FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828121
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25190.3041826013348291No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22930.2768919034778734No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19240.23233319768487937No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19030.22979733637958702No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAGGTGTTGGTTGTATATGACTTG18480.22315579486572615No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC17650.21313310494480878No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAGGTGTTGGTTGTATATGACTTGCAGTAATAAT17570.21216706254279263No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG16570.20009153251759104No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT16280.19658962881028255No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16170.19526132050751038No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16040.19369150160423418No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT15240.18403107758407286No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15150.18294427988180473No Hit
GATTATTACTGCAAGTCATATACAACCAACACCTATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT15140.18282352458155268No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGGCCATTTGAAGATACA14810.17883859967323615No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAATTATGTTTCCTGGTACCAACAACACCCAGGCAAAGC14740.17799331257147202No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14490.17497443006517163No Hit
GTCATATACAACCAACACCTATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC14490.17497443006517163No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14270.1723178134596273No Hit
TCTCTGGGCTCCAGGCTGAAGACGAGGCTGATTATTACTGCAAGTCATAT14080.17002346275483896No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13940.16833288855131073No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13700.16543476134526236No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC13400.16181210233770185No Hit
GTATATGACTTGCAGTAATAATCAGCCTCGTCTTCAGCCTGGAGCCCAGA13130.1585517092308974No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAATTATGTTTCCTGGTACCAACAACA12930.1561366032258571No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12880.155532826724597No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12560.1516686571165325No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA12360.14925355111149216No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12230.14768373220821596No Hit
GTAATAATCAGCCTCGTCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG12090.14599315800468773No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAAACATAATTATAA11890.1435780519996474No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11510.13898935059007078No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG11440.13814406348830666No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11150.1346421597809982No Hit
CCTATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC11040.133313851478226No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10870.13126101137394172No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10800.1304157242721776No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10730.1295704371704135No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10690.12908741596940546No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA10620.12824212886764133No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10580.12775910766663326No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10550.1273968417658772No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10520.12703457586512115No Hit
CCACATAGGTGTTGGTTGTATATGACTTGCAGTAATAATCAGCCTCGTCT10450.12618928876335705No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10440.126068533463105No Hit
GTATATTACTGTGCGAAAGATTTGAGCATGGTGACCCGTAGGTACTTTGA9980.12051378965151227No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9840.11882321544798406No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTCGCCAGACTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGAT9840.11882321544798406No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9750.1177364177457159No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9700.11713264124445583No Hit
GCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCGACACGG9040.10916279142782276No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8910.10759297252454653No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT8710.1051778665195062No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8700.10505711121925418No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8630.10421182411749008No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC8620.10409106881723805No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC8550.10324578171547395No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC8290.10010614390892152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG150.002229549569.96117446
TACGTAT553.624236E-451.46796870
TATACCG200.00824145350.2872356
ATATACC200.0082453950.2811625
CCTATAT200.00825129650.2720532
ATGGCAA1955.456968E-1239.92066270
GTATCAA30600.037.580751
TAAGTAA601.18397926E-437.25580269
CACGTAA400.001896422536.7793461
TGTCAGT800.002291422335.38422870
ATACGTA1151.8189894E-1235.00145311
GAATACG1151.8189894E-1234.9972239
GGTATCA12400.034.3379861
AGTATAA552.0277839E-433.99899765
TCTGGAG5850.033.8720870
GTAGCAA400.002979747733.5430610
AATACGT1203.6379788E-1233.54305610
TACGTAC1203.6379788E-1233.54305612
GTGCTAG1203.6379788E-1233.526851
TATAACT502.2070622E-433.512684
Produced by FastQC (version 0.11.5)