FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289561
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC79350.6153256805998321No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC71580.5550726177358031No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGACCACTGTTGTCTGTTGAGTAA61150.4741923801976021No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG49940.38726357264216277No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39390.3054527858705405No Hit
GTGTTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCTCAGAG37530.29102927275251034No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGTCAAAA37340.2895559031329266No Hit
CCCCATGACCACTGTTGTCTGTTGAGTAACAGTAATAATCAGCTTCATCC36790.28529088581307904No Hit
GACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGACCACTGTTGTCTGTTGAGTAACAGTAATAAT36490.2829645127295258No Hit
TCTATGAGGACAGCAAACGACCCTCCGGGATCCCTCAGAGATTCTCTGGC36090.2798626819514548No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34380.2666023553752013No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG34370.26652480960574954No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34180.2650514399861658No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33420.25915796150783094No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG31840.24690572993445056No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31740.2461302722399328No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31180.24178770915063344No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTC31060.24085715991721213No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG29630.22976811488560836No Hit
GATTATTACTGTTACTCAACAGACAACAGTGGTCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTTGGT29340.2275192875715069No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29080.22550309756576076No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC28590.2217033548626238No Hit
CCCCTGTGTTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCT28030.2173607917733244No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27630.2142589609952534No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC27050.2097613063670505No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26140.202704641346939No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25810.2001456309550304No Hit
ACACAGGGGCCTGGCCTGACTTCTGCTGATACCAATAAGCATATTTTTTT25360.19665607132970056No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25260.19588061363518283No Hit
GTCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCCC25250.19580306786573104No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25210.19549288478792393No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25030.194097060937792No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24200.1876607620732947No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23280.18052655128373143No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC22950.17796754089182287No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22470.17424534395813768No Hit
GTAATAATCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGG22320.17308215741636107No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22010.17067823856335604No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21690.16819677394089927No Hit
GGCCAGGCCCCTGTGTTGGTCATCTATGAGGACAGCAAACGACCCTCCGG21080.16346648200434102No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20310.15749545775655438No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20290.15734036621765082No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20090.1557894508286153No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC19960.15478135582574226No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19890.15423853543957983No Hit
GACCAACACAGGGGCCTGGCCTGACTTCTGCTGATACCAATAAGCATATT19450.15082652158370174No Hit
GCTCTGGAGATGCATTGTCAAAAAAATATGCTTATTGGTATCAGCAGAAG18850.14617377541659526No Hit
GCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGATTATTACTGTTACTCAA18550.14384740233304202No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTGAGGGATCCCGGAGGGTCGTTTG18300.14190875809674763No Hit
GTATCAACGCAGAGTACGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTC18230.14136593771058523No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17950.13919465616593554No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17550.13609282538786455No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17260.1338439980737631No Hit
GGATCACCTGCTCTGGAGATGCATTGTCAAAAAAATATGCTTATTGGTATCAGCAGAAGTCAGGCCAGGCCC17190.13330117768760066No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC17190.13330117768760066No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA17050.13221553691527582No Hit
CTGTTGTCTGTTGAGTAACAGTAATAATCAGCTTCATCCTCCACCTGGGC16810.13035443844843322No Hit
CCTCATAGATGACCAACACAGGGGCCTGGCCTGACTTCTGCTGATACCAATAAGCATATTTTTTTGACAATGCAT16170.12539150920351966No Hit
GACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGATTATTACTGTTACT16140.12515887189516434No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16100.12484868881735722No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16060.12453850573955012No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16030.1243058684311948No Hit
CCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGATTATTACTGT15740.12205704111709333No Hit
GGAGATGCATTGTCAAAAAAATATGCTTATTGGTATCAGCAGAAGTCAGG15660.12143667496147914No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGACCA15650.12135912919202738No Hit
ACAGACAACAGTGGTCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCCCAAGGCTGCCCC15650.12135912919202738No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC15500.12019594265025074No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG14780.11461264724972296No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14690.11391473532465698No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14660.11368209801630166No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG14420.11182099954945908No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14280.11073535877713424No Hit
GGCCTGGCCTGACTTCTGCTGATACCAATAAGCATATTTTTTTGACAATGCATCTCCAGAGCAGGTGATCCTGGC14160.10980480954371294No Hit
GCAAACGACCCTCCGGGATCCCTCAGAGATTCTCTGGCTCCAGCTCAGGG13970.1083314399241292No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13960.10825389415467744No Hit
GGATGAAGCTGATTATTACTGTTACTCAACAGACAACAGTGGTCATGGGGTGTTCGGCGGAGGGACCAAGCTGAC13900.10778861953796678No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC13890.10771107376851502No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC13850.10740089069070792No Hit
GCTTGGTCCCTCCGCCGAACACCCCATGACCACTGTTGTCTGTTGAGTAACAGTAATAATCAGCTTCATCCTCCA13790.10693561607399728No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC13670.10600506684057599No Hit
AGTCAGGCCAGGCCCCTGTGTTGGTCATCTATGAGGACAGCAAACGACCC13500.1046867887598958No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13470.10445415145154048No Hit
GTCCCTGAGCTGGAGCCAGAGAATCTCTGAGGGATCCCGGAGGGTCGTTT13430.10414396837373338No Hit
CAACAGTGGTCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCCCAAGGCTGCCCCCTCGGT13370.10367869375702274No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13330.10336851067921564No Hit
GTTGTCTGTTGAGTAACAGTAATAATCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGGCCATTGT13320.10329096490976387No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13290.10305832760140854No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTG13280.10298078183195677No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13170.10212777836798724No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13110.10166250375127661No Hit
TTATTACTGTTACTCAACAGACAACAGTGGTCATGGGGTGTTCGGCGGAG12910.1001115883622411No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC209.110848E-568.063183
CTTAACG200.00766714651.21623230
CGGTAAG200.007759561851.0612520
CCTAACG306.7360484E-445.396582
TAACGCC306.756679E-445.3684164
GTGGTAT3350.041.6991231
GTATCAA28100.040.497481
TATCAAC31400.035.673912
ATCAACG33250.033.7757153
TCAACGC33450.033.5685584
CAACGCA34250.032.883825
AACGCAG34450.032.7916876
CCGCAGT450.00493692530.25734517
CGCAGAG37350.030.245618
GCACCGA2500.029.9431537
AGAGTAC38250.029.53051211
GCAGAGT38800.029.2029939
TCGCTGC2450.029.183522
TACTGTC704.232289E-529.165417
ATACGTA1052.6699126E-829.16201611
Produced by FastQC (version 0.11.5)