FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205258
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGCAATCCGGGGTCCCT143201.1881273553048393No Hit
ACTATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA94710.7858068562913499No Hit
GTTTAGGAGACTGTCCGGGTCTTTGCTGGTACCAAGCTAAGAAGTTCTGA94390.7831518230951382No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT93210.7733613881841067No Hit
GTATTACTATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT84960.7049113135942678No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC75950.6301555351634256No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC61570.5108449809086519No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT56710.4705216642411832No Hit
TTACTATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT55740.46247359486516576No Hit
GAGTATAGTAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT54210.4497792173957775No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52740.43758265865067897No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC48920.40588819987089897No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG48890.40563929050875414No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47990.39817200964440813No Hit
TAGTAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC47670.39551697644819617No Hit
GTATAGTAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT44480.36904961427345845No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT44070.36564785299081193No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT41550.34473946657064297No Hit
GTCTCCAGACTCCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAACATTCAGAA39310.32615423419715944No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC38410.3186869533328134No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37830.3138747056646793No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37760.3132939171530079No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA36430.3022589354312521No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC36350.3015951771321991No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG35850.29744668776311795No Hit
GACCCGGACAGTCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGCAA34840.28906673923757403No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32470.2694028996281294No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32460.26931992984074776No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG32190.267079745581444No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGTGAC31520.2615207698268752No Hit
GTTTATTACTGTCAGCAGTATTACTATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA31160.25853385748113683No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30510.2531408213013313No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29560.24525869150007715No Hit
CTCCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAACATTCAGAACTTCTTAG29190.24218880936695714No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA28880.23961674595812682No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC28860.23945080638336355No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28810.23903595744645545No Hit
CTGTGACAACAGGTGCCTACGGGGACATCGCGATGACCCAGTCTCCAGAC28700.23812328978525757No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28480.2362979544628619No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27930.23173461615687263No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27730.2300752204092402No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26360.21870835953795784No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26340.2185424199631946No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTACTATACT25170.20883495483954473No Hit
GTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGACTGTCCGGGTCTTTGCTGGTA25070.2080052569657285No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC24530.20352488844712088No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24470.20302706972283113No Hit
GGCCAAAAGTGTACGGAGTATAGTAATACTGCTGACAGTAATAAACTGCC23980.19896155014113162No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23880.19813185226731536No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC23360.193817423323471No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA23140.19199208800107528No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAACATTC21950.18211868330266218No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21880.1815378947909908No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21870.18145492500360919No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT21720.18021037819288485No Hit
CCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC21400.17755534499667291No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21010.1743195232887896No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20620.17108370158090633No Hit
GTCCGGGTCTTTGCTGGTACCAAGCTAAGAAGTTCTGAATGTTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCT20130.16701818199920682No Hit
GCTCATTTACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT20100.16676927263706193No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20080.16660333306229871No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19840.16461205816513974No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19840.16461205816513974No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC19550.16220593433107267No Hit
GTCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT19440.16129326666987484No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19350.16054653858344023No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19330.160380599008677No Hit
CTACGGGGACATCGCGATGACCCAGTCTCCAGACTCCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTG19140.15880417304842614No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA19100.15847229389889966No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG19060.15814041474937315No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT18950.1572277470881753No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18940.15714477730079368No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18520.1536600462307655No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC18480.15332816708123903No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18360.15233252963265956No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18150.1505901640976455No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG18110.150258284948119No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18050.14976046622382924No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18040.14967749643644762No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17700.14685652366547244No Hit
ATAGTAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT17590.14594385600427462No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17560.14569494664212973No Hit
CTTCTTAGCTTGGTACCAGCAAAGACCCGGACAGTCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGCAATC17420.14453336961878702No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA17310.14362070195758916No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16720.13872548450207342No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16410.1361534210932431No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16320.1354066930068085No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16320.1354066930068085No Hit
CTCCAGACTCCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC16240.1347429347077555No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC15730.13051147555129275No Hit
ACCATCAACTGCAAGTCCAGCCAGAACATTCAGAACTTCTTAGCTTGGTA15680.13009662661438462No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTACTATACTCCGTACACTTTTGGCCAGGGGACCAAG15560.12910098916580517No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15310.1270267444812646No Hit
ATTCAGAACTTCTTAGCTTGGTACCAGCAAAGACCCGGACAGTCTCCTAAACTGCTCATTTACTGGGCATCTACC15270.1266948653317381No Hit
ATTACTATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA15180.1259481372453035No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15060.12495249979672401No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14710.12204855723836723No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14690.12188261766360396No Hit
GTAAATGAGCAGTTTAGGAGACTGTCCGGGTCTTTGCTGGTACCAAGCTAAGAAGTTCTGAATGTTCTGGCTGGA14560.12080401042764287No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCT14350.11906164489262878No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG14210.11790006786928607No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14110.11707036999546985No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA14080.11682146063332498No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG14020.11632364190903524No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT13960.11582582318474549No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG13870.1150790950983109No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT13860.11499612531092927No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13780.1143323670118763No Hit
GCAAAGACCCGGACAGTCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATT13690.11358563892544168No Hit
GTACCAGCAAAGACCCGGACAGTCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGCAATCCGGGGTCCCTGA13580.11267297126424385No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA13430.1114284244535195No Hit
GGCCACCATCAACTGCAAGTCCAGCCAGAACATTCAGAACTTCTTAGCTTGGTACCAGCAAAGACCCGGACAGT13180.10935417976897892No Hit
CTACCCGGCAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGG12920.10719696529705672No Hit
GGATCTCTGTGACAACAGGTGCCTACGGGGACATCGCGATGACCCAGTCT12910.10711399550967511No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12900.10703102572229349No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG12820.1063672674232405No Hit
GCTCTGGATCTCTGTGACAACAGGTGCCTACGGGGACATCGCGATGACCCAGTCTCCAGACTCCCTGGCCGTGT12800.10620132784847724No Hit
CCATCAACTGCAAGTCCAGCCAGAACATTCAGAACTTCTTAGCTTGGTAC12500.10371223422702856No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT12430.1031314457153572No Hit
TTATTACTGTCAGCAGTATTACTATACTCCGTACACTTTTGGCCAGGGGA12410.10296550614059395No Hit
TACGGGGACATCGCGATGACCCAGTCTCCAGACTCCCTGGCCGTGTCTCT12410.10296550614059395No Hit
GATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGG12380.1027165967784491No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGC12340.10238471762892261No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC12210.10130611039296152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG150.00244925768.32464629
GTAGCGC150.002453298568.2962428
CGCATAA350.005639799455.34827470
TCGGCAG350.005639799455.34827470
GCTTAAT252.7261858E-454.5327071
TCGTATG700.054.22715840
CGAAAAT200.006297490653.82916363
CCGCATA250.00717145652.0883369
GTTAGTA200.007424507351.6343138
AGTTGAT25700.051.00512770
CGTATGC750.050.68867541
GGTATCA9800.045.5598531
ATGCCGT851.8189894E-1244.86498344
GTGGTAT1850.044.215711
GGTCTGG47500.044.04557470
CATGCGC903.6379788E-1241.7365928
ACGAGAC903.6379788E-1241.67595322
GCTATAC504.1779876E-640.8995321
AGACGAA350.001152867240.7015260
ACATGCG955.456968E-1239.5300727
Produced by FastQC (version 0.11.5)