FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1717734
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64570.3759022060458721No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54800.31902494798379727No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50820.29585488789300324No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50030.29125580561367476No Hit
GACTTACAGTCTCCCCTACACGTTTGGCCAGGGGACCAAGGTGGAGATCAAAAGAACTGTGGCTGCACCAT46320.2696575837702461No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43740.25463779607319875No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG43390.2526002279747621No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42830.24934011901726344No Hit
GTTCTGGGACAGACTTCACTCTCACCATCAGTAGTCTGCAACCTGACGATTTTGCAACTTTTTACTGTCAACAGA41940.24415887442409595No Hit
GATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGTTTCAGTGGCAGTGGTTCTGGGACAGACTTCA41800.24334384718472127No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39540.23018697889195883No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA38960.22681043747169236No Hit
CTGTAAGTCTGTTGACAGTAAAAAGTTGCAAAATCGTCAGGTTGCAGACTACTGATGGTGAGAGTGAAGTCTGTC37600.21889303000348134No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT36390.21184886600602884No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAGAAGCAATTTAAATTGGTATCAGCAGAAA34850.20288356637290758No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34770.20241783652183631No Hit
CCCCTACACGTTTGGCCAGGGGACCAAGGTGGAGATCAAAAGAACTGTGG33460.1947915102105448No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT33060.19246286095518864No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32750.1906581577822876No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32430.18879523837800263No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAGAAG32070.18669945404818208No Hit
GACTTCACTCTCACCATCAGTAGTCTGCAACCTGACGATTTTGCAACTTTTTACTGTCAACAGACTTACAGTCTC31970.18611729173434302No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31740.18477831841251321No Hit
GTCCCAGAACCACTGCCACTGAAACTTGATGGGACCCCACTTTCCAAACT31620.18407972363590638No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG31260.1819839393060858No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31000.1804703172901043No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT30170.1756383700852402No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29830.17365901821818744No Hit
GTGTAGGGGAGACTGTAAGTCTGTTGACAGTAAAAAGTTGCAAAATCGTC29690.17284399097881278No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29270.1703989092606888No Hit
GAGTGAAGTCTGTCCCAGAACCACTGCCACTGAAACTTGATGGGACCCCA28650.1667895029148867No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27750.1615500420903353No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC27250.15863923052114007No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26750.15572841895194484No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26410.15374906708489208No Hit
CTACTGATGGTGAGAGTGAAGTCTGTCCCAGAACCACTGCCACTGAAACT26020.1514786340609198No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT25830.1503725256646256No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25800.1501978769704739No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25610.14909176857417972No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCTTTTGATCTCCACCTTGGTCCCCTGGCCAAACGTGTAGGGGAGA25180.14658847062467179No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25170.1465302543932879No Hit
GTGCAGCCACAGTTCTTTTGATCTCCACCTTGGTCCCCTGGCCAAACGTG24550.1429208480474858No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT24350.1417565234198077No Hit
CTCCTGATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGTTTCAGTGGCAGTGGTTCTGGGACAGA23360.13599311651280116No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23310.13570203535588163No Hit
CAGTAATACAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATA22870.13314052117498984No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22640.13180154785316003No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22030.12825035773874185No Hit
GTATTACTGTGCGAGACATAGGGATGTCAGTGGCTGGTACGGCCCCTTTGACCTCTGGGGCCAGGGAACCCTGGT21780.12679495195414425No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21710.1263874383344569No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21370.12440808646740414No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC20950.12196300474928015No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA20800.12108976127852158No Hit
ATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGTTTCAGTGGCAGTGGTTCTGGGACAGACTTCACTCTCA20340.11841181463486197No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20250.11788786855240682No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19900.11585030045397017No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19820.11538457060289893No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19680.11456954336352426No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT19680.11456954336352426No Hit
CTCCTGTGCAGCCTCGGGATTCACCTTTGATGCATATGGCATGAGCTGGGTCCGCCAACTTCCAGGGAGGGGC19550.11381273235553352No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19500.11352165119861397No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19450.11323057004169446No Hit
GGCCAAACGTGTAGGGGAGACTGTAAGTCTGTTGACAGTAAAAAGTTGCA19360.11270662395923932No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19290.11229911033955198No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19260.11212446164540028No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19250.11206624541401637No Hit
AGTCAGAACATTAGAAGCAATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATG18410.10717608197776839No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18340.10676856835808105No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18330.10671035212669715No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18310.10659391966392932No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18140.10560424373040296No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18080.10525494634209955No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17800.1036248918633502No Hit
CACCATCAGTAGTCTGCAACCTGACGATTTTGCAACTTTTTACTGTCAAC17750.10333381070643068No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17660.10280986462397554No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17530.10205305361598478No Hit
GGTTATGTGGACTCTGTGAAGGGACGATGCACCATCTCCAGAGACAACGC17490.10182018869044915No Hit
CGTTTGGCCAGGGGACCAAGGTGGAGATCAAAAGAACTGTGGCTGCACCA17280.10059764783138717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)