FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908716
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37800.415971546665845No Hit
GTAATATACGGCCGTGTCCTCGGCTCTAAGGCTACTTAGTTCCAGATATA34930.3843885218264012No Hit
GACTTACACTGGTACCAGCAGAAACCAGGTCAGTCTCCAAAACTCCTCAT33460.3682118505671739No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32680.359628310715339No Hit
GTATATTACTGTGCGAGGGGGACGGTGGCAATACGAAGCGCTGAGTTCTT25660.2823764520488249No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24520.26983127841922006No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24360.26807055229576676No Hit
GAGTTATAGTTTTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT22970.25277424409826615No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22790.25079342720938114No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21660.23835829896249214No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21110.23230580291312136No Hit
ATATTACTGTCAGCAGAGTTATAGTTTTCCTCTCACTTTCGGCGGAGGGA20750.2283441691353514No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20530.2259231707156031No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19010.20919627254279666No Hit
GTGTAAGTCAGGACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTT18500.20358395802428922No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18110.19929218809837176No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18100.19918214271565593No Hit
TCCCAGTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG18020.1983017796539293No Hit
CAGTAATATGTTGCAGCATCTTCAGGTTCCAGGGTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA17380.19125887516011603No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17300.19037851209838938No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17060.1877374229132094No Hit
CTATATGAGTTGGCTCCGCCAGGCTCCAGGGAAGGGGCTAGACTGGCTTGCATTCATTAGTCCTAGCGGTCTCTC16980.18685705985148274No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG16590.1825652899255653No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16470.1812447453329753No Hit
CCATCAATACCCTGGAACCTGAAGATGCTGCAACATATTACTGTCAGCAG16400.1804744276539645No Hit
GATTTCACCCTCACCATCAATACCCTGGAACCTGAAGATGCTGCAACATATTACTGTCAGCAGAGTTATAGTTTT16400.1804744276539645No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16340.1798141553576695No Hit
ACATATTACTGTCAGCAGAGTTATAGTTTTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA16310.179484019209522No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15970.17574247619718372No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15520.1707904339749713No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14890.16385757486387387No Hit
GTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT14600.16066625876511473No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14320.15758498804907145No Hit
CTATAACTCTGCTGACAGTAATATGTTGCAGCATCTTCAGGTTCCAGGGTATTGATGGTGAGGGTGAAATCTGTC14270.15703476113549228No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13910.15307312735772233No Hit
CTCCAAAACTCCTCATCAAGTATGCTTCCCAGTCCTTCTCAGGGGTCCCC13760.15142244661698484No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC13590.14955167511081568No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13590.14955167511081568No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13300.14636035901205657No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13130.14448958750588742No Hit
CTCCAGGGACAACGCCAAGAATTCACTATATCTGGAACTAAGTAGCCTTAGAGCCGAGGACACGGCCGTATATTA13050.14360922444416077No Hit
GTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCTGCCGGGCCAGTCAGAGCATTGGTC12960.1426188159997183No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12920.14217863446885495No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC12750.14030786296268583No Hit
GTAATATGTTGCAGCATCTTCAGGTTCCAGGGTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA12700.13975763604910665No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12690.13964759066639082No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC12410.13656631995034751No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12130.13348504923430424No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11950.13150423234541925No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11930.13128414157998758No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATACCCTGGAAC11890.13084396004912427No Hit
GGACTGGGAAGCATACTTGATGAGGAGTTTTGGAGACTGACCTGGTTTCT11790.12974350622196593No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCACTGACAACTATATGAGTTGGC11490.12644214474049098No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG11280.12413119170345852No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11250.12380105555531101No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11230.12358096478987934No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11080.12193028404914187No Hit
GGGTTCCAGCCTCCAGGGGTGAAATTGTGCTGACTCAGTCTCCAGACTTT10960.12060973945655189No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10920.12016955792568855No Hit
GTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATACC10610.11675815106149776No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10580.11642801491335025No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10530.11587778799977111No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10520.11576774261705527No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10190.11213624498743283No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10130.11147597269113782No Hit
GTTTTGGAGACTGACCTGGTTTCTGCTGGTACCAGTGTAAGTCAGGACCA10120.11136592730842199No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10120.11136592730842199No Hit
CTCATATAGTTGTCAGTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCCTCCAGGCTTGACCAAC10020.11026547348126367No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9990.10993533733311617No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9960.10960520118496868No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA9940.10938511041953701No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9840.1082846565923787No Hit
GAGCATTGGTCCTGACTTACACTGGTACCAGCAGAAACCAGGTCAGTCTCCAAAACTCCTCATCAAGTATGCTTC9660.10630383970349372No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTAAGGCTACTTAGTTCCAGATA9570.10531343125905124No Hit
GTCCCTGGAGATGGTGAAGCGGCCCTGCGCAGAGTCGGCGTAATGTGTGG9540.10498329511090373No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT9490.10443306819732456No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9270.10201206977757628No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9220.1014618428639971No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATACCC9130.10047143441955463No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9120.10036138903683879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5550.049.1945721
TCGTATG605.5915734E-946.01658240
ATCTCGT651.1657903E-842.34486837
CGTATGC702.1558662E-839.4955541
AATGGTA4450.038.92719770
GTATCAA15550.038.628021
CACGAGA901.382432E-1037.9145621
CTTATAC1100.037.2313461
GACTTTA754.260619E-836.556933
ATGCCGT806.7868314E-834.6726144
CGTAGGA701.0461026E-634.18329628
CGAGACA1103.092282E-1134.13437323
TACACAT1201.8189894E-1234.1287355
ACGAGAC1004.292815E-1034.123122
CTTGCTA400.002740445334.12122316
TATCAAC17950.033.4632722
ATCAACG17850.033.2683453
TCAACGC17950.033.0830084
GCCGTCT851.1336306E-732.706246
CGTTGAA851.1767406E-732.5674942
Produced by FastQC (version 0.11.5)