FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200355
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30120.25092576779369435No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24160.20127378983717317No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23760.1979414423233127No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21970.18302918719878702No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20460.1704495753339637No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT18530.15437099857958686No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC18520.15428768989174035No Hit
GTGTTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC18270.15220497269557756No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17540.14612343848278217No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17130.14270778228107517No Hit
GTCCCAGACCCACTGGCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT17130.14270778228107517No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16520.13762595232243796No Hit
GCCTAGAGCCTGAAGATTATGCCGTTTATTACTGTCAGCAGCGTTTAGGC16100.13412698743288443No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15730.13104456598256348No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT15680.13062802254333095No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCTACTTAGCCTGGTA15650.1303780964797914No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14760.12296362326145183No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14520.12096421475313553No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14490.120714288689596No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14460.12046436262605645No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13750.11454944578895411No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13610.11338312415910294No Hit
GTCAGAGTGTTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATCATG12940.10780144207338663No Hit
GCCTAAACGCTGCTGACAGTAATAAACGGCATAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT12880.10730158994630755No Hit
GTTTATTACTGTCAGCAGCGTTTAGGCTGGCCTCCGATCACCTTCGGCCA12770.10638519437999591No Hit
GTAATAAACGGCATAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGGCA12770.10638519437999591No Hit
GTATCAACGCAGAGTACGGGAATATCAACCCGTTGTCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGA12540.10446909455952615No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12490.10405255112029357No Hit
GCGTTTAGGCTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT12480.10396924243244705No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTATGCCGTTTATTACTGTCAGCAGCGTTTAGGCTGG12460.10380262505675406No Hit
GAGTGAAGTCTGTCCCAGACCCACTGGCACTGAACCTGGCTGGGATGCCA12040.10030366016720053No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)