FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013149
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCCCGTCTGTCAGGCTGTTCATTTGAAGATACA67890.6700889997423873No Hit
GTATAATAGTTATTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT51680.5100927899055321No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC46300.45699102501211575No Hit
GTGTATTACTGTGCAAGGGAAATCTCGGATAGCATGGAGCTCGACTGGTTCTTCGATCTCTGGGGCCGTGGCACC34590.34141078952848986No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31050.3064702230372828No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30190.2979818368275545No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29850.2946259632097549No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24260.23945145284652108No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23030.22731108652330506No Hit
GGTCTGGAGTGGGTCTCAGCTATTGGTAGAGGTGGTGACACATACTATCC22580.2228694890879821No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22390.22099414794862354No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21070.20796546213834294No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20200.19937837376338524No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20120.19858875644155005No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20040.19779913911971486No Hit
CAGTAATACACAGCCGTGTCCCCGTCTGTCAGGCTGTTCATTTGAAGATA19900.19641730880650327No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19690.19434456333668593No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19500.19246922219732734No Hit
CTCCAGACCTTTGCCTGCAGTTTGGCGGACCCAGTGCATGTCGGAGCTAC19440.19187700920595097No Hit
CTCTTGTGCAGCCTCTGGATTCACCTTCAGTAGCTCCGACATGCACTGGGTCCGCCAAACTGCAGGCAAAGGT19420.19167960487549215No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18150.1791444298913585No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17290.17065604368163026No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17030.16808978738566588No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16400.16187155097621375No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG16320.1610819336543786No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16280.160687124993461No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16090.1588117838541024No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15570.15367927126217368No Hit
GGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC15550.1534818669317149No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15510.1530870582707973No Hit
GATCAGGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGG15440.1523961431141915No Hit
CCCTGAGGAGACAGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGAACC15310.15111301496620932No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15080.14884286516593315No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15050.14854675867024494No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14620.14430256556538082No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATATTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT14570.14380905473923383No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAGCGGGTTGGCCTGGTATCAGCAGAAA14520.14331554391308682No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14460.14272333092171044No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14150.13966356379959907No Hit
GCCTGGGGGGTCCCTGAGACTCTCTTGTGCAGCCTCTGGATTCACCTTCAGTAGCTCCGACATGCACTGGGTCCG13670.13492585986858793No Hit
GTATGTGTCACCACCTCTACCAATAGCTGAGACCCACTCCAGACCTTTGC13600.13423494471198213No Hit
GACACATACTATCCGGGCTCCGTGAAGGGCCGATTCACCATCTCCAGAGAAAATGCCAAGAACTCCTTGTATCTT13550.13374143388583518No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACTTTGCAACTTA13530.13354402955537636No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA13410.13235960357262358No Hit
GACTATTAGTAGCGGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGCTCCTGATCTATAAGGCGT13310.13137258192032958No Hit
GTATTACTGTGCAAGGGAAATCTCGGATAGCATGGAGCTCGACTGGTTCTTCGATCTCTGGGGCCGTGGCACC13230.1305829645984944No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13050.12880632562436523No Hit
CAGTATAATAGTTATTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT12750.12584526066748328No Hit
GGGTGCCACGGCCCCAGAGATCGAAGAACCAGTCGAGCTCCATGCTATCCGAGATTTCCCTTGCACAGTAATACA12700.12535174984133626No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA12650.12485823901518928No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG12550.12387121736289529No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT12370.12209457838876611No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12240.12081145024078392No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12160.12002183291894873No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC12160.12002183291894873No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12080.11923221559711356No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT12030.11873870477096657No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12030.11873870477096657No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12000.11844259827527837No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT11950.11794908744913137No Hit
GCCCCAGAGATCGAAGAACCAGTCGAGCTCCATGCTATCCGAGATTTCCCTTGCACAGTAATACACAGCCGTGTC11920.11765298095344319No Hit
GCCTTGGGATTCCCAAGTGTTTGTATTCAGTGATCAGGACTGAACACACA11790.116369852805461No Hit
GGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATATTAGAAGGTGTCCAGTGTGAGGTGCAA11670.11518542682270821No Hit
GTGTTTGTATTCAGTGATCAGGACTGAACACACAGGACTCACCATGGAGT11590.11439580950087302No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC11440.11291527702243204No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT11420.11271787269197325No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGCCAACCCGCTACTAATAGTCTGA11340.11192825537013806No Hit
GACATGCACTGGGTCCGCCAAACTGCAGGCAAAGGTCTGGAGTGGGTCTC11000.10857238175233849No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC10990.1084736795871091No Hit
GGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCTTGTGCAGCCTCTGGATTCACCTTCAGTAGCTCCGACATG10900.10758536010004452No Hit
GTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCTTGTGCAGCCTCTGGATTCACCTTCAGTAG10840.10699314710866813No Hit
GTCCTGTGCGAGGCAGCCAACGGCCACGCTGCTCGTATCCGACGGGGAAT10830.10689444494343874No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG10700.10561131679545654No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10580.10442689081270376No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10550.10413078431701557No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC10400.10265025183857458No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10390.10255154967334518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCG205.1931746E-4100.9654770
TCGCATC150.002511649267.89175412
TATACTA200.00784677150.9162985
CTTATAC1950.046.999661
ATGCCGT1800.046.17514444
CGTATGC1800.045.95529641
TCGTATG1850.044.66177440
TATACAC2000.044.127463
GCCGTCT1900.043.89074746
TGCCGTC1950.042.6941645
AATGGTA3000.042.62986870
GTATGCC1950.042.4862842
GACATAA405.5045944E-542.430252
ATCTCGT1800.041.90725737
GTGGTAT1250.040.733041
ACGAGAC2250.039.23409322
TTATACA2250.039.2244072
GTATCAA15350.038.0351941
CACGAGA2350.037.5645621
TCTGGAG33000.036.71471870
Produced by FastQC (version 0.11.5)