FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866800
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55560.6409783110290724No Hit
GAGTTACAGTACCCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT49270.56841255191509No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43800.5053068758652515No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA37750.43550992155053064No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37030.4272035071527457No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG36290.4186663590216889No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33140.3823257960313798No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTTCAAAGTCTTCAGGTTGCAGACTACTGATGGTGAGAGTGAAATCTGTC32250.3720581449007845No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31740.36617443470235345No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAGCTATTTAAATTGGTATCAGCAGAAA31600.36455929856945085No Hit
GTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT31410.36236732810336875No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29900.3449469312413475No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28930.333756345177665No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAG27560.31795108444854636No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27210.3139132441162898No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT26680.307798800184587No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG26510.30583756345177665No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25800.2976465159206276No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT25510.29430087678818645No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC25390.2929164743885556No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24900.2872634979233964No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG24510.2827641901245962No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24430.28184125519150904No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24130.27838024919243193No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23770.2742270419935394No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22850.2636132902630364No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22100.2549607752653438No Hit
ACAGTACCCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA22070.2546146746654361No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT22030.2541532071988925No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATGGGGTA21890.25253807106598986No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21270.2453853253345639No Hit
CTACTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT20500.23650207660359945No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20460.23604060913705585No Hit
GATTTCACTCTCACCATCAGTAGTCTGCAACCTGAAGACTTTGAAACTTACTACTGTCAACAGAGTTACAGTACC19450.22438855560682971No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19430.22415782187355793No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTTCAAAGTCTTCAGGTTGCAGACTACTGATGGTGAGAGTGAAAT19180.22127365020766038No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTA18860.2175819104753115No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17750.20477618827872635No Hit
GAGTATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATGCTGCATC17690.20408398707891093No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17640.20350715274573142No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17340.20004614674665436No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17210.19854637748038761No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16870.19462390401476698No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA16740.19312413474850024No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16570.1911628980156899No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC16490.1902399630826027No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16240.1873557914167051No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15620.1802030456852792No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15410.17778034148592525No Hit
GTATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT15340.17697277341947393No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA15240.1758191047531149No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15060.17374250115366865No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA14970.17270419935394554No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14950.17247346562067375No Hit
CACCATCAGTAGTCTGCAACCTGAAGACTTTGAAACTTACTACTGTCAACAGAGTTACAGTACCCCATTCACTTT14690.16947392708814027No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC14110.16278264882325796No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13990.16139824642362713No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13880.1601292108906322No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13800.159206275957545No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13680.15782187355791416No Hit
CCCCTAAGGTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCA13550.15632210429164745No Hit
GTAGTAAGTTTCAAAGTCTTCAGGTTGCAGACTACTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA13420.1548223350253807No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13320.15366866635902168No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12950.1494000922934933No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12900.1488232579603138No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12780.14743885556068298No Hit
CCATCAGTAGTCTGCAACCTGAAGACTTTGAAACTTACTACTGTCAACAG12710.14663128749423165No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12670.14616982002768805No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12610.14547761882787263No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGTAGTCTGCAACCTGAAGACTTTGAAACTTA12570.14501615136132903No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG12240.14120904476234425No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12230.14109367789570834No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA12220.14097831102907243No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATC12180.14051684356252886No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12040.1389017074296262No Hit
GTCTTCAGGTTGCAGACTACTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGAC11940.1377480387632672No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11880.1370558375634518No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11870.13694047069681586No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11840.13659437009690817No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11700.13497923396400552No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT11690.13486386709736964No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTG11680.13474850023073373No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11660.13451776649746194No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA11500.1326718966312875No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11490.1325565297646516No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11440.1319796954314721No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11310.13047992616520535No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11280.13013382556529765No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11210.12932625749884635No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11130.12840332256575912No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11080.1278264882325796No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11050.1274803876326719No Hit
CCTGAAGACTTTGAAACTTACTACTGTCAACAGAGTTACAGTACCCCATT11020.1271342870327642No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10900.12574988463313336No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10790.12448084910013843No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10450.12055837563451778No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10440.12044300876788187No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAG10410.12009690816797416No Hit
CTTTGAAACTTACTACTGTCAACAGAGTTACAGTACCCCATTCACTTTCG10280.11859713890170742No Hit
GCCCCTAAGGTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGAT10130.11686663590216889No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA9950.11479003230272265No Hit
GCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATGG9930.11455929856945087No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT9840.11352099676972774No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGTAGTCTGCAACCTGAAGACT9810.11317489616982004No Hit
ATGCAGCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTAATACT9760.1125980618366405No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT9710.11202122750346101No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9500.10959852330410706No Hit
GCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT9420.10867558837101983No Hit
CATCAGTAGTCTGCAACCTGAAGACTTTGAAACTTACTACTGTCAACAGAGTTACAGTACCCCATTCACTTTCGG9400.10844485463774804No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG9370.10809875403784033No Hit
GTTACAGTACCCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAA9370.10809875403784033No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG9340.10775265343793262No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9310.10740655283802492No Hit
GTTTCAAAGTCTTCAGGTTGCAGACTACTGATGGTGAGAGTGAAATCTGT9250.10671435163820951No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT9240.10659898477157359No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9160.10567604983848639No Hit
GCTAATACTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAG9160.10567604983848639No Hit
CTTTGGTCCCAGGGCCGAAAGTGAATGGGGTACTGTAACTCTGTTGACAG9130.10532994923857868No Hit
AGTCAGAGTATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATG9120.10521458237194277No Hit
CACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC9080.10475311490539918No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8990.10371481310567605No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGC8930.10302261190586062No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8890.10256114443931703No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG8880.10244577757268113No Hit
CTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGA8690.10025380710659898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA454.5838533E-1061.01681529
ACCGTAC454.6020432E-1060.985127
CGTATGC501.0986696E-955.2921641
GTACTAG501.1623342E-954.9437130
TATGTGG252.6543907E-454.8264246
TAGATCT552.6684575E-950.0269234
ATCTCGT507.010567E-848.24030337
TCGTATG652.1100277E-1047.82394440
TACTAGA652.2191671E-1047.57219731
ATGCCGT605.3969416E-946.20045544
GTGGTAT1250.043.8611371
GACCGTA651.1983502E-842.2131426
ACTAGAT759.0403773E-1041.24832532
GATCTCG602.9579314E-740.165336
CTAGATC602.9876537E-740.11414733
CACGAGA702.3195753E-839.1685121
TAACGGC451.0138563E-438.25266636
TTAACGG451.0173221E-438.23050335
GTATCAA20100.038.017081
ACGAGAC754.2555257E-836.56149722
Produced by FastQC (version 0.11.5)