FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289561
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC81940.6354100348878418No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC72830.5647658389172749No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGACCACTGTTGTCTGTTGAGTAA66410.5149814549292356No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48980.3798191787747924No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43280.3356180901872808No Hit
CCCCATGACCACTGTTGTCTGTTGAGTAACAGTAATAATCAGCTTCATCC42080.3263125978530678No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGTCAAAA41960.32538204861964654No Hit
TCTATGAGGACAGCAAACGACCCTCCGGGATCCCTCAGAGATTCTCTGGC41800.32414131630841814No Hit
GACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGACCACTGTTGTCTGTTGAGTAACAGTAATAAT38570.2990940327754949No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38550.29893894123659137No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA37660.2920373677553834No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG36820.28552352312143436No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36620.28397260773239885No Hit
GTGTTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCTCAGAG36220.2808707769543279No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTC36150.28032795656816545No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT34030.2638882534443892No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG33250.2578396834271508No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT32810.25442766957127266No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG32440.251558476101557No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32070.2486892826318414No Hit
GATTATTACTGTTACTCAACAGACAACAGTGGTCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTTGGT31430.2437263533869278No Hit
CCCCTGTGTTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCT30010.23271485412477577No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29420.22813965372712108No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29320.22736419603260333No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29250.22682137564644092No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC29240.22674382987698913No Hit
ACACAGGGGCCTGGCCTGACTTCTGCTGATACCAATAAGCATATTTTTTT28920.2242623652545324No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG28460.22069525985975075No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27880.2161976052315478No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC27840.21588742215374068No Hit
GTCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCCC27350.21208767945060372No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27050.2097613063670505No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26790.20774511636130433No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25750.1996803563383198No Hit
GGCCAGGCCCCTGTGTTGGTCATCTATGAGGACAGCAAACGACCCTCCGG25520.197896803640929No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC23520.18238764975057403No Hit
GTAATAATCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGG23350.18106937166989387No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23220.1800612766670208No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23110.17920827320305127No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC22240.17246179126074687No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC22180.17199651664403623No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21830.1692824147132241No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21410.16602549239624959No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21270.16493985162392474No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20340.1577280950649097No Hit
GCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGATTATTACTGTTACTCAA20160.15633227121477775No Hit
GCTCTGGAGATGCATTGTCAAAAAAATATGCTTATTGGTATCAGCAGAAG20080.15571190505916355No Hit
GACCAACACAGGGGCCTGGCCTGACTTCTGCTGATACCAATAAGCATATT20040.15540172198135643No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC19540.1515244335087677No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18980.1471818704194683No Hit
GGATCACCTGCTCTGGAGATGCATTGTCAAAAAAATATGCTTATTGGTATCAGCAGAAGTCAGGCCAGGCCC18910.1466390500333059No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTGAGGGATCCCGGAGGGTCGTTTG18910.1466390500333059No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18300.14190875809674763No Hit
GACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGATTATTACTGTTACT18110.14043538847716394No Hit
CCTCATAGATGACCAACACAGGGGCCTGGCCTGACTTCTGCTGATACCAATAAGCATATTTTTTTGACAATGCAT17820.13818656116306247No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17770.13779883231580362No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17540.13601527961841278No Hit
GGAGATGCATTGTCAAAAAAATATGCTTATTGGTATCAGCAGAAGTCAGG17400.13492963884608794No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGACCA17330.1343868184599255No Hit
ACAGACAACAGTGGTCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCCCAAGGCTGCCCC16940.1313625334513063No Hit
CTGTTGTCTGTTGAGTAACAGTAATAATCAGCTTCATCCTCCACCTGGGC16710.12957898075391547No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16650.12911370613720483No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA16390.12709751613145867No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16360.12686487882310338No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCC16230.12585678382023027No Hit
GCAAACGACCCTCCGGGATCCCTCAGAGATTCTCTGGCTCCAGCTCAGGG16100.12484868881735722No Hit
CCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGATTATTACTGT15990.1239956853533877No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15920.12345286496722528No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15760.12221213265599688No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15670.1215142207309309No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15590.12089385457531672No Hit
GGCCTGGCCTGACTTCTGCTGATACCAATAAGCATATTTTTTTGACAATGCATCTCCAGAGCAGGTGATCCTGGC15560.1206612172669614No Hit
AGTCAGGCCAGGCCCCTGTGTTGGTCATCTATGAGGACAGCAAACGACCC15500.12019594265025074No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15440.1197306680335401No Hit
GCTTGGTCCCTCCGCCGAACACCCCATGACCACTGTTGTCTGTTGAGTAACAGTAATAATCAGCTTCATCCTCCA15130.11732674918053508No Hit
GTCCCTGAGCTGGAGCCAGAGAATCTCTGAGGGATCCCGGAGGGTCGTTT14980.11616356263875846No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14930.1157758337914996No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14870.11531055917478894No Hit
GGATGAAGCTGATTATTACTGTTACTCAACAGACAACAGTGGTCATGGGGTGTTCGGCGGAGGGACCAAGCTGAC14870.11531055917478894No Hit
CAACAGTGGTCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCCCAAGGCTGCCCCCTCGGT14640.11352700647739812No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14470.11220872839671796No Hit
GTATCAACGCAGAGTACGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTC14460.1121311826272662No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC14360.11135572493274842No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG13840.10732334492125616No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC13700.1062377041489313No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13670.10600506684057599No Hit
GGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGTCAAAAAAATA13630.10569488376276888No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT13610.10553979222386534No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13600.10546224645441357No Hit
CAGTAATAATCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGT13540.1049969718377029No Hit
GCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCC13450.10429905991263694No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13450.10429905991263694No Hit
GGACCAAGCTGACCGTCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG13380.1037562395264745No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG13330.10336851067921564No Hit
TTATTACTGTTACTCAACAGACAACAGTGGTCATGGGGTGTTCGGCGGAG13320.10329096490976387No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13270.10290323606250498No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT13140.10189514105963192No Hit
GTTGTCTGTTGAGTAACAGTAATAATCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGGCCATTGT13120.10174004952072838No Hit
CCACTGTTGTCTGTTGAGTAACAGTAATAATCAGCTTCATCCTCCACCTG13030.1010421375956624No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTG13010.10088704605675884No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12960.10049931720949998No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGG12920.10018913413169286No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12920.10018913413169286No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT150.002364139368.93709639
ATCGCGT208.6566055E-568.76980637
TATCGCG252.6221582E-454.96420736
CGAGACG400.00720249552.0294770
GACCGAT1550.050.58910426
CGTATGC1000.048.39649241
ACCGATC1650.047.5415527
TCGTATG900.046.0339340
AGACCGA1850.044.21115525
CCGATCA1800.043.6017628
ACGAGAC1900.041.2380522
TATCTCG1100.040.59856436
GTATGCC1450.040.58018542
TCTCCGA1850.040.51117711
ATGCCGT1200.040.4689644
CACGAGA1950.040.1759921
GAGACCG2050.039.88858824
TCAGTAT2000.039.3562432
TCTCGTA1050.039.33706738
CCCACGA2000.039.1715919
Produced by FastQC (version 0.11.5)