FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898872
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30480.3390916615491416No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC30390.33809040664299256No Hit
CCCGTAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCG29010.32273783141537393No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCG28950.3220703281446079No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA26780.2979289598519033No Hit
CCCTTACGGGTGCGCAGGCTGAGGATGAGGCCGAGTATTACTGCTTGCTC24930.2773476090032841No Hit
GGCTGAGGATGAGGCCGAGTATTACTGCTTGCTCTCCCATAGTGGTGGTCGGGTGTTCGGCGGAGGGACCAAA24840.2763463540971351No Hit
TCCTCAGCCTGCGCACCCGTAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC24250.2697825719346025No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT24080.26789131266743205No Hit
GTATTACTGCTTGCTCTCCCATAGTGGTGGTCGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCC24070.26778006212230443No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23470.26110502941464414No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22470.249979974901877No Hit
CCCATAGTGGTGGTCGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTA21940.24408369601011046No Hit
CCACTATGGGAGAGCAAGCAGTAATACTCGGCCTCATCCTCAGCCTGCGCACCCGTAAGGGTCAGGGCAGCTTTG21720.2416361840173017No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCGACCACCACTATGGGAGAGCAAGCAGTAATACTCGG21470.2388549203891099No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21440.2385211687537269No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC21330.2372974127573225No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC20890.23240238877170497No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20460.2276186153312151No Hit
CAGTAATACTCGGCCTCATCCTCAGCCTGCGCACCCGTAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC20260.22539360442866171No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20240.22517110333840637No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19990.22238983971021456No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18550.20636976121182993No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTACGGGTG18450.20525725576055323No Hit
CTATGGGAGAGCAAGCAGTAATACTCGGCCTCATCCTCAGCCTGCGCACCCGTAAGGGTCAGGGCAGCTTTGCCC18210.20258724267748912No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17810.19813722087238228No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17350.1930196957965094No Hit
TTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTACGGGTGCGCAGG16970.18879217508165788No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAC16610.18478715545706173No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16400.18245089400938064No Hit
CTCCCATAGTGGTGGTCGGGTGTTCGGCGGAGGGACCAAACTGACCGTCC16200.18022588310682722No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAAC16140.17955837983606118No Hit
GTAATACTCGGCCTCATCCTCAGCCTGCGCACCCGTAAGGGTCAGGGCAG16110.1792246282006782No Hit
GAGTATTACTGCTTGCTCTCCCATAGTGGTGGTCGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAG15980.17777837111401845No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15850.17633211402735874No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15830.1761096129371034No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA15830.1761096129371034No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15720.17488585694069902No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTCCAG15560.17310584821865627No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15450.1718820922222519No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15400.17132583949661354No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15140.16843332532329408No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC14820.1648733078792086No Hit
GGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGG14650.1629820486120382No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14350.15964453225820804No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14230.15830952571667603No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13880.15441575663720752No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC13250.14740697229416425No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12990.1445144581208448No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12920.1437357043049511No Hit
AAGCTGCCCTGACCCTTACGGGTGCGCAGGCTGAGGATGAGGCCGAGTAT12630.14050943849624864No Hit
GTTTGGTCCCTCCGCCGAACACCCGACCACCACTATGGGAGAGCAAGCAGTAATACTCGGCCTCATCCTCAGC12450.13850692868395056No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12430.13828442759369522No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12400.1379506759583122No Hit
GATTTATGATACAACCTACAAACATTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC12380.13772817486805686No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12350.13739442323267384No Hit
GGATGAGGCCGAGTATTACTGCTTGCTCTCCCATAGTGGTGGTCGGGTGTTCGGCGGAGGGACCAAACTGACCGT12320.13706067159729082No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12260.13639316832652482No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12150.13516941233012042No Hit
GCCCTGACCCTTACGGGTGCGCAGGCTGAGGATGAGGCCGAGTATTACTG12100.13461315960448206No Hit
GTCCAGGAATGTTTGTAGGTTGTATCATAAATCAGTGTCCTGGGGGCTTG11950.132944401427567No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11340.12615811817477907No Hit
CTCTCACCTGTGGCTCCAACACTGGAGCTGTCACCAGTGGTCATTTTTCC10890.12115184364403385No Hit
GCCTCATCCTCAGCCTGCGCACCCGTAAGGGTCAGGGCAGCTTTGCCCCC10730.1193718349219911No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10630.11825932947071441No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10610.11803682838045908No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10390.1155893163876503No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCGACCACCA10360.11525556475226728No Hit
CCTGAGAACCGGGCAGGGGTCCAGGAATGTTTGTAGGTTGTATCATAAATCAGTGTCCTGGGGGCTTGGTCAGA10230.11380930766560755No Hit
ACACTGGAGCTGTCACCAGTGGTCATTTTTCCTACTGGTTCCAGCAGAAG9990.11113929458254346No Hit
GTTTGTAGGTTGTATCATAAATCAGTGTCCTGGGGGCTTGGTCAGACTTCTGCTGGAACCAGTAGGAAAAATGAC9960.11080554294716045No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9950.11069429240203278No Hit
GGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG9750.10846928149947933No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC9740.10835803095435168No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9460.10524301569077689No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9430.10490926405539386No Hit
GGGTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC9360.10413051023950018No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9320.10368550805898949No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9210.1024617520625851No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT451.2499513E-463.84078270
TACGGAC200.00675568552.8806558
GTACGTA452.3557592E-548.98469569
GGTATCA9650.045.307671
GTGGTAT2350.043.6056181
GTATCAA26300.040.0022131
TGGTATC2750.035.982792
GGTACGT551.6062056E-435.374868
TATCAAC29850.035.093252
ATCAACG30450.034.2801553
TCAACGC30550.034.1603434
AACGCAG31200.033.5579836
CAACGCA31050.033.5004235
GTAGTTT900.003796918831.92039170
ACGCAGA33200.031.5364137
CGCAGAG33400.031.4496828
GCAGAGT34100.031.104139
CAGAGTA34200.030.91174110
AGAGTAC34300.030.82161711
GAGTTTA2550.030.8089351
Produced by FastQC (version 0.11.5)