FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355487
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT82640.6096701775819318No Hit
GTATAATGATTGGCCTCCGCTCTCATTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT72320.5335351796070342No Hit
TCATTATACTGCTGACAATAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGAGAGACTGAACTCTGTC69760.5146489785589976No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA56560.41726700440505887No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55680.4107748727947963No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52980.3908558326269452No Hit
GTCTGGGACAGAGTTCAGTCTCTCCATCAGCAGCCTGCAGTCTGAAGATT50180.3701990502306551No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49910.36820714621387No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47550.35079642962271124No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTGAGACTATTAGCAG42820.31590122221754985No Hit
GTCCCAGACCCACTGCCGCTGAACCTGGCTGGGACACCAGCGGCCCTGGT41560.3066056701392193No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41040.3027694105513369No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40630.29974466741473726No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT37200.274440108979282No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36490.26920213915736557No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35550.2622673622100396No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35210.2597590386333473No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34910.2575458119480305No Hit
GAGTTCAGTCTCTCCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTATTGTCAGCAGTATAATGATTGG33700.2486191309839194No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAATGAGAGCGGAGGCCAATCATTATACTG33610.24795516297832437No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTGAGACTATTAGCAGCCACCTAGCCTGGTA31300.2309133175013851No Hit
CACCAGGGCCGCTGGTGTCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGAGTTCAGTCTCTCCATCAGCAG31160.2298804783815706No Hit
CTCCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTATTGTCAGC31130.22965915571303894No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30890.22788857436478552No Hit
CATTTATGGTGCATCCACCAGGGCCGCTGGTGTCCCAGCCAGGTTCAGCG29540.21792905428085993No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28940.21350260091022635No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28750.2121008906761924No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT28670.21151069689344126No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC28220.20819085686546607No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27490.20280533859786185No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26390.19469017408503364No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26370.19454262563934588No Hit
CTTCTGCAGGGCCAGTGAGACTATTAGCAGCCACCTAGCCTGGTACCAGCAGAAAGCTGGCCAGGCTCCCAGGCT26320.1941737545251264No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26290.19395243185659472No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26190.1932146896281558No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT25390.18731275180064433No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGCCAGCTTTCTGCTGGTACCAGGC23490.17329564946030468No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23200.17115619699783177No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC23010.1697544867637978No Hit
CTGCTACTCTGGCTCCCAGAAACCACTGGAGAAATAGTGATGACGCAGTC22470.16577067873022758No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22440.1655493560616959No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22000.16230329025656462No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21890.1614917738052818No Hit
CACCTAGCCTGGTACCAGCAGAAAGCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATCCACCAGGGCCG20060.14799109102484936No Hit
CTCCCAGGCTCCTCATTTATGGTGCATCCACCAGGGCCGCTGGTGTCCCA20020.14769599413347378No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19630.14481879944256196No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19570.14437615410549862No Hit
GCCCTGGTGGATGCACCATAAATGAGGAGCCTGGGAGCCTGGCCAGCTTTCTGCTGGTACCAGGCTAGGTGGCTG19270.14216292742018183No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19210.14172028208311846No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG18930.13965460384348946No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18830.1389168616150505No Hit
GCCTGGTACCAGCAGAAAGCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATCCACCAGGGCCGCTGGTGTC18780.13854799050083108No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT18720.1381053451637677No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18630.13744137715817267No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18550.13685118337542151No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18410.13581834425560704No Hit
GCTGATGGAGAGACTGAACTCTGTCCCAGACCCACTGCCGCTGAACCTGG18260.13471173091294864No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17980.13264605267331964No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17850.13168698777634902No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17710.13065414865653452No Hit
GACTATTAGCAGCCACCTAGCCTGGTACCAGCAGAAAGCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATC17190.12681788906865207No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16910.12475221082902307No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16810.12401446860058414No Hit
CTCTTCTGCAGGGCCAGTGAGACTATTAGCAGCCACCTAGCCTGGTACCAGCAGAAAGCTGGCCAGGCTCCCAGG16530.12194879036095513No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16420.12113727390967231No Hit
GGCTAGGTGGCTGCTAATAGTCTCACTGGCCCTGCAGAAGAGGGTGGCTC16380.12084217701829675No Hit
GACTGAACTCTGTCCCAGACCCACTGCCGCTGAACCTGGCTGGGACACCA16280.1201044347898578No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACTGGAGAAATAGTGATGACGCAGTCTC16260.11995688634417004No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16150.11914536989288721No Hit
GCCTGGGAGCCTGGCCAGCTTTCTGCTGGTACCAGGCTAGGTGGCTGCTA16120.11892404722435553No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT15920.11744856276747766No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15820.11671082053903875No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15810.11663704631619484No Hit
GTCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGAGTTCAGTCTCTC15500.11435004540803417No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15150.11176794760849791No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15140.11169417338565402No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14900.10992359203740058No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14800.10918584980896165No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAC14690.10837433335767882No Hit
GCAGAAAGCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATCCACCAGGGCCGCTGGTGTCCCAGCCAGGTT14620.10785791379777157No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14590.10763659112923989No Hit
ACCTTGGTCCCTCCGCCGAATGAGAGCGGAGGCCAATCATTATACTGCTG14570.10748904268355211No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14570.10748904268355211No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14490.10689884890080098No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14220.10490694488401585No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14210.10483317066117197No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14140.10431675110126472No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTGAGACTATTAGCAGCCACCTAGC14110.10409542843273303No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13830.10202975019310402No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13820.10195597597026014No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13730.10129200796466512No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13690.10099691107328952No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACC13680.10092313685044563No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13630.10055426573622617No Hit
GCAGTATAATGATTGGCCTCCGCTCTCATTCGGCGGAGGGACCAAGGTGG13600.1003329430676945No Hit
CTGGTGTCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGAGTTCAGT13590.1002591688448506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)