FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066436
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58350.5471495710947493No Hit
GTCTTCAGGCTGGAGGTTGCTGATGGTGAGAGTGAAGTCAGTCCCGAATCCACTGCCACTGAAGCGTGGCGGGAC48560.4553484691064443No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48460.4544107663282185No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38580.36176573183951033No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35280.3308215401580592No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33940.3182563229298336No Hit
GCCTGAAGACTGTGCAACTTACTATTGTCAACAGGCTAATGTCTTTCCTC33070.3100983087592692No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31070.2913442531947534No Hit
AGTCAGGACACAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTC31060.2912504829169308No Hit
GTTTATGAATAGGTGGCTAGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAACTCCTCATCTATGCTGGAGC30730.28815606374878566No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30270.2838426309689471No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29850.2799042793003987No Hit
GTGCAGCCACAGTTCGATTGATGTCCACCTTGGTCCCTCCGCCGAAAGTG29170.27352790040846336No Hit
CCTGAAGACTGTGCAACTTACTATTGTCAACAGGCTAATGTCTTTCCTCG28960.27155872457418917No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27980.2623692373475764No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27360.2565554801225765No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27100.2541174528991894No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25960.24342764122741545No Hit
GCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25150.2358322487237865No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25010.23451946483427044No Hit
GGCTAATGTCTTTCCTCGCACTTTCGGCGGAGGGACCAAGGTGGACATCAATCGAACTGTGGCTGCACCAT24920.2336755323338672No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23110.2167031120479804No Hit
CTATAGGGGACAGAGTCACCATGACTTGTCGGGCGAGTCAGTTTATGAAT22690.21276476037943204No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22460.21060804398951274No Hit
GACTTCACTCTCACCATCAGCAACCTCCAGCCTGAAGACTGTGCAACTTACTATTGTCAACAGGCTAATGTCTTT22310.20920148982217407No Hit
ATAGGGGACAGAGTCACCATGACTTGTCGGGCGAGTCAGTTTATGAATAGGTGGCTAGCCTGGTATCAGCAGAAA22240.20854509787741599No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22140.20760739509919018No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21760.20404412454193221No Hit
GAGTCACCATGACTTGTCGGGCGAGTCAGTTTATGAATAGGTGGCTAGCCTGGTATCAGCAGAAACCAGGAAAAG20620.19335431287015817No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20580.19297923175886786No Hit
GTAATAAACGGCCGTGTCTTCGGCGGTCACAGACGTCAGATTCAGGAAGAACCGATTCTTGGACGTGTCCACGGA19970.18725924481169054No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATTGATGTCCACCTTGGTCC19620.18397728508790026No Hit
CATCTATGCTGGAGCCACTTTGCAAAGTGGGGTCCCGCCACGCTTCAGTGGCAGTGGATTCGGGACTGACTTCA19590.18369597425443251No Hit
GTTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGCTAGCCACCTATTC19500.1828520417540293No Hit
GTCCGTGGACACGTCCAAGAATCGGTTCTTCCTGAATCTGACGTCTGTGACCGCCGAAGACACGGCCGTTTATTA18890.17713205480685199No Hit
ACTTACTATTGTCAACAGGCTAATGTCTTTCCTCGCACTTTCGGCGGAGGGACCAAGGTGGACATCAATCGAA18860.17685074397338427No Hit
GAGTGAAGTCAGTCCCGAATCCACTGCCACTGAAGCGTGGCGGGACCCCA18510.173568784249594No Hit
ACATTAGCCTGTTGACAATAGTAAGTTGCACAGTCTTCAGGCTGGAGGTTGCTGATGGTGAGAGTGAAGTCAGTC18330.17188091924878754No Hit
CTTCAGTGGCAGTGGATTCGGGACTGACTTCACTCTCACCATCAGCAACCTCCAGCCTGAAGACTGTGCAACTTA18180.17047436508144886No Hit
CAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT17990.16869272980281985No Hit
GGACACAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17950.16831764869152954No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17910.16794256758023923No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17730.16625470257943278No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17460.16372290507822318No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17320.16241012118870707No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16920.1586593100758039No Hit
CCCCTAAACTCCTCATCTATGCTGGAGCCACTTTGCAAAGTGGGGTCCCG16840.15790914785322327No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16600.15565866118548136No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16560.15528358007419105No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16490.154627188129433No Hit
ATTCATAAACTGACTCGCCCGACAAGTCATGGTGACTCTGTCCCCTATAG16290.15275178257298141No Hit
ATAGTAAGTTGCACAGTCTTCAGGCTGGAGGTTGCTGATGGTGAGAGTGAAGTCAGTCCCGAATCCACTGCCA15700.14721933618144922No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC15560.14590655229193314No Hit
GACCCAGTCAGGACACAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGG15350.14393737645765897No Hit
CACCATGACTTGTCGGGCGAGTCAGTTTATGAATAGGTGGCTAGCCTGGTATCAGCAGAAACCAGGAAAAGCCC15310.14356229534636866No Hit
CCTTGGTCCCTCCGCCGAAAGTGCGAGGAAAGACATTAGCCTGTTGACAATAGTAAGTTGCACAGTCTTCAGG14730.13812361923265906No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14720.13802984895483647No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14490.13587313256491718No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14450.13549805145362684No Hit
GTCAGGACACAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14280.133903956730643No Hit
GACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCC14200.13315379450806236No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14080.13202855117419143No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13870.13005937533991727No Hit
CCTCCAGCCTGAAGACTGTGCAACTTACTATTGTCAACAGGCTAATGTCTTTCCTCGCACTTTCGGCGGAGGGAC13760.1290279022838689No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13730.12874659145040115No Hit
ATGCTGGAGCCACTTTGCAAAGTGGGGTCCCGCCACGCTTCAGTGGCAGTGGATTCGGGACTGACTTCACTCTCA13500.12658987506048183No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13490.12649610478265924No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13380.12546463172661088No Hit
GTCTCCATCTTCCGTGTCGGCTTCTATAGGGGACAGAGTCACCATGACTT13360.12527709117096572No Hit
CTCCTCATCTATGCTGGAGCCACTTTGCAAAGTGGGGTCCCGCCACGCTTCAGTGGCAGTGGATTCGGGACTGA13350.12518332089314313No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13330.12499578033749799No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13320.1249020100596754No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13090.12274529366975609No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12920.12115119894677223No Hit
GTTTATTACTGTGCGAGAGAAGGAGATTACGGTGACTACTACTTTCACCA12910.12105742866894967No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12900.12096365839112708No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12900.12096365839112708No Hit
GGACCAAGGTGGACATCAATCGAACTGTGGCTGCACCATCTGTCTTCATC12850.12049480700201418No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12680.11890071227903035No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12660.11871317172338519No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12630.11843186088991746No Hit
ATTGTCAACAGGCTAATGTCTTTCCTCGCACTTTCGGCGGAGGGACCAAGGTGGACATCAATCGAACTGTGGCTG12500.11721284727822391No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12340.11571252283306264No Hit
GTCCACGGACAGGGCGACTCGACTCTTGAGGGAGGGGTTGTAGATGGTACTTCCCCCGACATGGACACGCCCAAT12160.11402465783225622No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12020.11271187394274011No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11970.11224302255362721No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11950.11205548199798206No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC11740.1100863061637079No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11640.10914860338548212No Hit
GACCAAGGTGGACATCAATCGAACTGTGGCTGCACCATCTGTCTTCATCT11540.10821090060725631No Hit
GCATAGATGAGGAGTTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGC11480.10764827894032085No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11460.1074607383846757No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11450.1073669681068531No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11370.10661680588427247No Hit
GCCACAGTTCGATTGATGTCCACCTTGGTCCCTCCGCCGAAAGTGCGAGG11140.10446008949435315No Hit
TGGTGACTCTGTCCCCTATAGAAGCCGACACGGAAGATGGAGACTGGGTC11140.10446008949435315No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11120.10427254893870799No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11110.10417877866088542No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG11000.10314730560483704No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10990.10305353532701446No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10990.10305353532701446No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10910.10230337310443384No Hit
ATTCGGGACTGACTTCACTCTCACCATCAGCAACCTCCAGCCTGAAGACTGTGCAACTTACTATTGTCAACAGG10910.10230337310443384No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10890.10211583254878867No Hit
GTGCAACTTACTATTGTCAACAGGCTAATGTCTTTCCTCGCACTTTCGGC10810.10136567032620805No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10800.10127190004838545No Hit
CCACTGAAGCGTGGCGGGACCCCACTTTGCAAAGTGGCTCCAGCATAGAT10750.10080304865927257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)