FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321308
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62670.47430273637940584No Hit
GAGTTACAGTACCCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT47830.3619897858788413No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47080.3563135922888532No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45740.34617212640807443No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44070.33353313534770096No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG43480.32906786305691027No Hit
GTAGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAGCTATTTAAATTGGTATCAGCAAAAA40270.3047737544917612No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA39160.2963729879785788No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36350.27510618266142334No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36210.2740466265246256No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32790.24816318375427984No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT32450.24558997599348525No Hit
CTGTAACTCTGTTGACAGTAGTAAATTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC32430.24543861083108556No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31480.23824876561710065No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAG31320.23703784431790315No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30760.23279961977071204No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29520.22341497970193172No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT29060.219933580966739No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC28970.21925243773594044No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG28630.21667922997514583No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28450.21531694351354871No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27750.2100191628295598No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG27610.208959606692762No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27600.20888392411156217No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27040.20464569956437106No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27020.20449433440197137No Hit
GTAATATACAGCCGTGTCCTCGCCTCTCAGGCTGTTCATTTGGAGATACA26840.20313204794037426No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25080.18981191364920214No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25010.18928213558080326No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAT24830.18791984911920612No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT24810.18776848395680643No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGGGAGGGGTA24500.18542232393961136No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTACTACTGTCAACAGAGTTACAGTACC23830.180351590999222No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23790.1800488606744226No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23180.17543222322123228No Hit
GGCCAAAAGTGGGAGGGGTACTGTAACTCTGTTGACAGTAGTAAATTGCA22810.17263196771683817No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22060.16695577412685006No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA21710.1643068837848556No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA21650.16385278829765657No Hit
GTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA21550.16309596248565816No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC21380.16180935860526086No Hit
GTATATTACTGTACGAGACCCCAAGGTCGAACTGGTATACCAGTGGCTGGCCTTGACTCCTGGGGCCAGGGAACC21260.16090116763086273No Hit
GTACTGTAACTCTGTTGACAGTAGTAAATTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT21250.1608254850496629No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20970.15870637277606733No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT20220.15303017918607925No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19870.1503812888440848No Hit
GTTACAGTACCCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA19520.14773239850209036No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT19470.14735398559609114No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19450.14720262043369145No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC19130.14478077783529653No Hit
GTAGTAAATTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA18770.14205620491210225No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18720.14167779200610306No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAATTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT18650.14114801393770415No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA18120.13713683713411257No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17850.13509340744171683No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17750.13433658162971843No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAGCTATTTAAATTGGTATCAGCAAAAACCAGGGAAAG17720.1341095338861189No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17370.13146064354412446No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17210.130249722244927No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17110.12949289643292858No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTACTACTGTCAAC16910.12797924480893175No Hit
ACAGTACCCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA16890.12782787964653206No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16830.12737378415933304No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT16680.1262385454413354No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT16550.12525467188573747No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16530.12510330672333778No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16490.12480057639853842No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16400.12411943316773984No Hit
ATTTACTACTGTCAACAGAGTTACAGTACCCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA16300.12336260735574144No Hit
GTCTGCAACCTGAAGATTTTGCAATTTACTACTGTCAACAGAGTTACAGTACCCCTCCCACTTTTGGCCAGGGGA15960.12078939959494683No Hit
GCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAATAGCTGCTA15650.11844323957775173No Hit
GTCCCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT15620.11821619183415222No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15600.11806482667175253No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15520.1174593660221538No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15430.11677822279135525No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTG15370.11632412730415617No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15140.11458342793655982No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15110.11435638019296031No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC15070.11405364986816094No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14840.11231295050056458No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG14780.11185885501336554No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTA14630.11072361629536791No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14540.11004247306456935No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14500.10973974273976998No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14450.10936132983377078No Hit
AGTCAGACCATTAGCAGCTATTTAAATTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATG14440.10928564725257095No Hit
CCATCCTCCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCG14410.10905859950897143No Hit
ATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAATAGCTGCTAATGGT14390.10890723434657174No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT14380.10883155176537188No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14010.10603129626097776No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13830.10466900979938061No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13640.10323104075658364No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13600.10292831043178426No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCA13400.10141465880778744No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG13310.10073351557698886No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13260.10035510267098965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG150.001424277878.32896468
GCACGCG301.811471E-459.23465369
CTAGCGG250.006542782353.31118869
TGCGTGT400.00660164953.18828670
GTGGTAT2850.047.8491551
GACAGGT1300.041.960031
GTATCAA27150.040.4338571
CCGTTAA350.00143374538.93633316
TCTGGAG11850.037.7030970
TATCAAC30800.035.2927322
TCAACGC31400.034.5934564
ATCAACG31550.034.3249553
GCCGTTA400.002762976634.0654215
CAACGCA32150.033.7864575
AACGCAG32500.033.632156
GGAAACG1102.7718474E-432.23532570
CGCAGAG35000.031.327148
CGATTAC450.00444172630.9183147
GCAGAGT35500.030.8859149
AGAGTAC35200.030.85893611
Produced by FastQC (version 0.11.5)