FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224073
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTATGATACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT89310.7296133482235129No Hit
CTCCTACGCTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT84270.688439333275058No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT83790.6845179985180623No Hit
ATGATACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA67640.5525814228399777No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60590.4949868185966033No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC59680.48755262145313233No Hit
GCGTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAACTAAATAATTCTTATTGTTGGAGGTGGATAAAACACT57340.4684361145127783No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC56970.4654134189709274No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT49580.4050412026080144No Hit
GAGTATCATAACACTGCTGACAATAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT49340.40308053522951653No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44960.36729835557193075No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44400.36272346502210245No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC41270.3371530946275263No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39640.32383689534856175No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC39330.3213043666513353No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38890.3177098097907559No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37500.3063542778902892No Hit
TCATAACACTGCTGACAATAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC35910.29336485650774097No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35470.28977029964716156No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35350.2887899659579126No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT35100.2867476041053107No Hit
GTTATGATACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA34950.28552218699374954No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT33600.27449343298969914No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG33500.27367648824865837No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32690.26705923584622815No Hit
ATCATAACACTGCTGACAATAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT31780.2596250387027571No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31530.2575826768501552No Hit
AACCAGGACAGCCTCCTACGCTGCTCATTTCCTGGGCATCTACCCGGGAA31430.2567657321091144No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31060.25374303656726355No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29920.24442986651939877No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC29730.24287767151142128No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29610.24189733782217235No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28900.2360970301607829No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC28300.23119536171453828No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC28010.22882622196552005No Hit
GGCTGAGGATGTGGCAGTTTATTATTGTCAGCAGTGTTATGATACTCCGC27580.2253133595790447No Hit
GTATCATAACACTGCTGACAATAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT27520.22482319273442025No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27460.22433302588979578No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTATTGTCAGCAGTGTTATGATACT26270.21461138347141062No Hit
TCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC26160.21371274425626574No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25920.2117520768777679No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATC24760.20227551788169498No Hit
GCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT24400.1993345168139482No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT24390.19925282233984412No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23980.19590334890157696No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23510.19206370861868532No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA23250.18993965229197932No Hit
GCCTCCTACGCTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT23230.18977626334377118No Hit
GTTTTATCCACCTCCAACAATAAGAATTATTTAGTTTGGTACCAGCAGAA23000.1878972904393774No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22910.1871620401724407No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22810.18634509543139993No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATCCACCTCCAA22070.1802997043476982No Hit
CACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTATTGTCAGCAGTGTTATGATACTCCGCTCACTTT22030.1799729264512819No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21980.17956445408076152No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCGTAGGAGGCTGTCCTGGTTTCTGCTGGTA21720.1774403977540555No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATG21540.17596989722018214No Hit
GTAATACACAGCCGTGTCCTCAGGTCTGAGGCTGTTCATTTGCAGATACA21440.17515295247914137No Hit
GTCCTGGTTTCTGCTGGTACCAAACTAAATAATTCTTATTGTTGGAGGTG21160.1728655072042272No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20770.1696794227141682No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT20760.1695977282400641No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20500.1674736719133581No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20460.1671468940169418No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC20030.16363403163046647No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19680.1607747250368238No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19450.15889575213243No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19400.15848727976190963No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19360.1581605018654933No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGAT19300.15767033502086886No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19180.15669000133161992No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19140.15636322343520362No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18830.15383069473797722No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTT18690.15268697210052015No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18650.15236019420410385No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG18570.15170663841127122No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18340.14982766550687743No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG18320.1496642765586693No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18250.14909241523994077No Hit
GTTTATTATTGTCAGCAGTGTTATGATACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA17570.1435371910008635No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17410.14223007941519827No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT17380.14198499599288605No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17330.14157652362236567No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17100.13969755071797188No Hit
GGATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA16770.13700163307253735No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT16730.13667485517612105No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16240.13267182594502125No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16040.1310379364629397No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16030.13095624198883563No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG15850.12948574145496225No Hit
GGATGTGGCAGTTTATTATTGTCAGCAGTGTTATGATACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGAT15800.12907726908444184No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA15770.12883218566212962No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15620.12760676855056846No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15600.1274433796023603No Hit
ATAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA15470.12638135143900733No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG15450.12621796249079917No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15390.1257277956461747No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG15390.1257277956461747No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC15370.12556440669796656No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15260.1246657674828217No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC15250.12458407300871763No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15240.12450237853461354No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14990.1224600166820116No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14770.12066273825172191No Hit
GTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAACTAAATAATTCTTATTGTTGGAGGTGGATAAAACACTCTGG14590.11919223771784852No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14540.11878376534732812No Hit
CTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA14140.11551598638316506No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTATCATAACACTGCTGACAATAATAAACTGCCACATCCTCAGC14100.11518920848674874No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG13900.1135553190046672No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13790.11265667978952235No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG13690.11183973504848158No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG13600.1111044847815449No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT13580.11094109583333675No Hit
TTATTATTGTCAGCAGTGTTATGATACTCCGCTCACTTTCGGCGGAGGGA13560.11077770688512859No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG13510.1103692345146082No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA13390.10938890082535926No Hit
GCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC13340.10898042845483889No Hit
GTGTATTACTGTGCGAAGGGGTGCCGGGCTGGTGGTGGTGGGTTCTGCTA13330.10889873398073481No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13010.10628451080940435No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12910.10546756606836358No Hit
CAATAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA12660.10342520421576164No Hit
GCAGAAACCAGGACAGCCTCCTACGCTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT12640.10326181526755349No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA12590.10285334289703309No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12520.10228148157830456No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT12450.10170962025957601No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT12350.10089267551853524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC209.375036E-567.6710716
GTCGGTT250.006401606453.60565669
TGGCGTA306.982769E-445.066134
GGTATCA7950.044.65741
ATTAGAG651.36442395E-841.5995063
GTGGTAT2500.040.5744321
TGGTATC2550.039.7658582
TATAAGG350.001490806138.6281134
GTATCAA18650.036.2595521
ATACGTA952.7102942E-1035.60761611
GCTTGAT400.002865891433.812031
TATTAGA906.339178E-933.800982
TACGTAC1057.9489837E-1032.2177312
AATACGT1057.9489837E-1032.2164110
CGACCGG3100.031.64483311
GGTCTGG39500.031.41339770
TATCAAC21550.031.212972
ATGGCAA2001.5417754E-830.84934670
CGTACCA1101.3078534E-930.75077614
TAGACTA450.005108519530.0465435
Produced by FastQC (version 0.11.5)