FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477041
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCAT52400.3547633410311562No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48680.32957785193505124No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGTTGATGGTGAGAGTGAAGT44570.3017519486595159No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA41330.2798162000919406No Hit
ATTACATACTACGCAGACTCCGTGAGGGCCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACACTGTATTTG39820.26959305801260763No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT37890.25652639297081126No Hit
GTATGTAATAGCACCACTTCCACTAATAGCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAAGT34240.23181482436845016No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33780.2287004896952759No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32840.22233641449357194No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28480.19281793802609407No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28420.19241172046002786No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28290.19153158240021773No Hit
GGACAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG28250.19126077068950695No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28040.1898390092082752No Hit
GTGTGAGGTGCAGTTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGAAGC27930.18909427700382048No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT27370.18530291305386917No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27220.18428736913870367No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25900.17535058268524706No Hit
CATCTATGGTGCATCCACCAGGGCCTCTGGTATCCCAGTCAGGTTCAGTGGGAGTGGGTCTGGGACAGACTTCA24830.168106369423733No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCATCCTCTCTTGCAGGGCCAGTCAGACAATTAGCAGCAACTTAGCCTGGTA24000.16248702642648377No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCATCCTCTCTTGCAGGGCCAGTCAGACAATTAGCAG23800.16113296787292972No Hit
GTATTTGCTAATGAACAGCCTGAGAGCCGAGGACACGGCCCAATATTTCT23760.16086215616221894No Hit
CCATAGATGAGGAGCCTGGGAGACTGGCCAGGTTTCTGCTGGTACCAGGC23640.1600497210300865No Hit
GTCCCAGACCCACTCCCACTGAACCTGACTGGGATACCAGAGGCCCTGGT23330.15795093027207774No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22340.1512483404319853No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC22200.15030049944449747No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22050.14928495552933194No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGTCTGCAGTCTGAAGATT22020.14908184674629885No Hit
CTGGTATCCCAGTCAGGTTCAGTGGGAGTGGGTCTGGGACAGACTTCACT22010.14901414381862113No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21320.14434264180885975No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21030.1423792569062064No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20910.14156682177407398No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20660.13987424858213143No Hit
GTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGGGGAGGCCAGTTATTATACTG20480.1386555958839328No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20200.13675991390895717No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20140.13635369634289096No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19960.1351350436446923No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19950.13506734071701462No Hit
GACTTCACTCTCACCATCAACAGTCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG19830.1342549055848822No Hit
GAGTGAAGTCTGTCCCAGACCCACTCCCACTGAACCTGACTGGGATACCA19770.133848688018816No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19600.13269773824829506No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19520.13215611482687345No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19410.13141138262241875No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18980.12850015673227758No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18740.12687528646801274No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGACTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTCCCA18690.12653677182962422No Hit
GGTCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC18620.1260628513358803No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCCCCTCACTTTCGGCGG18270.12369324886716075No Hit
CCACTATCCCCGACCGTTTTCGCACAGAAATATTGGGCCGTGTCCTCGGC18230.12342243715644996No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17790.12044350833863109No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCC17670.11963107320649867No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17610.11922485564043246No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17330.11732917366545682No Hit
AGCAAATACAGTGTGTTCTTGGAATTGTCTCTGGAGATGGTGAACCGGGC17250.1167875502440352No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT17250.1167875502440352No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC17140.11604281803958048No Hit
CTCCTGTGAAGCCTCTGGATTCACCTTTACTTCCTATGCCATGACTTGGGTCCGCCAGGCTCCAGGGAAGGGG16850.11407943313692713No Hit
GGGGAAAGAGTCATCCTCTCTTGCAGGGCCAGTCAGACAATTAGCAGCAACTTAGCCTGGTACCAGCAGAAAC16790.11367321557086094No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16710.11313159214943931No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAAGTCATGGCATAGGAAG16640.1126576716556954No Hit
CCATCAACAGTCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAG16390.11096509846375287No Hit
CTCTTGCAGGGCCAGTCAGACAATTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGTCTCCCAGGCT16350.11069428675304206No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT15970.1081215755012894No Hit
CTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTCCCACTGAACCT15860.10737684329683468No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGAAGCCTCTGGATTCACCTTTACTTCCTATGCCATGACTTGGGTCCG15750.10663211109237997No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15570.10541345839418136No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAAGTGCCCAGTGTGAGGTGCAGTTGTTGGAGT15540.10521034961114824No Hit
GTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTCCCACTGAACCTGA15460.10466872618972661No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT15460.10466872618972661No Hit
CTACGCAGACTCCGTGAGGGCCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACACTGTATTTGCTAATGAA15240.1031792617808172No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15110.10229912372100707No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGACAAATAGTGATGACGCAGTC15020.10168979737190775No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15020.10168979737190775No Hit
GGATACCAGAGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGACTGGCCAGGTTTCTGCTGGTACCAGG14990.10148668858887466No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14790.10013263003532061No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT14790.10013263003532061No Hit
CCTTTACTTCCTATGCCATGACTTGGGTCCGCCAGGCTCCAGGGAAGGGG14780.10006492710764292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)