FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098387
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTGCCTCAGTCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAGACGAACTGTGG55740.5074714103499041No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40940.3727283735149815No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA34870.3174655198941721No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33640.30626728102208056No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA31310.28505435697982584No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28620.26056389960915416No Hit
GTGTATTACTGTCAGCAGTATGGTGCCTCAGTCACTTTCGGCGGAGGGAC25990.2366196977932186No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25450.23170339780059307No Hit
CATCTATGGTGCGTCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA24820.22596771447586325No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23260.21176507005272277No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23240.21158298486781071No Hit
GTATTACTGTGCTAGGACTGGTCCTTCCCATAGAAGCAGCTGGTACAATTTCTTTGACTACTGGGGCCAGGGAAC22820.20775919598465753No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCTGGTACCAGGCTAAGAAGCTGCTGCTAACACTCGGA21980.20011161821835108No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20760.18900442193871558No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20600.18754774045941913No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19900.18117475898749713No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19740.17971807750820065No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT19730.17962703491574464No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19540.1778972256590801No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19540.1778972256590801No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19390.17653158677223965No Hit
CTCCTGCAGGGCCAGTCCGAGTGTTAGCAGCAGCTTCTTAGCCTGGTACC19240.17516594788539924No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19040.17334509603627865No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17600.1602349627226105No Hit
GTATGGTGCCTCAGTCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAGACGAACTGTGGCTGCACCATCTGTCTT17480.15914245161313817No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17410.15850515346594599No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT17410.15850515346594599No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCCGA16440.14967402199771118No Hit
TCTATGGTGCGTCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT16280.14821734051841473No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCCGAGTGTTAGCAG16120.14676065903911828No Hit
GTCCGAGTGTTAGCAGCAGCTTCTTAGCCTGGTACCAGCAGAGACCTGGC15890.14466667941262962No Hit
CAGTAATACAGGGCCGTGTCCTCAGATCTCAGGCTTCTCACCTCCATGTA14980.13638180349913098No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14690.13374156831790618No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGACCTCCACCTTGGTCC14450.13155654609896147No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA14300.13019090721212104No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14280.13000882202720898No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14150.12882526832528063No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13640.12418209611002315No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA13630.12409105351756712No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13350.12154186092879832No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13080.11908371093248556No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13050.11881058315511746No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12930.1177180720456451No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12800.11653451834371675No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT12330.1122555164982834No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12300.1119823887209153No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12210.11116300538881105No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11990.10916006835477843No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11770.1071571313207458No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11750.10697504613583374No Hit
GTGCAGCCACAGTTCGTCTGACCTCCACCTTGGTCCCTCCGCCGAAAGTG11660.10615566280372947No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11310.10296917206776847No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11290.10278708688285641No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAAGTTTCCTGCAAGGCATCTGGATACACCTTCACCAG11220.1021497887356642No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC11200.10196770355075215No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11150.10151249058847199No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC11090.10096623503373582No Hit
GGTCTCTGCTGGTACCAGGCTAAGAAGCTGCTGCTAACACTCGGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC11000.10014685170163158No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATGG502.7182652E-454.5595270
GACGACT200.006555714653.2847359
GGTATCA9850.046.326081
GTATCAA29200.041.2835851
CGTATGC2800.040.744341
ACTAGCG3150.040.07370827
TAGCGCT3200.039.49799729
TCGTATG2800.039.46766740
CTAGCGC3250.038.86726428
ACACTAC451.0799947E-437.8454256
ACGAGAC3350.037.6229122
ATGCCGT2800.037.17945544
CGCTCAT3150.036.9508932
GTATGCC3000.036.92001342
CTTATAC3450.036.5207441
GCGCTCA3500.036.16861731
ATCTCGT2950.036.1383637
TGCGGAT400.00208565936.074865
GAGACTA3500.036.03018624
CGAGACT3500.036.0219823
Produced by FastQC (version 0.11.5)