FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769648
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31080.4038209675072241No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29650.3852410452570526No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28270.36731077063800593No Hit
GTCTATTAGTAAGTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAAACTCCTGATCTATGAGGCGT27680.3596449285907324No Hit
CTAATAGACTGACTGGCCCGGCAAGTGATGATGACTCTGTCTCCTACAGA25660.3333991642932873No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23560.30611396378604244No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC22980.29857805126499387No Hit
GTAATAGACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA22800.2962393197929443No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22240.28896326632434566No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCA21920.2848055214851465No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20790.27012348502172423No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20450.26570588113007504No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19510.2534925056649273No Hit
GTCTATTACTGTGCGACTTTTCTGAAGAACACTCTAACAATGGCTGGTAG18960.2463463817225537No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18740.24348793214560424No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17970.2334833586262811No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17790.23114462715423156No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17500.22737667089370728No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16580.2154231544810095No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16120.20944639627466063No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15220.1977527389144128No Hit
GTAATATATTGTTGGCAGTAATAAGTTGCAAAATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGCC15140.19671330270461304No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14260.18527950439681518No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13860.18008232334781615No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13840.1798224642953662No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGCCG13190.17137704509074278No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13160.17098725651206786No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13100.170207679354718No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12750.16566014593684386No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT12560.16319148493856933No Hit
GTAGGAGACAGAGTCATCATCACTTGCCGGGCCAGTCAGTCTATTAGTAAGTGGTTGGCCTGGTATCAGCAGAAG12270.15942352867804505No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12150.15786437436334533No Hit
GAGTGAATTCTGCCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCA12090.15708479720599547No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11980.15565557241752073No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGTG11710.1521474752094464No Hit
ATATATTACTTATTCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCAT11680.15175768663077147No Hit
GTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGCCGGGCCAGTCAGT11580.1504583913685217No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11530.14980874373739683No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11200.14552106937197265No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11010.1430524083736981No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10930.1420129721638983No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10780.14006402927052367No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10580.13746543874602415No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10460.13590628443132444No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10390.1349967777477496No Hit
ATCTTGATGGGACCCCACCTTCTAAACTAGACGCCTCATAGATCAGGAGTTTAGGGGCTTTCCCTGGCTTCTG10330.13421720059039977No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10290.13369748248549987No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10210.13265804627570008No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10150.13187846911835022No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10000.12992952622497558No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9860.12811051285782593No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC9810.12746086522670103No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGGCCAACC9670.12564185185955137No Hit
ATTAGTAAGTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAA9600.12473234517597655No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9540.12395276801862669No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9370.12174396607280212No Hit
GGTATCAGCAGAAGCCAGGGAAAGCCCCTAAACTCCTGATCTATGAGGCGTCTAGTTTAGAAGGTGGGGTCCCAT9230.11992495270565245No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9210.11966509365320249No Hit
GTTTAGAAGGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGGCAGAATTCACTCTCACCATCAGCAGC9170.11914537554830261No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9020.11719643265492798No Hit
CTCCTGATCTATGAGGCGTCTAGTTTAGAAGGTGGGGTCCCATCAAGATT8920.1158971373926782No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCC8910.11576720786645324No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8900.11563727834022826No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8880.11537741928777832No Hit
GTAATAAGTTGCAAAATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGCCCCAGATCCACTGCCG8870.11524748976155333No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8870.11524748976155333No Hit
GGCCAAAAGTGTACGGAGAATAAGTAATATATTGTTGGCAGTAATAAGTTGCAAAATCCTCAGGCTGCAGGCTG8810.11446791260420348No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAGGATTTTGCAACTTA8790.11420805355175352No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8720.1132985468681787No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8660.11251896971082884No Hit
GTTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGGCCAACCACTTACTA8660.11251896971082884No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG8500.11044009729122924No Hit
CTTACTAATAGACTGACTGGCCCGGCAAGTGATGATGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA8400.1091408020289795No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT8400.1091408020289795No Hit
GCTGATACCAGGCCAACCACTTACTAATAGACTGACTGGCCCGGCAAGTGATGATGACTCTGTCTCCTACAGATG8380.10888094297652952No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8290.10771157724050474No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8290.10771157724050474No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8260.10732178866182983No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGCCCCAGATCCACTGCC8200.10654221150447997No Hit
AAACTAGACGCCTCATAGATCAGGAGTTTAGGGGCTTTCCCTGGCTTCTG8150.10589256387335509No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8110.10537284576845518No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7940.1031640438226306No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7910.10277425524395568No Hit
GCTGATGGTGAGAGTGAATTCTGCCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCACCTTCTAAACTAGA7900.10264432571773069No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7890.10251439619150572No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7860.1021246076128308No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7850.10199467808660584No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7840.10186474856038086No Hit
CTTCTAAACTAGACGCCTCATAGATCAGGAGTTTAGGGGCTTTCCCTGGC7820.1016048895079309No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7780.101085171403031No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7720.10030559424568114No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG7710.10017566471945617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC200.007745048551.08346612
GACGCGA200.007745048551.08346611
GATACAT200.00777696251.0303572
CCGACAT400.00857165249.78500770
GCTTGTA405.4291035E-542.5280651
GTGGTAT1500.038.5587771
TCTGGAG7800.037.44513370
GTATCAA10750.037.3455731
ACGAACC400.00238171835.11067652
TCGTTGA501.8322057E-434.591141
TATCAAC12700.031.8772322
CGACTTT5450.031.86858414
AATAGCG450.003857875531.81972362
GCGACTT5500.031.57886913
TGCGACT5500.030.95967512
AATGGTA3650.030.91671670
ATCAACG13050.030.7615953
ACGGGTA450.004921146730.2756216
GAACACT5650.030.206527
TGTGCGA5650.030.13185510
Produced by FastQC (version 0.11.5)