FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600832
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT72040.4500159916843241No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA64150.40072912085715423No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGCCATCTCCTG57850.3613745852156878No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC57410.3586260144724743No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC54360.33957342182065325No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTCAGTGCTGCTGCTTGTATATGAGCTGCAGTAATAAT53150.33201485227681604No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG51530.32189511454043895No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC51430.32127043937152683No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC51010.3186468036620957No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAGCACTGAAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT48860.30521628753048413No Hit
CTCCTGGACAGTCGATCGCCATCTCCTGCACTGGAACCAGCAGTGACGTT48560.30334226202374764No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC47140.2944718746251949No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC45770.2859138248110982No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC45190.28229070883140767No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC44210.27616889217606844No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT39850.24893305481149802No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACA37410.23369098069004118No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTCAGTGCTGCTGCTTGTATATGAGCTG37400.23362851317314995No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36780.2297555271258945No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTCAGTGCTGCTGC36630.2288185143725263No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT36400.22738176148402828No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGC35550.2220720225482749No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG35460.22150981489625393No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG35090.21919851677127894No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG35050.21894864670371408No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTCAGTGCTG34990.2185738416023668No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34230.21382631031863433No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGCCATCT32310.20183254707552076No Hit
CTTCAGTGCTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCC31240.19514852276816055No Hit
CTCCTGTACAGCCTCTGGATTCACCTTTAACAGCTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG30960.19339943229520648No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAT29610.18496631751489226No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG29270.1828424219405909No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG29060.18153060408587535No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC28500.17803242313996723No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC28340.17703294286970775No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG27620.17253528165354018No Hit
GCACTGAAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC27550.17209800903530165No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG26890.16797515292048135No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA25840.1614160636469036No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC24420.15254567624835086No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGC23740.14829788509974812No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA23010.1437377563666893No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG22330.1394899652180866No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGCGATCG22100.13805321232958861No Hit
GGACTGAACAGGGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21830.13636658937352578No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC21770.13599178427217848No Hit
ATATATTACTGTGCGAAAGGTCGTGGACTGGTAAGCCCTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC21290.13299334346140007No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTC20950.1308694478870987No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT20590.12862061727901491No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCA20400.12743373445808182No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC20230.12637178667093113No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC20200.12618438412025748No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC20200.12618438412025748No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT20150.1258720465358014No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19770.12349828089393516No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA19730.1232484108263703No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC19670.122873605725023No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG19610.1224988006236757No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGCACTGAAGT19250.1202499700155919No Hit
GTGCAGGAGATGGCGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG19170.11975022988046218No Hit
CCCCTGAGGGCCGATTACTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA19080.11918802222844121No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT18490.11550243873185942No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18470.11537750369807699No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC18440.11519010114740334No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG18290.1142530883940351No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT18130.11325360812377563No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA17680.11044256986367089No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17590.10988036221164993No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17570.1097554271778675No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT17550.10963049214408507No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC17440.10894334945828169No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17370.10850607684004317No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG17360.10844360932315196No Hit
GGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAATCGGCCCTCAGG17320.10819373925558709No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG17250.10775646663734857No Hit
GTCGATCGCCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA17180.10731919401911005No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC17150.1071317914684364No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG17050.10650711629952424No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17000.10619477871506816No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG16940.10581997361372086No Hit
CAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAATCGGCCCTCAGGGGTTTCTAATCG16570.10350867548874584No Hit
CTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGA16550.10338374045496342No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCA16500.10307140287050734No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGCCATCTCCTGCA16470.10288400031983368No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC16290.10175958501579178No Hit
GTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACATAGTTATAA16180.10107244232998841No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGT16150.10088503977931475No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16080.10044776716107624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1050.049.20802738
GGTATCA13550.047.7886921
GTATAGA452.0253392E-645.4411771
CGTATGC1300.045.20983541
GTGGTAT3550.044.1611481
TCGTATG1350.043.48441740
TGGTATC3700.041.4497222
CTAGCGC1650.041.3877328
TAGCGCT1700.040.19056729
ACTAGCG1700.040.1503427
GTATCAA36950.039.2921681
GATAGTA350.001430781138.953237
ACCGATG400.001502004338.56974468
GACTAGC1800.037.90079526
TCTGGAG12750.037.53117470
ACGAGAC1850.036.84880422
CACGAGA1800.035.97651721
TATACAC2100.035.7037853
ATGCCGT1850.035.634244
TATCAAC40500.035.595592
Produced by FastQC (version 0.11.5)