FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004687077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769648
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33100.43006673180466914No Hit
GTCTATTAGTAAGTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAAACTCCTGATCTATGAGGCGT29770.3868001995717523No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29370.38160301852275325No Hit
CTAATAGACTGACTGGCCCGGCAAGTGATGATGACTCTGTCTCCTACAGA29190.3792642870507037No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27770.36081429432675716No Hit
GTAATAGACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA25990.3376868386587115No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25740.3344386005030871No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC24320.3159886077791406No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23470.30494459805001767No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22630.29403051784711975No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCA21850.2838960148015716No Hit
GTCTATTACTGTGCGACTTTTCTGAAGAACACTCTAACAATGGCTGGTAG21490.2792185518574725No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20550.2670051763923248No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19790.2571305323992267No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17940.23309357004760617No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17930.23296364052138122No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17930.23296364052138122No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17770.2308847681017816No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17540.22789638899860715No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17430.2264671642101324No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16390.21295449348273499No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14920.19385485312766354No Hit
GTAATATATTGTTGGCAGTAATAAGTTGCAAAATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGCC14660.1904766854458142No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14560.18917739018356444No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGCCG14450.1877481653950897No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13660.17748373282331664No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13560.17618443756106686No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13550.1760545080348419No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13530.17579464898239194No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT13320.17306612893166748No Hit
GTAGGAGACAGAGTCATCATCACTTGCCGGGCCAGTCAGTCTATTAGTAAGTGGTTGGCCTGGTATCAGCAGAAG13010.1690383136186932No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12060.15669500862732055No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11950.15526578383884582No Hit
ATATATTACTTATTCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCAT11950.15526578383884582No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11800.15331684094547118No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11750.1526671933143463No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11610.15084817994719663No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11500.14941895515872192No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGTG11120.14448163316217283No Hit
GTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGCCGGGCCAGTCAGT11040.14344219695237304No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10870.14123339500654844No Hit
ATTAGTAAGTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAA10670.13863480448204896No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10550.13707565016734924No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAGGATTTTGCAACTTA10520.1366858615886743No Hit
GGTATCAGCAGAAGCCAGGGAAAGCCCCTAAACTCCTGATCTATGAGGCGTCTAGTTTAGAAGGTGGGGTCCCAT10450.13577635490509946No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10330.13421720059039977No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10330.13421720059039977No Hit
GAGTGAATTCTGCCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCA10320.13408727106417478No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCC10310.1339573415379498No Hit
ATCTTGATGGGACCCCACCTTCTAAACTAGACGCCTCATAGATCAGGAGTTTAGGGGCTTTCCCTGGCTTCTG10250.13317776438059994No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10240.133047834854375No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGGCCAACC10230.13291790532815No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10190.1323981872232501No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10160.13200839864457517No Hit
CTTACTAATAGACTGACTGGCCCGGCAAGTGATGATGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA10140.13174853959212524No Hit
AAACTAGACGCCTCATAGATCAGGAGTTTAGGGGCTTTCCCTGGCTTCTG10070.1308390329085504No Hit
GTAATAAGTTGCAAAATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGCCCCAGATCCACTGCCG10040.13044924432987548No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9990.1297995966987506No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9980.12966966717252562No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9960.12940980812007566No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9950.1292798785938507No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9910.1287601604889508No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9900.1286302309627258No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9830.127720724279151No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9820.127590794752926No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC9810.12746086522670103No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9740.1265513585431262No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9640.12525206328087646No Hit
GTTTAGAAGGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGGCAGAATTCACTCTCACCATCAGCAGC9600.12473234517597655No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9310.12096438891545226No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9250.1201848117581024No Hit
GGCCAAAAGTGTACGGAGAATAAGTAATATATTGTTGGCAGTAATAAGTTGCAAAATCCTCAGGCTGCAGGCTG9250.1201848117581024No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9040.11745629170737792No Hit
GTTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGGCCAACCACTTACTA9020.11719643265492798No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8970.11654678502380308No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8940.11615699644512816No Hit
GCTGATACCAGGCCAACCACTTACTAATAGACTGACTGGCCCGGCAAGTGATGATGACTCTGTCTCCTACAGATG8850.11498763070910338No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8780.11407812402552855No Hit
CTTCTAAACTAGACGCCTCATAGATCAGGAGTTTAGGGGCTTTCCCTGGC8700.11303868781572875No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8670.11264889923705383No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8650.11238904018460387No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8550.11108974492235411No Hit
CCTCATAGATCAGGAGTTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGGCCAACCACTTACTAATAGACTGA8500.11044009729122924No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8440.10966052013387938No Hit
GGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC8410.10927073155520446No Hit
CTCCTGATCTATGAGGCGTCTAGTTTAGAAGGTGGGGTCCCATCAAGATT8310.10797143629295469No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8310.10797143629295469No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC8260.10732178866182983No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG8190.106412281978255No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8040.10446333908488036No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7950.10329397334885558No Hit
GCTGATGGTGAGAGTGAATTCTGCCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCACCTTCTAAACTAGA7800.10134503045548095No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7800.10134503045548095No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG7770.10095524187680603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)