FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262187
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTTATACTGTATCGTTTCTGCAAAGTGGTGTCCCGTCAAGGTTTAGTGGCAGTGGATCTGGGACAGATTTCA20550.7837917211761072No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15520.5919439178906658No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT12960.49430368401179314No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11860.45234889601696493No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10830.41306395816726227No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA10690.40772425787701144No Hit
GTATAAATCAGGAGCTTAGGGGCTTCCCCTGGTTTTTGCTGATACCAATT10300.39284937849702695No Hit
GAATTACATTATCCCGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT9720.3707277630088448No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9150.3489875546842521No Hit
ACATTATCCCGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA9130.3482247403570734No Hit
ATGTAATTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC9000.3432664472304119No Hit
GGCCAAAAGTGTGCGGGATAATGTAATTCTGTTGACAGTAGTAAGTTGCA8680.3310614179955528No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8400.32038201741505107No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7950.3032186950535305No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7720.29444633029097556No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7460.2845297440376525No Hit
GGATAATGTAATTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAAT7420.2830041153832951No Hit
GTGGTGTCCCGTCAAGGTTTAGTGGCAGTGGATCTGGGACAGATTTCACT7230.2757573792750975No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6850.26126390705870234No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6730.2566870210956302No Hit
GTAGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGAGCATTTACAACTATGTAAATTGGTATCAGCAAAAA6700.2555427996048622No Hit
CAATAATACAGGGCCGTGTCCGCGCCAGTCACAGAGCTCACCTTCAGGGAGAACTGGTTCTTAGACGTGTCTA6580.25096591364179No Hit
ATACTGTATCGTTTCTGCAAAGTGGTGTCCCGTCAAGGTTTAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA6410.2444819918607711No Hit
ACTTACTACTGTCAACAGAATTACATTATCCCGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA6390.24371917753359243No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6310.24066792022487765No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6290.23990510589769898No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAACAGAATTACATTATC6010.22922570531719727No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC5770.22007193339105297No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG5730.21854630473669556No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG5700.21740208324592752No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGAGCATTTACAA5520.2105367543013193No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5390.20557846117465775No Hit
ATTTACAACTATGTAAATTGGTATCAGCAAAAACCAGGGGAAGCCCCTAAGCTCCTGATTTATACTGTATCGTTT5390.20557846117465775No Hit
CCCCTAAGCTCCTGATTTATACTGTATCGTTTCTGCAAAGTGGTGTCCCG5380.2051970540110684No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT5130.1956618749213348No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT4770.1819312170321183No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT4650.17735433106904613No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4630.17659151674186746No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4560.17392166659674202No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC4540.17315885226956335No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4500.17163322361520594No Hit
GTAGTAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGA4430.16896337347008053No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA4350.16591211616136575No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4290.16362367317982965No Hit
ATGTAAATTGGTATCAGCAAAAACCAGGGGAAGCCCCTAAGCTCCTGATT4260.1624794516890616No Hit
GGACACCACTTTGCAGAAACGATACAGTATAAATCAGGAGCTTAGGGGCT4200.16019100870752553No Hit
GTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAACAGAATTACATTATCCCGCACACTTTTGGCCAGGGGA4180.15942819438034683No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4140.15790256572598946No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA4120.15713975139881076No Hit
CACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAAC4120.15713975139881076No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4110.15675834423522142No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4110.15675834423522142No Hit
GTTTAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTA4070.15523271558086404No Hit
GTATTATTGTGCGAGGGGTGGTCGAACTGGGACCCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC4040.154088494090096No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC3950.1506558296177919No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC3880.14798597947266645No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT3860.14722316514548778No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATT3800.14493472216395167No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3750.14302768634600493No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA3730.14226487201882626No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3650.13921361471011148No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT3630.13845080038293278No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG3580.13654376456498607No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA3570.1361623574013967No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGA3550.13539954307421803No Hit
GTCTCTGGTGTCTCCATCACCAATGGTGGTTACTACTGGAGCTGGATCCG3510.13387391441986063No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG3490.13311110009268196No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC3460.13196687860191392No Hit
ATACAGTATAAATCAGGAGCTTAGGGGCTTCCCCTGGTTTTTGCTGATACCAATTTACATAGTTGTAAATGCT3440.13120406427473522No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG3410.13005984278396718No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC3340.12738999263884174No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC3290.125482956820895No Hit
GGTTTAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGT3280.12510154965730566No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC3270.12472014249371632No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA3230.12319451383935892No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT3210.12243169951218025No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC3190.12166888518500155No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT3170.12090607085782284No Hit
AGTCAGAGCATTTACAACTATGTAAATTGGTATCAGCAAAAACCAGGGGAAGCCCCTAAGCTCCTGATTTATA3140.1197618493670548No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC3140.1197618493670548No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA3120.11899903503987612No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCC3080.11747340638551874No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG3070.11709199922192938No Hit
GTATCGTTTCTGCAAAGTGGTGTCCCGTCAAGGTTTAGTGGCAGTGGATC3070.11709199922192938No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG3060.11671059205834004No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT3050.1163291848947507No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG3000.11442214907680397No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT2980.11365933474962527No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC2980.11365933474962527No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTAAACCTTGACGGGACACCA2970.11327792758603593No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA2940.11213370609526789No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC2930.11175229893167853No Hit
GCTTAGGGGCTTCCCCTGGTTTTTGCTGATACCAATTTACATAGTTGTAA2920.11137089176808919No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC2920.11137089176808919No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA2900.11060807744091049No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA2850.10870104162296376No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT2850.10870104162296376No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA2850.10870104162296376No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGCCG2840.10831963445937443No Hit
GCAAAAACCAGGGGAAGCCCCTAAGCTCCTGATTTATACTGTATCGTTTC2770.10564978431424898No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC2730.10412415565989161No Hit
CCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAACAG2720.10374274849630225No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG2700.10297993416912357No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC2640.10069149118758748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACC200.00425468759.4200468
TTAACAC200.005056047356.88638367
GCGTATG252.56782E-455.18082440
ATCGCGT252.6479174E-454.83888237
AATCGCG252.675905E-454.72232436
GAATCGC252.6938375E-454.648435
TATAAGA252.8514967E-454.0228924
GGGAATA200.00800674650.6464618
CTTACAG200.00800674650.646463
CTTATAC2900.047.735751
GTGGTAT452.1527794E-645.0190771
TATACAC3050.044.281063
CCGTCTC904.838512E-1043.92963469
TTATACA3250.042.594972
TCTTATA804.3655746E-1142.2053832
ACCGTCT954.9112714E-1141.69827768
CTCTTAT858.367351E-1139.7227131
GATTCAC603.4331788E-739.3916931
TCACCGT959.276846E-1139.34696666
GGAATCG350.001426110338.96689634
Produced by FastQC (version 0.11.5)