FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304005
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATCCAAGTTACAGAGTGGGGTCCCATTAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA60270.4621914793271498No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57840.4435565814548257No Hit
ACAGTACACCACTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGA54170.4154125175900399No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51310.39348008634936216No Hit
GTATAGATCAGGAGTTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAGTT41550.3186337475699863No Hit
GTGTACTGTAACTCTGTTGACAGTAGAAAGTCGCAAAATCTTCAGGTTGCAAACTGTTAATGGTGAGAGTGAATT41070.31495278008903344No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38630.29624119539418947No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37580.28818907902960494No Hit
GAGTTACAGTACACCACTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT36880.28282100145321526No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34270.2628057407755338No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33680.25828121824686257No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33630.2578977841342633No Hit
CCATTAACAGTTTGCAACCTGAAGATTTTGCGACTTTCTACTGTCAACAG33570.2574376631991442No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33010.25314320113803246No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29630.22722305512632235No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28770.22062798838961506No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27960.21441635576550702No Hit
GAATTCACTCTCACCATTAACAGTTTGCAACCTGAAGATTTTGCGACTTTCTACTGTCAACAGAGTTACAGTACA27510.21096544875211365No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27030.20728448127116075No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26630.20421700837036666No Hit
CTGTAACTCTGTTGACAGTAGAAAGTCGCAAAATCTTCAGGTTGCAAACTGTTAATGGTGAGAGTGAATTCTGTC26350.20206977733981082No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAAAGTGACCATCACTT25990.19930905172909613No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25870.19838880985885793No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24670.18918639115647562No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC24500.18788271517363814No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24230.18581217096560212No Hit
CTGTTAATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCT23610.1810575879693713No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTCCGCCGAAAGTG23540.18052078021173232No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22930.17584288403802134No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22430.17200854291202872No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22400.17177848244446917No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21450.1644932343050832No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGTGGTGTACTGTAACTCTGTTGACA21160.16226931645200748No Hit
GACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT21140.16211594280696776No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTCCGCCGAAAGTGAGTGGTGTA19840.15214665587938697No Hit
GTACACCACTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACT19820.15199328223434727No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19790.15176322176678772No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19660.1507662930740296No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19630.15053623260647006No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19270.1477755069957554No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19090.14639514419039804No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19020.14585833643275908No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT19000.14570496278771938No Hit
CCCCTAAACTCCTGATCTATACTGCATCCAAGTTACAGAGTGGGGTCCCA18840.14447797362740175No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18740.14371110540220322No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18680.14325098446708412No Hit
ATACTGCATCCAAGTTACAGAGTGGGGTCCCATTAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCA17180.13174796108910625No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17050.13075103239634817No Hit
AAGTTACAGAGTGGGGTCCCATTAAGGTTCAGTGGCAGTGGATCTGGGAC16740.12837374089823275No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16660.12776024631807392No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTTAATGGGACCCCA16330.1252295811749188No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16190.12415596565964086No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16160.12392590519208133No Hit
CAATAATATATGGCCGTGTCGTCAGATCTCAGATTGGAAAGTTGCAGGTA16090.12338909743444236No Hit
GTCTGCATCTGTAGGAGACAAAGTGACCATCACTTGCCGGGCGAGTCAAT16040.12300566332184308No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15750.12078174546876737No Hit
ATCTATTAGGGATTATTTAAACTGGTATCAGCAGAAACCAGGGGAAGCCCCTAAACTCCTGATCTATACTGCATC15690.12032162453364827No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15450.11848114079317182No Hit
GTTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAGTTTAAATAATCCCTA15370.11786764621301297No Hit
GTAGGAGACAAAGTGACCATCACTTGCCGGGCGAGTCAATCTATTAGGGATTATTTAAACTGGTATCAGCAGAAA15310.11740752527789387No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15250.11694740434277474No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15190.11648728340765564No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15190.11648728340765564No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15170.11633390976261593No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15010.1151069206022983No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC15010.1151069206022983No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14750.11311306321678215No Hit
GTGGTGTACTGTAACTCTGTTGACAGTAGAAAGTCGCAAAATCTTCAGGT14710.11280631592670275No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14600.11196276087898437No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14380.1102756507835476No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14250.10927872209078952No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14210.10897197480071012No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13920.10674805694763441No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13880.10644130965755501No Hit
CATTAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATTAACAGTTTGCAACCTGAAGATTTTG13670.10483088638463808No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13660.10475419956211825No Hit
ACCTTAATGGGACCCCACTCTGTAACTTGGATGCAGTATAGATCAGGAGTTTAGGGGCTTCCCCTGGTTTCTG13520.1036805840468403No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13440.10306708946668149No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13430.10299040264416164No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13390.10268365535408222No Hit
CTGTAACTTGGATGCAGTATAGATCAGGAGTTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAGTTTAAATAATC13270.101763413483844No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13270.101763413483844No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13250.1016100398388043No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13190.10114991890368519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)