FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355487
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT82450.6082684673478977No Hit
GTATAATGATTGGCCTCCGCTCTCATTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT76510.5644465789786254No Hit
TCATTATACTGCTGACAATAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGAGAGACTGAACTCTGTC71520.5276332417795228No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC65410.48255719162190414No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA63970.47193370353238356No Hit
GTCTGGGACAGAGTTCAGTCTCTCCATCAGCAGCCTGCAGTCTGAAGATT51940.3831833134511803No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50100.369608856447904No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46510.3431239104469464No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT46160.3405418126474101No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44450.32792642054110444No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTGAGACTATTAGCAG44380.32741000098119716No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42420.3129502533037941No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG40620.2996708931918934No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36670.27053007516855565No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35880.2647019115638881No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34860.257176940833811No Hit
GAGTTCAGTCTCTCCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTATTGTCAGCAGTATAATGATTGG34770.256512972828216No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34650.25562768215408926No Hit
CACCAGGGCCGCTGGTGTCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGAGTTCAGTCTCTCCATCAGCAG33870.2498732927722656No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTGAGACTATTAGCAGCCACCTAGCCTGGTA33620.24802893720116828No Hit
CTCCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTATTGTCAGC32800.24197945092796908No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAATGAGAGCGGAGGCCAATCATTATACTG32550.24013509535687175No Hit
CATTTATGGTGCATCCACCAGGGCCGCTGGTGTCCCAGCCAGGTTCAGCG31610.2332003184095458No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31500.23238880195826297No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31360.23135596283844848No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30860.22766725169625382No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC30790.22715083213634657No Hit
GTCCCAGACCCACTGCCGCTGAACCTGGCTGGGACACCAGCGGCCCTGGT30090.22198663653727407No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29850.22021605518902063No Hit
CTTCTGCAGGGCCAGTGAGACTATTAGCAGCCACCTAGCCTGGTACCAGCAGAAAGCTGGCCAGGCTCCCAGGCT28870.21298618135031913No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28540.21055163199647067No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28080.20715801774565157No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT26520.19564923898200423No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGCCAGCTTTCTGCTGGTACCAGGC25720.18974730115449281No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC25610.18893578470320999No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24500.18074684596753787No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24090.17772210283093826No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24090.17772210283093826No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23770.17536132769993368No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23660.17454981124865085No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23440.1729267783460852No Hit
CACCTAGCCTGGTACCAGCAGAAAGCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATCCACCAGGGCCG22590.16665596940435431No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22470.16577067873022758No Hit
CTGCTACTCTGGCTCCCAGAAACCACTGGAGAAATAGTGATGACGCAGTC22210.16385254893628637No Hit
CTCCCAGGCTCCTCATTTATGGTGCATCCACCAGGGCCGCTGGTGTCCCA21830.16104912846821845No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21430.1580981595544627No Hit
GCCCTGGTGGATGCACCATAAATGAGGAGCCTGGGAGCCTGGCCAGCTTTCTGCTGGTACCAGGCTAGGTGGCTG21300.1571390946574921No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT20790.15337660929245356No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG20250.14939280125888335No Hit
GCCTGGTACCAGCAGAAAGCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATCCACCAGGGCCGCTGGTGTC20210.14909770436750777No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20040.1478435425791616No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19910.14688447768219098No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT19240.14194160475165016No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19030.1403923460719284No Hit
GACTATTAGCAGCCACCTAGCCTGGTACCAGCAGAAAGCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATC18900.1394332811749578No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18790.13862176472367496No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18620.13736760293532876No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18410.13581834425560704No Hit
GGCTAGGTGGCTGCTAATAGTCTCACTGGCCCTGCAGAAGAGGGTGGCTC17820.13146566510781735No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17810.13139189088497344No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACTGGAGAAATAGTGATGACGCAGTCTC17680.13043282598800282No Hit
CTCTTCTGCAGGGCCAGTGAGACTATTAGCAGCCACCTAGCCTGGTACCAGCAGAAAGCTGGCCAGGCTCCCAGG17620.12999018065093948No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17610.12991640642809557No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17570.12962130953672002No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17370.12814582507984215No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17300.1276294055199349No Hit
GTCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGAGTTCAGTCTCTC16690.12312917792645743No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16680.12305540370361352No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16610.1225389841437063No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16300.1202519832355456No Hit
GCTGATGGAGAGACTGAACTCTGTCCCAGACCCACTGCCGCTGAACCTGG16230.11973556367563835No Hit
GCAGAAAGCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATCCACCAGGGCCGCTGGTGTCCCAGCCAGGTT16160.1192191441157311No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAC15920.11744856276747766No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15840.11685836898472651No Hit
GCCTGGGAGCCTGGCCAGCTTTCTGCTGGTACCAGGCTAGGTGGCTGCTA15760.11626817520197538No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15700.11582552986491201No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15690.11575175564206813No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC15640.11538288452784867No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15510.11442381963087805No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15500.11435004540803417No Hit
ACCTTGGTCCCTCCGCCGAATGAGAGCGGAGGCCAATCATTATACTGCTG15370.11339098051106355No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15010.1107351084886834No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14990.11058756004299562No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTGAGACTATTAGCAGCCACCTAGC14970.11044001159730783No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14810.10925962403180556No Hit
CTGGTGTCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGAGTTCAGT14480.10682507467795707No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACTGGAGAAATAGTGATGACG14230.10498071910685974No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14170.1045380737697964No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14140.10431675110126472No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACC14090.10394787998704526No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14030.10350523464998189No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14010.1033576862042941No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13960.10298881509007464No Hit
CCTCATTTATGGTGCATCCACCAGGGCCGCTGGTGTCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGAGTT13800.10180842752457234No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13800.10180842752457234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG901.8189894E-1242.22353740
GGTATCA10150.041.6223451
GTGGTAT2350.040.593931
ATGCCGT955.456968E-1240.22241644
ATCTCGT1100.037.529937
GTATCAA25300.035.95521
CGTATGC1102.7284841E-1134.59441441
ACCGTCC502.0035527E-434.0711638
TATCAAC28000.032.1230552
TGGTATC3100.031.8718472
ACTATAG553.5059394E-430.9726433
ATCAACG29100.030.908783
TCAACGC29500.030.489684
AATCGAG1103.916956E-430.38968870
TCTCCGA1351.0913936E-1130.28771211
TATATAC902.2275708E-730.284363
ATATACC902.2275708E-730.284364
TAGGGCG450.004915514530.284365
AATGGTA7750.030.19362870
CAACGCA30050.029.9316315
Produced by FastQC (version 0.11.5)