FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013149
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCCCGTCTGTCAGGCTGTTCATTTGAAGATACA59420.5864882657930867No Hit
GTATAATAGTTATTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT48040.47416520176203103No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC41330.40793604889310453No Hit
GTGTATTACTGTGCAAGGGAAATCTCGGATAGCATGGAGCTCGACTGGTTCTTCGATCTCTGGGGCCGTGGCACC34900.3444705566506012No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30320.2992649649755367No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29590.2920597069137906No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26740.26392958982341197No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24550.24231381563817367No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23660.23352932293275716No Hit
GGTCTGGAGTGGGTCTCAGCTATTGGTAGAGGTGGTGACACATACTATCC23160.22859421467128724No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23030.22731108652330506No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21720.21438110287825382No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20380.20115501273751443No Hit
CTCCAGACCTTTGCCTGCAGTTTGGCGGACCCAGTGCATGTCGGAGCTAC19900.19641730880650327No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19100.1885211355881514No Hit
CTCTTGTGCAGCCTCTGGATTCACCTTCAGTAGCTCCGACATGCACTGGGTCCGCCAAACTGCAGGCAAAGGT18350.1811184731959465No Hit
CAGTAATACACAGCCGTGTCCCCGTCTGTCAGGCTGTTCATTTGAAGATA17440.17213657616007122No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17420.1719391718296124No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17240.17016253285548325No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17170.16947161769887745No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16980.1675962765595189No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16450.16236506180236077No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16320.1610819336543786No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15870.15664033621905565No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15760.15555461240153223No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15650.15446888858400887No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15410.1521000366185033No Hit
CCCTGAGGAGACAGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGAACC15270.15071820630529173No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15260.15061950414006234No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15250.15052080197483292No Hit
GGGTGCCACGGCCCCAGAGATCGAAGAACCAGTCGAGCTCCATGCTATCCGAGATTTCCCTTGCACAGTAATACA14790.1459805023742806No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14740.14548699154813358No Hit
GATCAGGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGG14720.1452895872176748No Hit
GGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC14630.1444012677306102No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATATTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT14290.14104539411281064No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG14040.13857783998207568No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13820.13640639234702892No Hit
GCCTGGGGGGTCCCTGAGACTCTCTTGTGCAGCCTCTGGATTCACCTTCAGTAGCTCCGACATGCACTGGGTCCG13810.13630769018179953No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13770.13591288152088193No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13380.13206349707693538No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13370.13196479491170598No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13270.13097777325941198No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG13250.1307803689289532No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12940.12772060180684183No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAGCGGGTTGGCCTGGTATCAGCAGAAA12920.12752319747638302No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACTTTGCAACTTA12760.12594396283271267No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA12690.12525304767610687No Hit
GCCTTGGGATTCCCAAGTGTTTGTATTCAGTGATCAGGACTGAACACACA12680.12515434551087748No Hit
GTATGTGTCACCACCTCTACCAATAGCTGAGACCCACTCCAGACCTTTGC12610.1244634303542717No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA12580.12416732385858349No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT12410.1224893870496837No Hit
GTCCTGTGCGAGGCAGCCAACGGCCACGCTGCTCGTATCCGACGGGGAAT12370.12209457838876611No Hit
GACACATACTATCCGGGCTCCGTGAAGGGCCGATTCACCATCTCCAGAGAAAATGCCAAGAACTCCTTGTATCTT12300.12140366323216033No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12250.12091015240601334No Hit
GTGTTTGTATTCAGTGATCAGGACTGAACACACAGGACTCACCATGGAGT12090.11933091776234295No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11980.11824519394481955No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11790.116369852805461No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC11710.11558023548362581No Hit
GTATTACTGTGCAAGGGAAATCTCGGATAGCATGGAGCTCGACTGGTTCTTCGATCTCTGGGGCCGTGGCACC11700.11548153331839642No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11650.1149880224922494No Hit
GACTATTAGTAGCGGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGCTCCTGATCTATAAGGCGT11580.11429710733564362No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11580.11429710733564362No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT11560.11409970300518484No Hit
GCCCCAGAGATCGAAGAACCAGTCGAGCTCCATGCTATCCGAGATTTCCCTTGCACAGTAATACACAGCCGTGTC11540.11390229867472602No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA11540.11390229867472602No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC11250.11103993588307347No Hit
GTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCTTGTGCAGCCTCTGGATTCACCTTCAGTAG11230.11084253155261468No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC11010.10867108391756791No Hit
GGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATATTAGAAGGTGTCCAGTGTGAGGTGCAA10930.10788146659573271No Hit
GGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCTTGTGCAGCCTCTGGATTCACCTTCAGTAGCTCCGACATG10870.10728925360435633No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC10820.10679574277820932No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT10520.10383467782132737No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10520.10383467782132737No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG10480.10343986916040977No Hit
CAGTATAATAGTTATTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT10460.10324246482995096No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10280.10146582585582178No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT10230.10097231502967481No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10190.10057750636875722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC150.002528506367.77731
AATCATA150.002529001367.773956
CGAAACC301.9243167E-458.5132769
AAATCAT252.806093E-454.213815
GTGGTAT2200.040.0502241
CCGAAAC400.001327992239.5465268
CATAATG350.001463696238.77199616
CCGTTAA350.001463696238.77199616
GTGTTTA350.001471534438.7298851
GTATCAA13700.038.341171
ATATACT558.3695795E-636.962144
AATGGTA2251.8189894E-1236.40308870
TGCACAA9750.036.40308870
CGAACTA502.0210753E-434.0196529
TAATGAA502.0530596E-433.92885218
CCCTATA400.002836097533.8836332
TCGGTTG553.4584073E-431.04387932
TACACCG553.6197965E-430.80335
ACTAGAA450.004972168730.21273828
TATCAAC17400.030.1836952
Produced by FastQC (version 0.11.5)