FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1717734
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC64820.3773576118304697No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62960.3665293927930634No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50740.295389158041932No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47290.27530455821448485No Hit
GACTTACAGTCTCCCCTACACGTTTGGCCAGGGGACCAAGGTGGAGATCAAAAGAACTGTGGCTGCACCAT47250.2750716932889493No Hit
GATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGTTTCAGTGGCAGTGGTTCTGGGACAGACTTCA42940.2499804975624864No Hit
GTTCTGGGACAGACTTCACTCTCACCATCAGTAGTCTGCAACCTGACGATTTTGCAACTTTTTACTGTCAACAGA42060.24485746920070278No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41670.2425870361767305No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG41510.24165557647458802No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT41390.2409569816979812No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA40690.23688184550110786No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG40070.23327243915530577No Hit
CTGTAAGTCTGTTGACAGTAAAAAGTTGCAAAATCGTCAGGTTGCAGACTACTGATGGTGAGAGTGAAGTCTGTC39020.22715973485999577No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37990.22116346302745363No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT35980.2094620005192888No Hit
CCCCTACACGTTTGGCCAGGGGACCAAGGTGGAGATCAAAAGAACTGTGG35470.20649297271870967No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34120.19863378148188252No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34010.19799340293665957No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAGAAGCAATTTAAATTGGTATCAGCAGAAA33990.19787697047389177No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33870.19717837569728489No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32650.19007599546844856No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG31970.18611729173434302No Hit
GTCCCAGAACCACTGCCACTGAAACTTGATGGGACCCCACTTTCCAAACT31860.1854769131891201No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31720.18466188594974542No Hit
GACTTCACTCTCACCATCAGTAGTCTGCAACCTGACGATTTTGCAACTTTTTACTGTCAACAGACTTACAGTCTC31510.1834393450906834No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAGAAG30940.18012101990180085No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30770.1791313439682745No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30560.17790880310921248No Hit
GTGTAGGGGAGACTGTAAGTCTGTTGACAGTAAAAAGTTGCAAAATCGTC30440.17721020833260565No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC29470.1715632338883669No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29180.16987496317823367No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT28700.16708058407180623No Hit
GAGTGAAGTCTGTCCCAGAACCACTGCCACTGAAACTTGATGGGACCCCA28680.1669641516090384No Hit
CTACTGATGGTGAGAGTGAAGTCTGTCCCAGAACCACTGCCACTGAAACT27130.1579406357445332No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT26810.15607771634024825No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCTTTTGATCTCCACCTTGGTCCCCTGGCCAAACGTGTAGGGGAGA26490.15421479693596332No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26400.15369085085350817No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26060.15171149898645542No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT25790.15013966073909No Hit
CAGTAATACAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATA25260.14705420047574302No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24600.14321192920440534No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24500.14262976689056628No Hit
GTGCAGCCACAGTTCTTTTGATCTCCACCTTGGTCCCCTGGCCAAACGTG24090.1402429014038262No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24000.13971895532137105No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT23210.1351198730420426No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23200.1350616568106587No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22200.12924003367226822No Hit
CTCCTGATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGTTTCAGTGGCAGTGGTTCTGGGACAGA22140.1288907362839648No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22110.12871608758981307No Hit
GTATTACTGTGCGAGACATAGGGATGTCAGTGGCTGGTACGGCCCCTTTGACCTCTGGGGCCAGGGAACCCTGGT21980.12795927658182232No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21610.12580527602061786No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20830.1212644099726733No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC20820.1212061937412894No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20530.11951792303115617No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20480.11922684187423663No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20310.11823716594071026No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA20310.11823716594071026No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT19910.11590851668535408No Hit
ATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGTTTCAGTGGCAGTGGTTCTGGGACAGACTTCACTCTCA19880.11573386799120236No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19800.11526813814013112No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19710.114744192057676No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19700.11468597582629209No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19650.11439489466937254No Hit
AGTCAGAACATTAGAAGCAATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATG19630.11427846220660476No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19440.11317235381031056No Hit
CGTTTGGCCAGGGGACCAAGGTGGAGATCAAAAGAACTGTGGCTGCACCA19020.11072727209218657No Hit
CTCCTGTGCAGCCTCGGGATTCACCTTTGATGCATATGGCATGAGCTGGGTCCGCCAACTTCCAGGGAGGGGC18950.11031975847249924No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18910.1100868935469636No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18750.10915543384482115No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18690.10880613645651771No Hit
CACCATCAGTAGTCTGCAACCTGACGATTTTGCAACTTTTTACTGTCAAC18540.10793289298575914No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18370.10694321705223278No Hit
GGCCAAACGTGTAGGGGAGACTGTAAGTCTGTTGACAGTAAAAAGTTGCA18340.10676856835808105No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18330.10671035212669715No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18240.106186406044242No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18180.10583710865593858No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18030.10496386518518No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17890.10414883794580534No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17810.1036831080947341No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17780.1035084594005824No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17740.10327559447504678No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17520.10199483738460087No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT17410.10135445883937794No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATTGCTTCTA17320.10083051275692279No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17290.10065586406277106No Hit
GGTTATGTGGACTCTGTGAAGGGACGATGCACCATCTCCAGAGACAACGC17210.10019013421169982No Hit
CCTTGGTCCCCTGGCCAAACGTGTAGGGGAGACTGTAAGTCTGTTGACAG17200.10013191798031593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)