FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204840
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56600.46977191992297734No Hit
GTCATAGTGTGGCTAGCGACTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTCCCAGGCCCCTCATCTAT46140.3829554131668936No Hit
GTCTGGGACAGACTTCAGTCTCACCATCGCCAGACTGGAGCCTGAAGATT45350.3763985259453537No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45240.37548554164868364No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATACCA39280.3260183924836493No Hit
GTCCCAGACCCACCGGCACTGAACCTGTCTGGGATGTCAGTGGCCCTATT34580.28700906344410876No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33690.27962219049832343No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33570.2786262076292288No Hit
GTCTATTACTGTCAGTTGTATGGTGAGTCATCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATCAGGCGAA31660.26277348029613895No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30780.2554696059227782No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC30590.2538926330467116No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30510.25322864446731513No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAACGAGCAACCCTCTCCTGCAGGGCCAGTCATAGTGTGGCTAG29550.24526078151455794No Hit
CCATACAACTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGC29080.2413598486106039No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28130.23347498423027124No Hit
CTATTACTGTCAGTTGTATGGTGAGTCATCGTGGACATTCGGCCAAGGGA27950.23198100992662926No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26720.22177218551840908No Hit
GTTGTATGGTGAGTCATCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATCAGGCGAACTGTGGCTGCACCAT26720.22177218551840908No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT25710.21338932970352908No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGCGATGGTGAGACTGAAGTCTGTCCCAGACCCACCGGCA25540.21197835397231168No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24180.2006905481225723No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24130.20027555526044954No Hit
GTGCAGCCACAGTTCGCCTGATTTCCACCTTGGTCCCTTGGCCGAATGTC23790.19745360379801466No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23610.1959596294943727No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23280.1932206766043624No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAACGAGCAACCCTCTCCTGC23200.19255668802496598No Hit
CACTATGACTGGCCCTGCAGGAGAGGGTTGCTCGTTCCCCTGGAGACAAA23100.19172670230072042No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22700.18840675940373824No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21910.18184987218219847No Hit
ATCTTACATCCAATAGGGCCACTGACATCCCAGACAGGTTCAGTGCCGGTGGGTCTGGGACAGACTTCAGTCTCA21770.1806878921682547No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21510.1785299292852163No Hit
ACCCTCTCCTGCAGGGCCAGTCATAGTGTGGCTAGCGACTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCT21250.1763719664021779No Hit
CAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGCGATGGTGAGACTGAAGTCTGTCCCAGACCCACCGGCA20980.1741310049467149No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATAC20070.16657813485608047No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19470.16159822051060724No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19310.16027024335181436No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19300.16018724477938978No Hit
GTAATAAGTGGCCGTGTCTTCAGATGTCAGGCCGCTCAGGTCCATGTAAA16680.13844161880415656No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT16670.13835862023173204No Hit
GTCCACGATGACTCACCATACAACTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGCGATGGTGAGA16570.13752863450748648No Hit
CATCTATCTTACATCCAATAGGGCCACTGACATCCCAGACAGGTTCAGTG16350.1357026659141463No Hit
CTCCTGCAGGGCCAGTCATAGTGTGGCTAGCGACTATTTAGCCTGGTACC16340.13561966734172173No Hit
GTCTTTGTCTCCAGGGGAACGAGCAACCCTCTCCTGCAGGGCCAGTCATA16330.13553666876929718No Hit
GTTCAGTGCCGGTGGGTCTGGGACAGACTTCAGTCTCACCATCGCCAGAC16280.1351216759071744No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG16260.13495567876232528No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16240.13478968161747618No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16220.13462368447262707No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16090.13354470303110788No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16030.13304671159656056No Hit
ATCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATCAGGCGAACTGTGG15950.1323827230171641No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15640.1298097672720029No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15520.12881378440290828No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCCTGATTTCCACCTTGGTCC15450.1282327943959364No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15370.12756880581653995No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15260.12665582151986987No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAAGTCTCCTGCA14790.12275488861591581No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14690.12192490289167027No Hit
CAATAGGGCCACTGACATCCCAGACAGGTTCAGTGCCGGTGGGTCTGGGA14600.12117791573984928No Hit
GACTTCAGTCTCACCATCGCCAGACTGGAGCCTGAAGATTTTGCAGTCTA14400.11951794429135819No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14370.11926894857408454No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT13590.1127950599249693No Hit
GCACTGAACCTGTCTGGGATGTCAGTGGCCCTATTGGATGTAAGATAGAT13580.11271206135254473No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA13500.11204807277314831No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT13470.11179907705587465No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC13330.11063709704193089No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13220.10972411274526078No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13110.1088111284485907No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12960.10756614986222238No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12820.1064041698482786No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12640.10491019554463663No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT12490.10366521695826833No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12490.10366521695826833No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12460.10341622124099466No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12430.103167225523721No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12290.10200524550977724No Hit
GCCCTATTGGATGTAAGATAGATGAGGGGCCTGGGAGGCTGGCCAGGTTTCTGCTGGTACCAGGCTAAATAGTCG12170.10100926264068259No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA12150.10084326549583349No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)