FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262187
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30591.166724513419811No Hit
GATTTATACTGTATCGTTTCTGCAAAGTGGTGTCCCGTCAAGGTTTAGTGGCAGTGGATCTGGGACAGATTTCA17740.6766163082075007No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15530.5923253250542552No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11560.4409066811092846No Hit
ATGTAATTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC10000.38140716358934657No Hit
GAATTACATTATCCCGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT9800.3737790203175596No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9460.3608111767555218No Hit
ACATTATCCCGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA9390.3581413266103964No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8540.32572171770530195No Hit
GTATAAATCAGGAGCTTAGGGGCTTCCCCTGGTTTTTGCTGATACCAATT8340.318093574433515No Hit
GGCCAAAAGTGTGCGGGATAATGTAATTCTGTTGACAGTAGTAAGTTGCA8330.3177121672699257No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7920.30207447356276246No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7450.2841483368740632No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7390.2818598938925271No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7230.2757573792750975No Hit
GTGGTGTCCCGTCAAGGTTTAGTGGCAGTGGATCTGGGACAGATTTCACT7200.2746131577843295No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7200.2746131577843295No Hit
GGATAATGTAATTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAAT7080.27003627182125733No Hit
GTAGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGAGCATTTACAACTATGTAAATTGGTATCAGCAAAAA7060.26927345749407866No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6810.259738278404345No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGAGCATTTACAA6580.25096591364179No Hit
ACTTACTACTGTCAACAGAATTACATTATCCCGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA6490.2475332491694859No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6200.23647244142539484No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6140.23418399844385876No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6060.231132741135144No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAACAGAATTACATTATC6060.231132741135144No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG5930.22617444800848246No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5820.22197896920899968No Hit
CAATAATACAGGGCCGTGTCCGCGCCAGTCACAGAGCTCACCTTCAGGGAGAACTGGTTCTTAGACGTGTCTA5480.2090111256469619No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5300.20214579670235366No Hit
ATACTGTATCGTTTCTGCAAAGTGGTGTCCCGTCAAGGTTTAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA5300.20214579670235366No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC5220.1990945393936389No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG5040.19222921044903066No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC4910.18727091732236914No Hit
ATTTACAACTATGTAAATTGGTATCAGCAAAAACCAGGGGAAGCCCCTAAGCTCCTGATTTATACTGTATCGTTT4910.18727091732236914No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4840.18460106717724373No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4810.1834568456864757No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4670.17811714539622484No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT4660.1777357382326355No Hit
GTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAACAGAATTACATTATCCCGCACACTTTTGGCCAGGGGA4660.1777357382326355No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT4640.1769729239054568No Hit
CCCCTAAGCTCCTGATTTATACTGTATCGTTTCTGCAAAGTGGTGTCCCG4590.17506588808751006No Hit
GTAGTAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGA4530.17277744510597398No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4500.17163322361520594No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGA4430.16896337347008053No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4420.16858196630649117No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4220.16095382303470424No Hit
GTATTATTGTGCGAGGGGTGGTCGAACTGGGACCCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC4150.1582839728895788No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4110.15675834423522142No Hit
CACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAAC4110.15675834423522142No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4060.1548513084172747No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4050.15446990125368537No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3980.15180005110855993No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT3970.1514186439449706No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3940.15027442245420253No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA3900.14874879379984515No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG3840.14646035081830908No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC3810.14531612932754104No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTAAACCTTGACGGGACACCA3810.14531612932754104No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA3640.13883220754652212No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATT3640.13883220754652212No Hit
GGACACCACTTTGCAGAAACGATACAGTATAAATCAGGAGCTTAGGGGCT3610.13768798605575408No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA3470.13234828576550325No Hit
GTTTAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTA3460.13196687860191392No Hit
GTCTCTGGTGTCTCCATCACCAATGGTGGTTACTACTGGAGCTGGATCCG3450.13158547143832455No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC3430.13082265711114588No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC3420.13044124994755651No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG3360.12815280696602044No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC3360.12815280696602044No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT3310.1262457711480737No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA3300.12586436398448436No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC3290.125482956820895No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG3280.12510154965730566No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC3200.12205029234859088No Hit
ATGTAAATTGGTATCAGCAAAAACCAGGGGAAGCCCCTAAGCTCCTGATT3190.12166888518500155No Hit
GAGTCACCATCACCTGCCGGGCAAGTCAGAGCATTTACAACTATGTAAATTGGTATCAGCAAAAACCAGGGGAAG3050.1163291848947507No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT3050.1163291848947507No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC3050.1163291848947507No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC3040.11594777773116134No Hit
CCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAACAG3010.1148035562403933No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGCCG2970.11327792758603593No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA2930.11175229893167853No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTAAACCT2880.1098452631137318No Hit
ATACAGTATAAATCAGGAGCTTAGGGGCTTCCCCTGGTTTTTGCTGATACCAATTTACATAGTTGTAAATGCT2830.10793822729578506No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT2810.10717541296860639No Hit
GGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC2800.10679400580501702No Hit
GTTGTAAATGCTCTGACTTGCCCGGCAGGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA2750.10488696998707031No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC2740.10450556282348095No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG2730.10412415565989161No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT2720.10374274849630225No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT2700.10297993416912357No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTC2670.10183571267835553No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA2670.10183571267835553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTT150.002589629567.3560943
AAGTTCG350.00519141556.49463770
CCAATAC252.887748E-453.8848763
GGCACAT200.00675726352.86646750
TCCAATA200.00808811650.517072
GTATCAA6250.044.18561
GTGGTAT551.7693492E-742.862971
ACGGGAT504.4688622E-640.4831516
ACGGGAA350.001502514538.55537816
AGGTATC451.1518993E-437.420053
CAGGTAT451.1518993E-437.420052
TGACTTG3700.037.4086170
GTGGCCG1255.689635E-736.90983270
TATCAAC7750.036.0681042
ACGGGGC601.5604213E-533.7359616
GGGATAC601.570659E-533.70376218
TAAGTCA1002.4246729E-432.95520870
ATCAACG8500.032.8856243
TCAACGC8600.032.503234
CAACGCA8750.031.9460335
Produced by FastQC (version 0.11.5)