FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204840
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56280.46711596560539165No Hit
GTCTGGGACAGACTTCAGTCTCACCATCGCCAGACTGGAGCCTGAAGATT45530.37789250024899573No Hit
GTCATAGTGTGGCTAGCGACTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTCCCAGGCCCCTCATCTAT45190.3750705487865609No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39820.33050031539457525No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATACCA37680.3127386208957206No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34170.28360612197470203No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33690.27962219049832343No Hit
GTCCCAGACCCACCGGCACTGAACCTGTCTGGGATGTCAGTGGCCCTATT33230.27580425616679394No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32940.2733972975664819No Hit
GTCTATTACTGTCAGTTGTATGGTGAGTCATCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATCAGGCGAA31760.2636034660203844No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC29980.2488297201288138No Hit
GTTGTATGGTGAGTCATCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATCAGGCGAACTGTGGCTGCACCAT28620.23754191427907442No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAACGAGCAACCCTCTCCTGCAGGGCCAGTCATAGTGTGGCTAG28550.2369609242721025No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27930.23181501278178016No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27210.22583911556721226No Hit
CCATACAACTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGC25840.2144683111450483No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT25690.21322333255868No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25230.20940539822715049No Hit
GTGCAGCCACAGTTCGCCTGATTTCCACCTTGGTCCCTTGGCCGAATGTC24910.20674944390956473No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGCGATGGTGAGACTGAAGTCTGTCCCAGACCCACCGGCA24750.20542146675077189No Hit
CTATTACTGTCAGTTGTATGGTGAGTCATCGTGGACATTCGGCCAAGGGA24420.2026825138607616No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23900.1983665880946848No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23490.19496364662527804No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23180.19239069088011687No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAACGAGCAACCCTCTCCTGC22730.18865575512101193No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22630.18782576939676637No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21210.17603997211247968No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20980.1741310049467149No Hit
ATCTTACATCCAATAGGGCCACTGACATCCCAGACAGGTTCAGTGCCGGTGGGTCTGGGACAGACTTCAGTCTCA20920.1736330135121676No Hit
ACCCTCTCCTGCAGGGCCAGTCATAGTGTGGCTAGCGACTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCT20920.1736330135121676No Hit
CACTATGACTGGCCCTGCAGGAGAGGGTTGCTCGTTCCCCTGGAGACAAA19940.16549915341456126No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19860.16483516483516483No Hit
CAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGCGATGGTGAGACTGAAGTCTGTCCCAGACCCACCGGCA19630.16292619766940009No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19250.15977225191726704No Hit
GTCTTTGTCTCCAGGGGAACGAGCAACCCTCTCCTGCAGGGCCAGTCATA18840.1563693104478603No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATAC18750.1556223232960393No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18470.1532983632681518No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18470.1532983632681518No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18090.15014441751601873No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT17800.14773745891570664No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17530.1454964974602437No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17090.1418445602735633No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG16010.13288071445171143No Hit
GTTCAGTGCCGGTGGGTCTGGGACAGACTTCAGTCTCACCATCGCCAGAC15840.131469738720494No Hit
CTCCTGCAGGGCCAGTCATAGTGTGGCTAGCGACTATTTAGCCTGGTACC15790.13105474585837124No Hit
CATCTATCTTACATCCAATAGGGCCACTGACATCCCAGACAGGTTCAGTG15630.1297267686995784No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15620.1296437701271538No Hit
GTCCACGATGACTCACCATACAACTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGCGATGGTGAGA15210.1262408286577471No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAAGTCTCCTGCA15160.1258258357956243No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15120.1254938415059261No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14910.12375087148501047No Hit
GTAATAAGTGGCCGTGTCTTCAGATGTCAGGCCGCTCAGGTCCATGTAAA14840.12316988147803858No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14290.1186049599946881No Hit
ATCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATCAGGCGAACTGTGG14050.11661299425649879No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13820.11470402709073405No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCCTGATTTCCACCTTGGTCC13730.11395703993891307No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13700.11370804422163938No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13690.11362504564921484No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13670.11345904850436574No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT13560.11254606420769563No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA13460.11171607848345008No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13370.1109690913316291No Hit
CAATAGGGCCACTGACATCCCAGACAGGTTCAGTGCCGGTGGGTCTGGGA13360.11088609275920454No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13200.10955811560041168No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC13020.1080641412967697No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13020.1080641412967697No Hit
GACTTCAGTCTCACCATCGCCAGACTGGAGCCTGAAGATTTTGCAGTCTA12730.10565718269645763No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTC12690.10532518840675942No Hit
GCACTGAACCTGTCTGGGATGTCAGTGGCCCTATTGGATGTAAGATAGAT12480.10358221838584376No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12390.10283523123402279No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12360.10258623551674911No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12290.10200524550977724No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12250.10167325122007903No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT12220.10142425550280536No Hit
CTCTTCCTCCTGCTCCTCTGGCTCCCAGATACCACCGGAGAAATTGTGTT12070.10017927691643705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)