FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764476
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26130.34180275116550424No Hit
GTGTTGATACCAGGCTAAAGCACTGGCAATGCCCTGACTTGCCCGGCAAG22250.29104903227831874No Hit
GTATCAACACAGACCGGGGAAAGCTCCTAAGCTCCTGATCTTTGATGCCT19090.24971352926710585No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18760.24539684699061842No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17030.22276696717751765No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17000.2223745415160188No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16870.22067403031619046No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15920.20824721770205998No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15680.20510781241006912No Hit
GGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT14810.19372746822660228No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14750.19294261690360456No Hit
GAGTGAAGTCTGTCCCAGATCCGCTGCCGCTGAACCTCGATGGGACTCCA14320.18731784908878762No Hit
ACTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT13580.17763801610514915No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAATTTGATTCTTAT13480.17632993056681964No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12830.16782737456767774No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12520.1637723093988562No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12420.1624642238605267No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11940.15618541327654498No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCGCTGCCG11870.15526975339971433No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11850.1550081362920484No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11380.14886013426189965No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11280.14755204872357014No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11040.14441264343157928No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10930.1429737493394168No Hit
ACTTATTACTGTCAACAATTTGATTCTTATCCTAATGGGCTCACTTTCGGCGGAGGGACCCAGGTGCAGATCAAA10890.142450515124085No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTGCCAGTGCTTTAGCCTGGTATCAACACAGA10860.14205808946258613No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10840.14179647235492024No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTGGGTCCCTCCGCCGAAAGTG10600.13865706706292938No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10510.1374797900784328No Hit
GTCCCAGATCCGCTGCCGCTGAACCTCGATGGGACTCCACTTTCCAAAGT10360.13551766177093852No Hit
GCTTTAGCCTGGTATCAACACAGACCGGGGAAAGCTCCTAAGCTCCTGAT10270.13434038478644195No Hit
GATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGAT9920.12976208540228862No Hit
AGTCAGGGCATTGCCAGTGCTTTAGCCTGGTATCAACACAGACCGGGGAA9710.12701510577179662No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9670.12649187155646482No Hit
CTCCTGTGGTGCCTCTGGATTCACTTTTAGCGCCTATGCCATGACCTGGGTCCGCCAGGCTCCCGGGAAGGGG9550.1249221689104694No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9430.12335246626447396No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9210.12047467808014901No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9160.11982063531098426No Hit
GACCCACTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGG9150.1196898267571513No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9120.11929740109565244No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTGCACCTGGGTCC8850.11576557014216274No Hit
GCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG8750.1144574846038332No Hit
GTGGAGTCCCATCGAGGTTCAGCGGCAGCGGATCTGGGACAGACTTCACT8610.11262616485017188No Hit
GGACTGAACAGAGAGAGCTCGCCATGGAGTTTGGGCTGAGCTGGCTTTTT8570.11210293063484009No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8520.1114488878656753No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG8510.11131807931184237No Hit
GGACTCCACTTTCCAAAGTGGAGGCATCAAAGATCAGGAGCTTAGGAGCTTTCCCCGGTCTGTGTTGATACCAGG8490.11105646220417645No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8360.10935595100434808No Hit
GCCTTATATTACTGTGCGAAAGGCGAGAGATCATCTATAGAGACTGCCATGGGTTACTACTTTGACTTTTGGGGC8340.10909433389668216No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8330.10896352534284923No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8250.10791705691218559No Hit
GCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGTCATCCAGTTGACCCAGTCTCCGTCGTCC8240.10778624835835265No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8150.10660897137385608No Hit
CATTAGGATAAGAATCAAATTGTTGACAGTAATAAGTTGCAAAATCTTCA8050.10530088583552656No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7990.10451603451252885No Hit
ATCCTAATGGGCTCACTTTCGGCGGAGGGACCCAGGTGCAGATCAAACGA7980.1043852259586959No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7920.10360037463569818No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7860.10281552331270047No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7850.10268471475886752No Hit
GGCTAAAGCACTGGCAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAGACAGGGA7790.1018998634358698No Hit
CCCAGGTGCAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT7660.10019935223604141No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG200.0079162750.801642
CTAACGC200.00793061850.7783783
GTGGTAT2150.048.8549651
CGCACCT306.397604E-445.8715540
AAACGTG400.00221741535.62585457
ATACCGC450.00449493930.84144845
TATACTA450.00507388130.0869525
GCACATA1000.006604572828.50264570
AATGGTA2502.930392E-928.50264470
TGTTAGA10050.028.36084270
ATCAACG16600.027.9383033
CAACGCA16950.027.1581335
ATCTAAC500.00844467627.1066271
GTCTAAG500.00844467627.1066271
CATTAAT500.00844467627.1066271
GCGTTAG500.00846087227.0959817
TACTAAG500.008490629527.0764877
GACCTAT500.008490629527.0764876
TCAACGC16950.026.958444
AACGCAG17600.026.1534256
Produced by FastQC (version 0.11.5)