FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828121
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23740.28667308279828674No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAGGTGTTGGTTGTATATGACTTG20790.2510502692239419No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19890.24018229220126044No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAGGTGTTGGTTGTATATGACTTGCAGTAATAAT18990.22931431517857898No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18470.22303503956547416No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17420.2103557330390124No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT17150.20709533993220797No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG16620.20069530901885113No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT16370.19767642651255068No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC16170.19526132050751038No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGGCCATTTGAAGATACA15630.18874053429390147No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15590.18825751309289343No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15570.1880160024923894No Hit
GATTATTACTGCAAGTCATATACAACCAACACCTATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT15440.1864461835891132No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15140.18282352458155268No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14630.17666500426869988No Hit
GTCATATACAACCAACACCTATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC14320.17292158996088736No Hit
GTATATGACTTGCAGTAATAATCAGCCTCGTCTTCAGCCTGGAGCCCAGA14300.17268007936038332No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14250.17207630285912323No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAATTATGTTTCCTGGTACCAACAACACCCAGGCAAAGC14210.1715932816581152No Hit
TCTCTGGGCTCCAGGCTGAAGACGAGGCTGATTATTACTGCAAGTCATAT14130.17062723925609904No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13770.16628004844702646No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13650.16483098484400227No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT13620.16446871894324622No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13600.16422720834274218No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA13540.1635026765412301No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAATTATGTTTCCTGGTACCAACAACA13160.15891397513165345No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12760.15408376312157282No Hit
GTAATAATCAGCCTCGTCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG12410.14985732761275222No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12310.14864977461023207No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG12110.14623466860519174No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12080.1458724027044357No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12000.14490636030241957No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11880.14345729669939536No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11590.1399553929920869No Hit
CCTATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC11490.13874783998956675No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11240.13572895748326633No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11240.13572895748326633No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11040.133313851478226No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10950.13222705377595786No Hit
CCACATAGGTGTTGGTTGTATATGACTTGCAGTAATAATCAGCCTCGTCT10870.13126101137394172No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10820.13065723487268166No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAAACATAATTATAA10540.1272760864656252No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10330.12474022516033285No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTCGCCAGACTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGAT10290.1242572039593248No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10200.12317040625705665No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10010.12087605555226834No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9280.11206091863387115No Hit
GTATATTACTGTGCGAAAGATTTGAGCATGGTGACCCGTAGGTACTTTGA9270.11194016333361911No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT9190.11097412093160298No Hit
GCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCGACACGG8770.1059023983210183No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8680.10481560061875017No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA8620.10409106881723805No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT8500.10264200521421386No Hit
ACCAACACCTATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGG8470.10227973931345782No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC8440.10191747341270177No Hit
ATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC8420.10167596281219773No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC8370.10107218631093765No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC8330.10058916510992959No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGT100.007717686140.9383470
CACGTAA150.001821713573.61629561
AGGGGGG2650.053.18428470
GACGTTA250.00669159953.0065469
TACGCGA200.007565102551.3883938
ATATAGG200.00822561350.311723
TATACCG200.00822561350.311726
TAATCTC2200.044.89370735
GCCTATA307.250485E-444.7215351
TCGTATG2250.044.3996540
CTTGAAA2450.042.7835757
GACGCGT2400.042.00506226
GTAATCT2350.041.94585834
ACGCGTA2450.041.1951627
GCCGTCT2550.041.1782146
CGTATGC2450.040.91982341
TCTCGTA2350.040.81914538
GTATGCC2550.040.79776442
CCGTCTT2700.040.26553347
TAGTAAT2550.039.85968432
Produced by FastQC (version 0.11.5)