FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897592
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28780.3206356562892717No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25040.2789686182586298No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20710.23072843786486513No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19580.2181391991015963No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19430.2164680612126668No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGGCAGGCTATTAGCAA17800.19830836281963296No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16520.18404798616743465No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16290.18148557473774274No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15420.1717929749819517No Hit
GATCTATGCAGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAACGGCAGTGGATCTGGGACAGATTTCA15320.1706788830559987No Hit
GGCTAACAGTTTTCCGTACACTTTCGGCGGAGGGACCACGGTGGAGCTCAAACGAACTGTGGCTGCACCAT15040.16755942566333032No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14880.16577687858180554No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCACCGTGGTCCCTCCGCCGAAAGTG14800.16488560504104313No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14730.16410574069287603No Hit
GGCTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAAGCAGGGAAAGCCCCTAAGCTCCTGATCTATGCAGCATC14310.15942655460387348No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14250.1587580994483017No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC14160.15775541671494397No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14080.1568641431741816No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTT13930.1551930052852521No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13920.15508159609265681No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13850.1543017317444897No Hit
GTAATAAACAGCCATGTCCTCAGCGCTCAGGCTGCCCATTTGAAGATACA13770.1534104582037273No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCACCGTGGTCCCTCCGCCGAAAGTGTACGGAAAA13660.152184957085179No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13340.14861986292212945No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13130.14628026987762816No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12880.14349504006274566No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12500.13926149074412428No Hit
CTCCTGATCTATGCAGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT12450.1387044447811478No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12320.1372561252774089No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT11080.12344138539559177No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGTTGAACCTTGATGGGACCCCA11070.12332997620299647No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10790.12021051881032807No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10520.11720247061025499No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGCTTTCTGCTGATACCAGGC10480.1167568338398738No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGCTTTCTGCTGATACCAGGCTAACC10330.11508569595094431No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9880.11007228228415583No Hit
CACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTTCCGTACACTTT9660.10762128004705923No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9600.10695282489148746No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9530.10617296054332036No Hit
ACCCTATCTCGCGCACAGTAATAAACAGCCATGTCCTCAGCGCTCAGGCTGCCCATTTGAAGATACAGTGTGTT9390.10461323184698615No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9340.10405618588400967No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9120.10160518364691308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25800.039.562391
GTGGTAT3250.032.453331
TATCAAC32150.031.53672
TCAACGC32600.031.310114
ATCAACG32550.031.253683
CAACGCA32950.030.9775315
ACGAACC1251.0004442E-1030.92115652
AACGCAG33600.030.4812226
TGTTAGA9500.029.51989470
CCGAAAT952.9293915E-729.34979447
ACGCAGA35700.028.6818147
CGCAGAG35500.028.5559258
GTAGTTT1904.2604734E-728.11418370
AGAGTAC35850.027.99402811
CAGAGTA36850.027.32667210
CTAGCAC500.0082768527.2189223
GCAGAGT37700.026.7993019
GAGTTTA3750.026.3116231
AACGCCA659.387469E-426.172045
ATAGCGG1559.404175E-1025.52541763
Produced by FastQC (version 0.11.5)