FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522227
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21570.41303877432610725No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG16780.32131620923468146No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16390.31384819245270734No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15790.30235893586505486No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15620.2991036464985533No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15550.2977632332299939No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAAGCGACTCTGGACATCAAGAGATCTCTAAATTGGTATCGACAGAAA12630.24184885117008503No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12500.23935951224276034No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12450.23840207419378928No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12070.2311255450216094No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12070.2311255450216094No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11980.2294021565334615No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11400.21829587516539745No Hit
ATATTATTGTCAACAGTATGAATTAATCCCCCGGACGTTCGGCCTAGGGA11080.21216827165198276No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10510.2012534778937129No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10120.19378546111173878No Hit
ACTCTGGACATCAAGAGATCTCTAAATTGGTATCGACAGAAACCAGGGAA10100.19340248589215034No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT10000.19148760979420826No Hit
CAATAATATGTTGCAAAATCCTCAGGCTGCAGGCCGTTGATGGTGAAACTAAAATCTGTTCCAGATCCAGTTCCA9990.19129612218441405No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9630.18440256823182255No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9510.18210471691429206No Hit
GTATGAATTAATCCCCCGGACGTTCGGCCTAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT9470.1813387664751152No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9230.17674306384005423No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC9140.17501967535190635No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9020.17272182403437586No Hit
GTTCAGTGGAACTGGATCTGGAACAGATTTTAGTTTCACCATCAACGGCC8970.17176438598540483No Hit
AATTCATACTGTTGACAATAATATGTTGCAAAATCCTCAGGCTGCAGGCCGTTGATGGTGAAACTAAAATCTGTT8870.16984950988746275No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8860.16965802227766852No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTAGGCCGAACGTC8750.16755165856993223No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8740.16736017096013803No Hit
GCCTAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTC8700.1665942205209612No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8450.161807030276106No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8120.1554879391528971No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7950.15223264978639556No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG7780.148977360419894No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAAGCGACTCTGG7610.1457220710533925No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT7480.14323273212606777No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7460.14284975690647936No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC7350.14074339319874307No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT7350.14074339319874307No Hit
GAACCGTGGTGGGACCCCTGTCTCCAAATTGGAAGCATCGTAGATCAGGAGTTCAGGGGCTTTCCCTGGTTT7350.14074339319874307No Hit
CCACTGAACCGTGGTGGGACCCCTGTCTCCAAATTGGAAGCATCGTAGAT7340.14055190558894887No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7320.14016893036936046No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGGCAGATGTGACAT7060.13519025251471103No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6870.13155198792862108No Hit
GTTCAGGGGCTTTCCCTGGTTTCTGTCGATACCAATTTAGAGATCTCTTG6810.13040306226985585No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6790.1300200870502674No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6590.12619033485438325No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6370.12197760743891067No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6360.12178611982911645No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6230.11929678090179174No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6190.11853083046261491No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6160.11795636763323228No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGGCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC6150.11776488002343809No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6100.11680744197446705No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6020.11527554109611338No Hit
GATTTTAGTTTCACCATCAACGGCCTGCAGCCTGAGGATTTTGCAACATATTATTGTCAACAGTATGAATTAATC6010.11508405348631917No Hit
CCACGGTTCAGTGGAACTGGATCTGGAACAGATTTTAGTTTCACCATCAA5990.11470107826673075No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5980.11450959065693654No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC5720.10953091280228712No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGGCAGATGTGACATCCAGATGACCCAGTCTCCATC5680.10876496236311028No Hit
GAATTAATCCCCCGGACGTTCGGCCTAGGGACCAGGGTGGAGATCAAACG5580.1068500862651682No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5560.10646711104557981No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGCCACCTGAGAGCCAGAG5560.10646711104557981No Hit
GTGGTGGGACCCCTGTCTCCAAATTGGAAGCATCGTAGATCAGGAGTTCA5530.10589264821619718No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5510.10550967299660875No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5500.10531818538681455No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5450.10436074733784349No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC5300.10148843319093039No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5230.10014801992237092No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT200.007741573351.0869328
GGTATCA5250.045.314791
GTATCAA14650.039.6697771
TATCAAC16200.036.0736242
TCAACGC16700.035.1599434
GTGGTAT2450.034.6796881
GGCGCTC501.9504305E-434.22240434
CAACGCA17250.034.035645
CTTAACG400.00279713433.976322
ATGTCGG400.002812948333.9372756
GAATCGA502.0487147E-433.937276
AATCGAA502.0487147E-433.937277
AACGCAG17400.033.742236
ACGCAGA18100.032.624787
CGCAGAG18200.032.2590568
AGAGTAC18650.030.92885411
ACGGGAT553.5679617E-430.8757216
CAGAGTA18750.030.76684810
GCAGAGT19450.030.0084929
ATCTATG500.00837696527.1498177
Produced by FastQC (version 0.11.5)