497433108590709No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7810.14955182324927666No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7720.14782843476112878No Hit
GTTCAGGGGCTTTCCCTGGTTTCTGTCGATACCAATTTAGAGATCTCTTG7650.14648802149256931No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7640.1462965338827751No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT7440.14246678168689095No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7430.14227529407709674No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7420.14208380646730254No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7310.13997744275956622No Hit
CCACTGAACCGTGGTGGGACCCCTGTCTCCAAATTGGAAGCATCGTAGAT7290.13959446753997784No Hit
GAACCGTGGTGGGACCCCTGTCTCCAAATTGGAAGCATCGTAGATCAGGAGTTCAGGGGCTTTCCCTGGTTT7120.1363391781734763No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7080.13557322773429947No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6820.13059454987965005No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6780.1298285994404732No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6750.1292541366110906No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6740.12906264900129638No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG6720.12867967378170794No Hit
CCACGGTTCAGTGGAACTGGATCTGGAACAGATTTTAGTTTCACCATCAA6620.12676479768376586No Hit
GAATTAATCCCCCGGACGTTCGGCCTAGGGACCAGGGTGGAGATCAAACG6600.12638182246417745No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6560.12561587202500063No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6500.12446694636623537No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6440.12331802070747012No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6330.12121165699973382No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6280.1202542189507628No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT6220.11910529329199754No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6210.11891380568220333No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6180.1183393428528207No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6160.11795636763323228No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6120.11719041719405546No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGGCAGATGTGACATCCAGATGACCCAGTCTCCATC6090.11661595436467283No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGGCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC6030.1154670287059076No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5950.11393512782755391No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5780.11067983846105238No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5720.10953091280228712No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC5700.10914793758269872No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5640.10799901192393346No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5550.10627562343578559No Hit
GATTTTAGTTTCACCATCAACGGCCTGCAGCCTGAGGATTTTGCAACATATTATTGTCAACAGTATGAATTAATC5500.10531818538681455No Hit
GTTGACAATAATATGTTGCAAAATCCTCAGGCTGCAGGCCGTTGATGGTG5490.10512669777702034No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5460.10455223494763773No Hit
GTGGTGGGACCCCTGTCTCCAAATTGGAAGCATCGTAGATCAGGAGTTCA5450.10436074733784349No Hit
GGATTAATTCATACTGTTGACAATAATATGTTGCAAAATCCTCAGGCTGCAGGCCGTTGATGGTGAAACTAAAAT5360.10263735884969563No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT5360.10263735884969563No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5310.10167992080072459No Hit
GATCAGGAGTTCAGGGGCTTTCCCTGGTTTCTGTCGATACCAATTTAGAG5300.10148843319093039No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT5270.10091397036154776No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5260.10072248275175355No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGCCACCTGAGAGCCAGAG5250.10053099514195933No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG5250.10053099514195933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)