FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304005
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58160.44601055977546095No Hit
GATCTATACTGCATCCAAGTTACAGAGTGGGGTCCCATTAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA57630.4419461581819088No Hit
ACAGTACACCACTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGA48620.3728513310915219No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42810.3282962872074877No Hit
GTGTACTGTAACTCTGTTGACAGTAGAAAGTCGCAAAATCTTCAGGTTGCAAACTGTTAATGGTGAGAGTGAATT41200.31594970878179146No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40990.3143392855088746No Hit
GTATAGATCAGGAGTTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAGTT38390.294400711653713No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37500.2875755844494461No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37060.2842013642585726No Hit
GAGTTACAGTACACCACTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT35860.27499894555619037No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33290.2552904321685883No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31030.2379592102791017No Hit
CCATTAACAGTTTGCAACCTGAAGATTTTGCGACTTTCTACTGTCAACAG29340.22499913727324666No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28770.22062798838961506No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28530.2187875046491386No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28330.21725376819874156No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26990.20697773398108138No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26510.20329676650012846No Hit
GAATTCACTCTCACCATTAACAGTTTGCAACCTGAAGATTTTGCGACTTTCTACTGTCAACAGAGTTACAGTACA26060.19984585948673508No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26040.1996924858416954No Hit
CTGTAACTCTGTTGACAGTAGAAAGTCGCAAAATCTTCAGGTTGCAAACTGTTAATGGTGAGAGTGAATTCTGTC25460.19524465013554396No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAAAGTGACCATCACTT24920.19110356171947193No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24350.18673241283584036No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23910.18335819264496683No Hit
CTGTTAATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCT23910.18335819264496683No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTCCGCCGAAAGTG23750.1821312034846492No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23490.18013734609913307No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23470.17998397245409334No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC22550.17292878478226695No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21960.16840426225359564No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20900.16027545906649132No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20490.15713129934317738No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20330.15590431018285972No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20310.15575093653782002No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19950.15299021092710535No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19690.1509963535415892No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGTGGTGTACTGTAACTCTGTTGACA19630.15053623260647006No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19590.15022948531639066No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTCCGCCGAAAGTGAGTGGTGTA18960.14539821549763998No Hit
GACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT18900.14493809456252085No Hit
GTACACCACTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACT18790.14409453951480247No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18210.13964670380865105No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18210.13964670380865105No Hit
CCCCTAAACTCCTGATCTATACTGCATCCAAGTTACAGAGTGGGGTCCCA18080.13864977511589296No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17190.13182464791162612No Hit
GTCTGCATCTGTAGGAGACAAAGTGACCATCACTTGCCGGGCGAGTCAAT17170.1316712742665864No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17000.13036759828374891No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16740.12837374089823275No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16430.1259964494001173No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTTAATGGGACCCCA16350.1253829548199585No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16340.12530626799743866No Hit
ATACTGCATCCAAGTTACAGAGTGGGGTCCCATTAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCA16140.12377253154704161No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15990.12262222920924383No Hit
GTGGTGTACTGTAACTCTGTTGACAGTAGAAAGTCGCAAAATCTTCAGGT15850.1215486136939659No Hit
GTTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAGTTTAAATAATCCCTA15190.11648728340765564No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15140.11610384929505639No Hit
AAGTTACAGAGTGGGGTCCCATTAAGGTTCAGTGGCAGTGGATCTGGGAC15080.11564372835993726No Hit
ATCTATTAGGGATTATTTAAACTGGTATCAGCAGAAACCAGGGGAAGCCCCTAAACTCCTGATCTATACTGCATC14830.11372655779694096No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14760.113189750039302No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14760.113189750039302No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14550.1115793267663851No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14360.1101222771385079No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14290.10958546938086894No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14270.10943209573582924No Hit
GTAGGAGACAAAGTGACCATCACTTGCCGGGCGAGTCAATCTATTAGGGATTATTTAAACTGGTATCAGCAGAAA14240.10920203526826969No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14220.10904866162322997No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14210.10897197480071012No Hit
CAATAATATATGGCCGTGTCGTCAGATCTCAGATTGGAAAGTTGCAGGTA14120.10828179339803146No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13800.10582781507739618No Hit
CTCCTGATCTATACTGCATCCAAGTTACAGAGTGGGGTCCCATTAAGGTT13750.10544438096479691No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13600.10429407862699915No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13450.10314377628920135No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13440.10306708946668149No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13430.10299040264416164No Hit
GTCCTGTCCCTGTCGTCACCGTAGTCCCCTGTCCTTGCGCACAATAATAT13380.10260696853156238No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13350.10237690806400282No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13290.10191678712888372No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13240.10153335301628444No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13210.1013032925487249No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13050.10007630338840726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)