FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764476
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACACAGACCGGGGAAAGCTCCTAAGCTCCTGATCTTTGATGCCT25960.33957900575034405No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25220.32989917276670555No Hit
GTGTTGATACCAGGCTAAAGCACTGGCAATGCCCTGACTTGCCCGGCAAG22530.2947116717856414No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22360.2924879263704812No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20150.2635792359733988No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20070.26253276754273513No Hit
GGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT17270.2259063724695085No Hit
ACTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT17030.22276696717751765No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15460.20223002422574418No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15360.20092193868741465No Hit
AGTCAGGGCATTGCCAGTGCTTTAGCCTGGTATCAACACAGACCGGGGAA15130.19791334194925675No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14970.1958204050879295No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14940.19542797942643064No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14180.18548652933512627No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14060.18391682668913084No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13810.18064661284330705No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTGCCAGTGCTTTAGCCTGGTATCAACACAGA13750.17986176152030933No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13600.17789963321281504No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13460.17606831345915372No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13440.17580669635148782No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAATTTGATTCTTAT13340.1744986108131583No Hit
GCTTTAGCCTGGTATCAACACAGACCGGGGAAAGCTCCTAAGCTCCTGAT13310.17410618515165943No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12810.16756575746001182No Hit
ACTTATTACTGTCAACAATTTGATTCTTATCCTAATGGGCTCACTTTCGGCGGAGGGACCCAGGTGCAGATCAAA11690.15291519943072118No Hit
GACCCACTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGG11440.14964498558489736No Hit
GTGGAGTCCCATCGAGGTTCAGCGGCAGCGGATCTGGGACAGACTTCACT11050.1445434519854122No Hit
GCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG10830.14166566380108728No Hit
GATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGAT10700.13996515260125889No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10660.1394419183859271No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG10600.13865706706292938No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10530.13774140718609873No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10480.13708736441693395No Hit
GGACTGAACAGAGAGAGCTCGCCATGGAGTTTGGGCTGAGCTGGCTTTTT10390.13591008743243738No Hit
GCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGTCATCCAGTTGACCCAGTCTCCGTCGTCC10360.13551766177093852No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTGGGTCCCTCCGCCGAAAGTG10300.1347328104479408No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10230.13381715057111015No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10040.13133178804828405No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9870.12910804263312387No Hit
ACACAGACCGGGGAAAGCTCCTAAGCTCCTGATCTTTGATGCCTCCACTTTGGAAAGTGGAGTCCCATCGAGGTT9800.1281923827562932No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9760.1276691485409614No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9640.12609944589496597No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9410.12309084915680807No Hit
CTCCTGTGGTGCCTCTGGATTCACTTTTAGCGCCTATGCCATGACCTGGGTCCGCCAGGCTCCCGGGAAGGGG9360.1224368063876433No Hit
GTAATATAAGGCCGTGTCGTCGGCTCTCAGGGTGCTCATCTGCAGATAGA9240.12086710374164787No Hit
GCCTTATATTACTGTGCGAAAGGCGAGAGATCATCTATAGAGACTGCCATGGGTTACTACTTTGACTTTTGGGGC9230.12073629518781492No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8870.11602718724982863No Hit
ATCCTAATGGGCTCACTTTCGGCGGAGGGACCCAGGTGCAGATCAAACGA8810.11524233592683092No Hit
GGACTCCACTTTCCAAAGTGGAGGCATCAAAGATCAGGAGCTTAGGAGCTTTCCCCGGTCTGTGTTGATACCAGG8810.11524233592683092No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8790.11498071881916502No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8760.11458829315766617No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8640.11301859051167074No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8630.1128877819578378No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTGCACCTGGGTCC8590.11236454774250597No Hit
CCCAGGTGCAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT8590.11236454774250597No Hit
GGCTAAAGCACTGGCAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAGACAGGGA8470.11079484509651054No Hit
GGGCATTGCCAGTGCTTTAGCCTGGTATCAACACAGACCGGGGAAAGCTCCTAAGCTCCTGATCTTTGATGCCTC8450.11053322798884466No Hit
GAACAGAGAGAGCTCGCCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT8400.10987918521967988No Hit
CTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTC8360.10935595100434808No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGTCATCCAGTTGACCCAGTC8250.10791705691218559No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT8140.10647816282002313No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8100.10595492860469133No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8020.1049084601740277No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8020.1049084601740277No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7910.10346956608186522No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7880.10307714042036636No Hit
GCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAGACAGGGACGACGGAGACTGGGTCAA7780.10176905488203684No Hit
GTGCCAGATGTGTCATCCAGTTGACCCAGTCTCCGTCGTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCA7770.1016382463282039No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7720.10098420355903913No Hit
CAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGTCAT7650.10006854368220847No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)