FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908716
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38380.42235417886336324No Hit
GACTTACACTGGTACCAGCAGAAACCAGGTCAGTCTCCAAAACTCCTCAT32410.3566570853820116No Hit
GTAATATACGGCCGTGTCCTCGGCTCTAAGGCTACTTAGTTCCAGATATA32090.35313563313510493No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30450.33508819036970844No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25660.2823764520488249No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24940.27445318449328504No Hit
GTATATTACTGTGCGAGGGGGACGGTGGCAATACGAAGCGCTGAGTTCTT24190.26619978078959766No Hit
GAGTTATAGTTTTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT22430.2468317934316112No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21440.2359373005427438No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20590.22658344301189812No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20190.22218162770326483No Hit
ATATTACTGTCAGCAGAGTTATAGTTTTCCTCTCACTTTCGGCGGAGGGA18570.20435427570330003No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17960.1976415073576343No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17080.18795751367864105No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17030.1874072867650619No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16850.18542646987617695No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16840.1853164244934611No Hit
GTGTAAGTCAGGACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTT16670.18344565298729196No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16650.1832255622218603No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16560.1822351537774178No Hit
CAGTAATATGTTGCAGCATCTTCAGGTTCCAGGGTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA16150.1777232930860687No Hit
TCCCAGTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG16080.17695297540705787No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG15970.17574247619718372No Hit
CTATATGAGTTGGCTCCGCCAGGCTCCAGGGAAGGGGCTAGACTGGCTTGCATTCATTAGTCCTAGCGGTCTCTC15910.17508220390088872No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15760.17343152316015126No Hit
GATTTCACCCTCACCATCAATACCCTGGAACCTGAAGATGCTGCAACATATTACTGTCAGCAGAGTTATAGTTTT15590.1715607516539821No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15400.1694698893823813No Hit
CCATCAATACCCTGGAACCTGAAGATGCTGCAACATATTACTGTCAGCAG15230.16759911787621215No Hit
ACATATTACTGTCAGCAGAGTTATAGTTTTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA14770.16253703027128388No Hit
CTCCAAAACTCCTCATCAAGTATGCTTCCCAGTCCTTCTCAGGGGTCCCC14710.16187675797498888No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14640.16110644029597807No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14120.1553840803947548No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14100.15516398962932312No Hit
GTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT13690.15065212893797403No Hit
CTATAACTCTGCTGACAGTAATATGTTGCAGCATCTTCAGGTTCCAGGGTATTGATGGTGAGGGTGAAATCTGTC13330.14669049516020408No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13250.14581013209847743No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13180.1450398144194666No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12900.14195854370342328No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12860.14151836217255995No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12680.13953754528367499No Hit
GTAATATGTTGCAGCATCTTCAGGTTCCAGGGTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA12670.13942749990095915No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC12210.13436541229603088No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12040.13249464078986176No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11950.13150423234541925No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11890.13084396004912427No Hit
CTCCAGGGACAACGCCAAGAATTCACTATATCTGGAACTAAGTAGCCTTAGAGCCGAGGACACGGCCGTATATTA11800.12985355160468176No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11740.12919327930838678No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11520.12677228088863848No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11510.12666223550592265No Hit
GTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCTGCCGGGCCAGTCAGAGCATTGGTC11500.12655219012320681No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC11480.12633209935777515No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11330.12468141861703766No Hit
GGGTTCCAGCCTCCAGGGGTGAAATTGTGCTGACTCAGTCTCCAGACTTT11190.123140783259016No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATACCCTGGAAC11170.12292069249358435No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG11080.12193028404914187No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11050.12160014790099437No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10760.11840883180223524No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA10750.11829878641951941No Hit
GGACTGGGAAGCATACTTGATGAGGAGTTTTGGAGACTGACCTGGTTTCT10610.11675815106149776No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10510.11565769723433944No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCACTGACAACTATATGAGTTGGC10500.11554765185162362No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC10470.11521751570347612No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10390.11433715264174947No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10330.11367688034545446No Hit
GTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATACC10290.11323669881459113No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10210.1123563357528645No Hit
GGGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCT10140.11158601807385365No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9930.10927506503682119No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9800.10784447506151536No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9700.10674402123435704No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTAAGGCTACTTAGTTCCAGATA9580.10542347664176707No Hit
GTTTTGGAGACTGACCTGGTTTCTGCTGGTACCAGTGTAAGTCAGGACCA9570.10531343125905124No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9540.10498329511090373No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATACCC9370.1031125236047346No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9330.10267234207387127No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9280.10212211516029211No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9220.1014618428639971No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9220.1014618428639971No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9190.1011317067158496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGT150.00248226368.093016
GTGGTAT2200.054.1827851
GCGATTT200.00684792852.69967353
TGGTATC2800.042.5534442
GTATCAA18100.039.514511
ATGACGC350.001362821639.33894737
GGTATCA6900.038.5000651
TATCAAC20000.035.7448922
ATCAACG20350.035.2954643
AATGGTA3350.035.10918870
TCAACGC20650.034.6178444
TTAACGG400.002651053534.35276835
CAACGCA20950.034.1240045
GTACAAT400.002765575734.0577471
AACGCAG21050.033.9656336
TCGGTTT800.00338877532.6710570
ACGCAGA22150.032.2859577
CAGAGTA22800.031.08220710
AGAGTAC22700.031.0724611
AACGGCC450.00467390130.59695637
Produced by FastQC (version 0.11.5)