FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477041
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCAT57910.3920676541815698No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49940.33810842082244164No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGTTGATGGTGAGAGTGAAGT47700.3229429650226365No Hit
ATTACATACTACGCAGACTCCGTGAGGGCCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACACTGTATTTG45060.30506939211572326No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA44200.2992469403354409No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT40200.27216576926436026No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39670.2685775140974421No Hit
GTATGTAATAGCACCACTTCCACTAATAGCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAAGT37700.2552400373449349No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34020.23032535995954076No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32160.21773261541148825No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA31690.21455057781063624No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30860.20893123481338705No Hit
GGACAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG30370.20561379135717966No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT28640.1939011848689373No Hit
CATCTATGGTGCATCCACCAGGGCCTCTGGTATCCCAGTCAGGTTCAGTGGGAGTGGGTCTGGGACAGACTTCA28360.19200550289396165No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28120.19038063262969682No Hit
CCATAGATGAGGAGCCTGGGAGACTGGCCAGGTTTCTGCTGGTACCAGGC27070.18327182522353813No Hit
GTATTTGCTAATGAACAGCCTGAGAGCCGAGGACACGGCCCAATATTTCT26960.18252709301908343No Hit
GTCCCAGACCCACTCCCACTGAACCTGACTGGGATACCAGAGGCCCTGGT26900.18212087545301722No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCATCCTCTCTTGCAGGGCCAGTCAGACAATTAGCAG26620.18022519347804158No Hit
GTGTGAGGTGCAGTTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGAAGC26580.17995438176733075No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25310.17135610995226266No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG25040.16952813090496474No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC24620.16668460794250126No Hit
CTGGTATCCCAGTCAGGTTCAGTGGGAGTGGGTCTGGGACAGACTTCACT24390.16512744060591414No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23540.15937269175330948No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23400.15842485076582166No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23280.15761241563368925No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCATCCTCTCTTGCAGGGCCAGTCAGACAATTAGCAGCAACTTAGCCTGGTA23140.15666457464620143No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22760.15409186339444877No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22730.15388875461141566No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGTCTGCAGTCTGAAGATT22610.15307631947928324No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22470.15212847849179542No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22380.1515191521426961No Hit
GTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGGGGAGGCCAGTTATTATACTG22360.1513837462873407No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22120.14975887602307586No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21840.14786319404810022No Hit
AGCAAATACAGTGTGTTCTTGGAATTGTCTCTGGAGATGGTGAACCGGGC21710.1469830559882901No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA21310.14427493888118204No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21310.14427493888118204No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC21030.1423792569062064No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20990.1421084451954956No Hit
GACTTCACTCTCACCATCAACAGTCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG20680.14000965443748684No Hit
GAGTGAAGTCTGTCCCAGACCCACTCCCACTGAACCTGACTGGGATACCA20660.13987424858213143No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCCCCTCACTTTCGGCGG20570.1392649222330321No Hit
CCACTATCCCCGACCGTTTTCGCACAGAAATATTGGGCCGTGTCCTCGGC20140.13635369634289096No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT20100.13608288463218013No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCC19870.134525717295593No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19780.13391639094649369No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19550.13235922360990657No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGACTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTCCCA19400.13134367969474106No Hit
GGTCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC19320.13080205627331942No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19260.13039583870725321No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC19250.13032813577957553No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT18360.12430257521626006No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18220.12335473422877224No Hit
CTCCTGTGAAGCCTCTGGATTCACCTTTACTTCCTATGCCATGACTTGGGTCCGCCAGGCTCCAGGGAAGGGG18090.12247459616896214No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAAGTCATGGCATAGGAAG17980.12172986396450743No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17820.1206466171216642No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17770.12030810248327568No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17710.11990188491720946No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17610.11922485564043246No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT17610.11922485564043246No Hit
CTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTCCCACTGAACCT17520.11861552929133315No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT17500.11848012343597773No Hit
CCATCAACAGTCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAG17380.11766768830384533No Hit
CTCTTGCAGGGCCAGTCAGACAATTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGTCTCCCAGGCT17180.11631362975029129No Hit
CTACGCAGACTCCGTGAGGGCCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACACTGTATTTGCTAATGAA16820.11387632435389404No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT16460.11143901895749678No Hit
GGATACCAGAGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGACTGGCCAGGTTTCTGCTGGTACCAGG16260.11008496040394275No Hit
GGGGAAAGAGTCATCCTCTCTTGCAGGGCCAGTCAGACAATTAGCAGCAACTTAGCCTGGTACCAGCAGAAAC16160.10940793112716574No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAAGTGCCCAGTGTGAGGTGCAGTTGTTGGAGT16080.10886630770574413No Hit
GTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTCCCACTGAACCTGA16060.10873090185038871No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGAAGCCTCTGGATTCACCTTTACTTCCTATGCCATGACTTGGGTCCG16040.1085954959950333No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15990.1082569813566448No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15870.1074445462245124No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15800.10697062573076849No Hit
CCTTTACTTCCTATGCCATGACTTGGGTCCGCCAGGCTCCAGGGAAGGGG15780.10683521987541306No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT15680.10615819059863606No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCTCTGGTATCCCA15600.10561656717721443No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15490.10487183497275973No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGGTGAGGAGACGGTGACCGTGGTCCCCTTG15420.10439791447901582No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15410.1043302115513381No Hit
CCTTGGGGCTGGTCGGGGATGGTGAGGAGACGGTGACCGTGGTCCCCTTG15390.1041948056959827No Hit
CCCTGAGACTCTCCTGTGAAGCCTCTGGATTCACCTTTACTTCCTATGCC15120.10236682664868477No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15100.10223142079332936No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15070.10202831201029626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)